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32,656 results

Showing: 4601 - 4650 of 32656 results

  6. 6xHis-MBP-huMb2Cas12a

    Plasmid
    #174676
    Purpose
    Expresses 6xHis-MBP tagged Mb2Cas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huMb2Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    174676-plasmid-map-sequence-id-346137

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  12. pRelA*

    Plasmid
    #175590
    Purpose
    Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Tags
    Fused with a flexible GS linker to mVenus
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175590-plasmid-map-sequence-id-348362

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  14. pGTVL2-CRBN-TBD

    Plasmid
    #196369
    Purpose
    protein expression of the GST-tagged Cereblon Thalidomide binding domain
    Depositor
    Stefan Knapp
    Insert
    CRBN (CRBN Human)
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    contains only T319-T425
    Promoter
    T7
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196369-plasmid-map-sequence-id-385991

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  18. NEK1A-c011

    Plasmid
    #53661
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    NEK1A
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. T-IL2-12

    Plasmid
    #166564
    Purpose
    scFv of a human scaffold targeting Interleukin-2. Antigen coverage aa 21-153 of 153, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    166564-plasmid-map-sequence-id-327819

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  22. UBI-D4

    Plasmid
    #25222
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UBI-D4_2 (DPF2 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. pET24a-VHH-mCherry

    Plasmid
    #109421
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109421-plasmid-map-sequence-id-211587

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  26. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128940-plasmid-map-sequence-id-254597

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  27. KG#121

    Plasmid
    #110879
    Purpose
    Expresses the C. elegans unc-31 cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1
    Inserts
    rab-3 promoter
    unc-31 cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Mutation
    Missing first 408bp
    Available Since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110879-plasmid-map-sequence-id-212481

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  37. pColdII-SIRPT1-Bubalus bubalis

    Plasmid
    #75350
    Purpose
    Nt His-tag Bubalus bubalis Sacsin 86-1364
    Depositor
    Kalle Gehring
    Insert
    Sacsin Bubalus bubalis 86-1364
    Tags
    His-Tag
    Expression
    Bacterial
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75350-plasmid-map-sequence-id-142906

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  45. pHCL151

    Plasmid
    #134455
    Purpose
    TetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.
    Depositor
    Thomas Bernhardt
    Article
    Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111
    Insert
    linker-mScarlet-I for C-terminal fusion
    Expression
    Bacterial
    Promoter
    plac
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    134455-plasmid-map-sequence-id-270547

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  49. p2B-58

    Plasmid
    #177928
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Expression
    Bacterial and Yeast
    Promoter
    TET4 (contains four rtTA binding sites)
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177928-plasmid-map-sequence-id-349996

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  50. pSW002-Pc-mNeonGreen

    Plasmid
    #205016
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    mNeonGreen
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available Since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    205016-plasmid-map-sequence-id-424184

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Showing: 4601 - 4650 of 32656 results