78,292 results

1. pCMV(CAT)T7-SB100

   Plasmid

   #34879

   Depositor

   Zsuzsanna Izsvak

   Article

   Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

   Insert

   SB100X transposase
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF6-Acly

   Plasmid

   #70765

   Purpose

   Expresses mouse ATP-citrate lyase in mammalian cells

   Depositor

   Kathryn Wellen

   Article

   Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

   Insert

   ATP Citrate Lyase (Acly Mouse)
   

   Tags

   myc-epitope

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-tet-iCas9-BFP2

   Plasmid

   #125519

   Purpose

   all-in-one-inducible Cas9

   Depositor

   Jason Moffat

   Article

   Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

   Insert

   AAVS1-TET-ON 3G-SpCas9-P2A-BFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pV238-vPE

   Plasmid

   #225258

   Purpose

   Mammalian expression of vPE

   Depositor

   Vikash Chauhan, Robert Langer, Phil Sharp

   Article

   Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

   Insert

   vPE
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   R221K K848A H982A N1317R

   Promoter

   CMV

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. M51 Super 8x FOPFlash (TOPFlash mutant)

   Plasmid

   #12457

   Purpose

   Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   mutant TCF/LEF binding sites (Ctnnb1 )
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   mutant TCF/LEF binding sites

   Available Since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. c-Flag pcDNA3

   Plasmid

   #20011

   Purpose

   This pcDNA3 plasmid contains a new poly-linker and single C-terminal flag-tag sequence for expression in mammalian cells.

   Depositor

   Stephen Smale

   Article

   Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCXLE-hOCT3/4-shp53-F

   Plasmid

   #27077

   Purpose

   Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   OCT3/4 (POU5F1 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCXLE-hUL

   Plasmid

   #27080

   Purpose

   Integration-free (episomal) expression of human L-MYC and LIN28

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   L-MYC (LIN28A, MYCL Human)
   

   Expression

   Mammalian

   Available Since

   March 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-3'TwStrep

   Plasmid

   #208618

   Purpose

   Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   Twin Strep

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 His-V5 Myc

    Plasmid

    #176049

    Purpose

    Expresses Myc tagged with His and V5 in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Tags

    6xHis and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF2497

    Plasmid

    #142115

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ASCL1 (ASCL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCXLE-hSK

    Plasmid

    #27078

    Purpose

    Integration-free (episomal) expression of human SOX2 and KLF4

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    SOX2, KLF4 (KLF4, SOX2 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. YAP/TAZ-TRE-mStrawberry reporter

    Plasmid

    #158682

    Purpose

    This plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-tevopreq1-GG-acceptor

    Plasmid

    #174038

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. V5-TurboID-NES_pCDNA3

    Plasmid

    #107169

    Purpose

    expresses V5-tagged TurboID in the mammalian cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. UIM-ECFP

    Plasmid

    #21067

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    UIM (Epn1 Rat)
    

    Tags

    ECFP

    Expression

    Mammalian

    Available Since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pCAG-Cre

    Plasmid

    #13775

    Purpose

    Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9n(BB)-2A-Puro (PX462) V2.0

    Plasmid

    #62987

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. HIF-TRE-mStrawberry reporter

    Plasmid

    #158681

    Purpose

    This plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding element promoter driving the expression of mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-TALEN-L

    Plasmid

    #59025

    Purpose

    TALEN designed to target the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    AAVS1-TALEN-L
    

    Use

    AAV and TALEN

    Tags

    3xFlag and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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