76,230 results
-
-
pcDNA4-AMBRA1-3xFLAG
Plasmid#174157PurposeVector for expression of 3xFLAG-tagged wild-type AMBRA1DepositorInsertAMBRA1 (AMBRA1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX401-INK4A
Plasmid#121919PurposeDoxycycline-inducible expression of p16-INK4A product of CDKN2ADepositorInsertp16-INK4A (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE Tet ONAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Flag-c-Myc
Plasmid#102626PurposeExpresses c-Myc in mammalian cellsDepositorInsertMYC proto-oncogene, bHLH transcription factor (MYC Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
PDG459 V3
Plasmid#226958PurposeCBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307-CRBN-3F-BirA
Plasmid#220146PurposeExpresses CRBN-BirA for CRBN substrate identification by E-STUBDepositorInsertCereblon (CRBN Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationPromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-Tet1-CD
Plasmid#83341PurposeTo introduce MS2 coat protein fused Tet1-CDDepositorInsertMS2-Tet1-CD
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3
Plasmid#21073PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsEGFPExpressionMammalianMutationFirst ATG is not included.PromoterCMVAvailable sinceMay 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
ppyCAG_RNaseH1_WKKD
Plasmid#111905PurposeExpress WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybridsDepositorInsertRNASEH1 (RNASEH1 Human)
UseTagsV5ExpressionMammalianMutationChanged D 210 to N, W 43 to A, K 59 to A, K 60 to…PromoterAvailable sinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Integrin β5-GFP
Plasmid#205090PurposeExpress GFP-tagged human integrin β5 in mammalian cellsDepositorInsertIntegrin beta 5 (ITGB5 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMEH343 CD63 MS2 HA
Plasmid#168217PurposeMammalian expression of tetraspanin CD63 fused to the MS2 dimer and C-terminal HA tagDepositorInsertCD63 (CD63 Human)
UseTagsHA and MS2 dimerExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE
Plasmid#104495PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switchDepositorHas ServiceAAV PHP.eB, AAV Retrograde, and AAV1InsertjGCaMP7s
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCAGAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H187
Plasmid#170348PurposemT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 MCS-BirA(R118G)-HA
Plasmid#36047PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tagDepositorHas ServiceCloning Grade DNAInsertBirA(R118G)-HA
UseTagsHAExpressionMammalianMutationR118G (highly promiscuous form)PromoterAvailable sinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.10
Plasmid#102919Purposeexpresses ABE7.10 in mammalian cellsDepositorInsertABE7.10
UseTagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-deLACCO1-NGR
Plasmid#208024PurposeControl biosensor for extracellular L-lactateDepositorInsertdeLACCO1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMX-Baf155
Plasmid#25856DepositorInsertBrg1 associated factor 155 (Smarcc1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP670-tubulin
Plasmid#136573PurposeHuman alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertemiRFP670-tubulin
UseTagsemiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Ef1a:PE2-P2A-GFP
Plasmid#184445PurposeSingle EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and Ef1AAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-HP1beta
Plasmid#17651DepositorInsertHP1 beta (CBX1 Human)
UseTagsGFP and HisExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_NES_C1
Plasmid#85060PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-RhoBAST
Plasmid#196341PurposeOverexpression of circular RhoBASTDepositorInsertTornado-RhoBAST
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)
Plasmid#197500PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…PromoterCMV and T7Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MYC-Sumo3Q89P
Plasmid#48963PurposeExpresses MYC-Sumo3Q90P mutant in mammalian cellsDepositorInsertSUMO3 (SUMO3 Human)
UseTagsMYC (EQKLISEED)ExpressionMammalianMutationMutated Q89PPromoterT7Available sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
Beta-arrestin2 GFP WT
Plasmid#35411DepositorInsertβarrestin2 (Arrb2 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CLASP2 512-650 9xS/A
Plasmid#24410DepositorInsertCLASP2 (512-650) 9xS/A (CLASP2 Human)
UseTagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant that c…PromoterAvailable sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF1972
Plasmid#143987PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTBX2 (TBX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-ADAR2
Plasmid#117929PurposeADAR2 expression plasmid.DepositorInsertADAR2 (ADARB1 Human)
UseTagsmyc-GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Mm.cargo(Cre)
Plasmid#174862PurposeExpresses mouse Peg10 cargoRNA encoding Cre recombinaseDepositorInsertCre flanked by MmPeg10 UTRs
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q, R261Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4 DD
Plasmid#26999DepositorInsertNEDD4 (NEDD4 Human)
UseTagsHAExpressionMammalianMutationC867APromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmVenus(L68V)-mTurquoise2
Plasmid#60493PurposeProduces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)DepositorInsertSYFP2-mTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-HK2-3xFLAG
Plasmid#163451Purposelentiviral overexpression vector for human HK2DepositorInsertHK2 (HK2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES-EGFP-puro
Plasmid#45567DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsIRES-EGFP-PuroExpressionMammalianMutationPromoterPhcmv (strong human cytomegalovirus immediate ear…Available sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA
Plasmid#158988PurposeVector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neuronsDepositorInsertdSaCas9-KRAB
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterpMecp2Available sinceAug. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-hM3D(Gq)
Plasmid#45547PurposeExpresses hM3D (Gq) neuronal activatorDepositorInserthM3D (Gq)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBI-p53 R175H/EGFP
Plasmid#16544DepositorInsertp53 (TP53 Human)
UseTagsExpressionMammalianMutationR175HPromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV IGF1-FLAG
Plasmid#175170PurposeLentiviral expression of human Igf1-FLAGDepositorInsertIgf1 (IGF1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac dCas9-VPR
Plasmid#191269PurposepiggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFPDepositorInsertsVPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO
UseCRISPRTagsHA and tagBFPExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-flex-iGluSnFR4f-NGR-WPRE
Plasmid#234442PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4f-NGR
UseAAV and Cre/LoxTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianMutationPromoterSynapsinAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
FoxO1-ADA-GFP
Plasmid#35640DepositorInsertFoxO1-ADA-GFP (Foxo1 Mouse)
UseTagsFlag, GFP, and MycExpressionMammalianMutationT24A, S253D and S316A mutationsPromoterCMVAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
PRS_8-eGFP
Plasmid#192589PurposeAAV-vector for fluorescent labeling of noradrenergic neuronsDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterPRSX8Available sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mStayGold
Plasmid#227250PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
UBC plasmid
Plasmid#169746PurposeUnregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.DepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-Rab11-WT
Plasmid#101047PurposeExpresses HA-tagged human Rab11-WT in mammalian cellsDepositorInsertRAB11 (RAB11A Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-LaminB1-10
Plasmid#57141PurposeLocalization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmPA-GFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
LCV2 STING KO
Plasmid#217445PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human STINGDepositorInsertsgRNA targeting STING (STING1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP8A1-HA
Plasmid#209208PurposeMammalian expression of ATP8A1DepositorInsertATP8A1 (ATP8A1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6f.WPRE.SV40
Plasmid#100837PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only
