173,176 results
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Plasmid#117231PurposeBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella PneumoniaeDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1179 U6-reci Gag-Cas9 v2
Plasmid#201914PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)DepositorInsertGag-Cas9 v2
ExpressionMammalianPromoterCAGAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB_tre_Cas9
Plasmid#126029PurposePiggyBac cargo vector expressing doxycycline inducible Cas9DepositorInsertshumanized S. pyrogenes Cas9
Hygromycin resistance
UseCRISPR; PiggybacExpressionMammalianPromoterEF1 alpha and TREAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiSSTe4_dTomato_nlsdTomato (AAV PHP.eB)
Viral Prep#213944-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiSSTe4_dTomato_nlsdTomato (#213944). In addition to the viral particles, you will also receive purified pAAV_BiSSTe4_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the SST interneuron-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorTagsdTomatoAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Hygro
Plasmid#139462PurposeLentiviral vector with gRNA scaffold and hygromycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: hygroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAS-mCherry-NLS
Plasmid#87695PurposeGal4VP16 driven expression of mCherry-NLS in mammalian cellsDepositorInsertmCherry-NLS
Tagsnuclear localization signalExpressionMammalianAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOpen-MMLV_RT (mut H)
Plasmid#165546PurposeSingle 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.DepositorInsertMoloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
UseSynthetic BiologyAvailable SinceAug. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTXB1-nbOcIgG-Tn5
Plasmid#184285PurposeAnti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbOcIgG-Tn5
Available SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPK1520
Plasmid#65777PurposeHuman expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNADepositorInsertSpCas9 gRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-tag (m) Vector
Plasmid#101135PurposeExpression of protein of interest with SNAP-Tag in Mammalian cellsDepositorTypeEmpty backboneTagsSNAP-tag (SNAP26m)ExpressionMammalianAvailable SinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-hSyncytin-1
Plasmid#182440PurposehSyncytin-1DepositorAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-ChR2-mCherry
Plasmid#135634PurposeAAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas ServiceAAV PHP.eB, AAV1, and AAV9InsertChR2-mCherry
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-CRBN-midi
Plasmid#215330PurposeCRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader designDepositorInsertCRBN (CRBN Human)
Tags6xHis and TEVExpressionBacterialMutationcontains amino acids 41-187 followed by a Gly-Ser…PromoterlacIAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307 P2A GFP YAP1
Plasmid#193675PurposeConstitutive lentiviral expression of YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Flp
Plasmid#122969PurposeExpresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.DepositorInsertFlp recombinase
ExpressionBacterialPromoterpBADAvailable SinceAug. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (AAV1)
Viral Prep#100848-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (#100848). In addition to the viral particles, you will also receive purified pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
CaM1234(D20A/D56AD93A/D129A)/pIRES2-eGFP
Plasmid#111518PurposeElectrophysiology in HEK cellDepositorAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-Bag6
Plasmid#61836Purposeexpress N-terminally FLAG tagged human Bag6DepositorAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP
Plasmid#71237PurposeExpress sgRNA and dCas9-KRAB from 3rd generation lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A GFP
sgRNA
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
HyPer7.2DAAO-NLS
Plasmid#168302PurposeChemogenetic fluorescent H2O2 reporter targeted to the nucleusDepositorInsertHyPer7.2DAAO-NLS
ExpressionMammalianPromoterCMVAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pACryGFP
Plasmid#64022PurposeGateway destination vector with zebrafish aA-crystallin promoter driving GFP in lensDepositorInsertGFP
UseGatewayPromoteralpha-Acrystallin promoterAvailable SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
DHB-mVenus
Plasmid#136461PurposeFluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2DepositorAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBH001 (TadDE-T7RNAP)
Plasmid#247050PurposeExpresses the fusion of TadDE (dual deaminase) and T7RNAP for gene-specific mutagenesisDepositorInsertTadDE-T7RNAP (T7p07 Synthetic)
ExpressionBacterialAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBID2_UAS>MCS
Plasmid#194958PurposeDrosophila transgene expression under UAS/Gal4 control, PhiC31 transgenesisDepositorTypeEmpty backboneExpressionInsectMutation20 x UAS sequencesAvailable SinceOct. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBID2_MCS>Gal4
Plasmid#194961PurposeDrosophila Gal4 expression under introduced sequence control, PhiC31 transgenesisDepositorTypeEmpty backboneExpressionInsectMutationGal4.2Available SinceOct. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
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Flag-Keap1
Plasmid#28023DepositorAvailable SinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-mCherry
Plasmid#50459PurposeDouble floxed mCherry under the control of human synapsin promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertmCherry
UseAAVTagsN/APromoterhuman Synapsin 1Available SinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
PGK-H2BeGFP
Plasmid#21210DepositorAvailable SinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s
Plasmid#40753PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6s
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable SinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-OKSM
Plasmid#204498PurposeExpresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cellsDepositorAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis).iGluSnFR
Plasmid#41732PurposeSingle-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTagsMyc, PDGFR transmembrane helix (aa 513-561), and …ExpressionMammalianMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterCMVAvailable SinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-iGluSnFR4s-NGR-WPRE
Plasmid#234437PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorHas ServiceAAV1InsertiGluSnFR4s-NGR
UseAAVTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianPromoterSynapsinAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-IDH1-R132H-FLAG
Plasmid#66803PurposeTet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vectorDepositorInsertIDH1 (IDH1 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationR132HPromoterCMVAvailable SinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET303_sumocodo_GFP_codoopti
Plasmid#210393PurposeExpress SUMO-GFP in E. coliDepositorInsertGFP
TagsSUMO-tagExpressionBacterialAvailable SinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
MORF library without GFP and mCherry controls
Pooled Library#192821PurposeBarcoded transcription factor ORF library for high throughput screeningDepositorExpressionMammalianSpeciesHomo sapiensUseLentiviralAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (AAV8)
Viral Prep#137141-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (#137141). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Cre) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsEYFP (Flp-dependent)Available SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iSeroSnFR-Nlgn (AAV9)
Viral Prep#128485-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-iSeroSnFR-Nlgn (#128485). In addition to the viral particles, you will also receive purified pAAV-CAG-iSeroSnFR-Nlgn plasmid DNA. CAG-driven expression of a fluorescent reporter for serotonin (localized to dendrites). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV044
Plasmid#191824PurposePlasmid for the transformation of the cyanobacterium Arthrospira platensis at the KmR locus. pCPC600 promoter. With terminator. Golden Gate compatible. Selection: in A. platensis: KmR; E. coli: AmpRDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only