Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,559 results for: Chia

Showing: 5001 - 5050 of 5559 results

  2. Burden Monitor lambda version

    Plasmid
    #66073
    Purpose
    The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:
    Insert
    GFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pJB02

    Plasmid
    #111959
    Purpose
    Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments
    Depositor
    Matthew Good
    Article
    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259
    Inserts
    mEGFP
    eDHFR
    Use
    Tags
    DHFR
    Expression
    Bacterial
    Mutation
    eDHFR;residues 2-159 and enhanced GFP with monome…
    Promoter
    T7
    Available since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111959-plasmid-map-sequence-id-219913

    Enlarge

  5. pTS2340_Tier3(SB)-iRFP_Puro

    Plasmid
    #169638
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven iRFP670 and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169638-plasmid-map-sequence-id-364985

    Enlarge

  7. ins:CFP-NTR

    Plasmid
    #66593
    Purpose
    Specific and conditional cell ablation of beta cells in the zebrafish pancreas
    Depositor
    Didier Stainier
    Article
    Curado et al Dev Dyn. 2007 Apr . 236(4):1025-35.
    Insert
    Insulin promotor driving CFP-nitroreductase
    Use
    Zebrafish expression
    Tags
    CFP fused with N-terminus of nitroreductase
    Expression
    Mutation
    Promoter
    Zebrafish Insulin promoter (approximately 1.2kb)
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pBRα-σ70 D581G

    Plasmid
    #53732
    Purpose
    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The sigma 70 moiety carries the D581G substitution
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pC3.1_CAG_oROS-Gr

    Plasmid
    #216113
    Purpose
    Expresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.01.31.578117
    Insert
    CAG_oROS-Gr
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216113-plasmid-map-sequence-id-430076

    Enlarge

  13. pMS17

    Plasmid
    #127088
    Purpose
    Integrating vector containing a tetracycline inducible promoter for use in Streptomyces sp.
    Depositor
    Maggie Smith
    Article
    Rodriguez-Garcia et al Nucleic Acids Res. 2005 May 25;33(9):e87
    Inserts
    tcp830
    TetRiS
    terminator mmrt
    Terminator fdt
    Use
    Streptomyce integration vector derived from phic3…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    127088-plasmid-map-sequence-id-249126

    Enlarge

  17. pET19b-EcMscL-TEV-mEGFP

    Plasmid
    #165097
    Purpose
    Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.
    Depositor
    Neha Kamat
    Article
    Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p
    Insert
    E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
    Use
    Synthetic Biology
    Tags
    monomeric Enhanced Green Fluorescent Protein
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    165097-plasmid-map-sequence-id-324525

    Enlarge

  20. pRelA'

    Plasmid
    #175595
    Purpose
    Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175595-plasmid-map-sequence-id-348284

    Enlarge

  22. pLAREG

    Plasmid
    #73044
    Purpose
    Inducible expression of EGFP in yeast. Has dual-mode promoter with activation and repression capability for tuning gene expression in yeast.
    Depositor
    David McMillen
    Article
    Mazumder et al Nucleic Acids Res. 2014 Aug;42(14):9514-
    Inserts
    EGFP
    LacI
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 promoter and hybrid dual-mode promoter-see c…
    Available since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73044-plasmid-map-sequence-id-136659

    Enlarge

  26. pKSJ376

    Plasmid
    #103065
    Purpose
    Expresses colicin E1 with R domain deletion and ImmE1
    Depositor
    Alan Finkelstein, Karen Jakes
    Article
    Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0
    Inserts
    colicin E1 immunity protein (pColE1_26, imm E. coli)
    cea/colicin E1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    R (receptor-binding domain) deletion in colicin E…
    Promoter
    T7 lac (from pET) and T7 lac (from pET52-b)
    Available since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    103065-plasmid-map-sequence-id-200637

    Enlarge

  29. Dead Streptavidin-SpyTag

    Plasmid
    #59548
    Purpose
    pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead Streptavidin-SpyTag
    Use
    Tags
    SpyTag
    Expression
    Bacterial
    Mutation
    The construct lacks final K of SpyTag
    Promoter
    T7
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. pET22b_EcSSB_1

    Plasmid
    #78203
    Purpose
    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
    Depositor
    Martin Webb
    Article
    Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1
    Insert
    ssb (G26C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G26C (please see depositor comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78203-plasmid-map-sequence-id-151729

    Enlarge

  32. pET28a-B4E

    Plasmid
    #162067
    Purpose
    For expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E
    Depositor
    Karen Polizzi
    Article
    Moya-Ramirez et al Nucleic Acids Res. 2020 Dec 16;48(22):e1
    Insert
    ampR-eIF4E fusion protein
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    eIF4E incorporating the K119A mutation, which is …
    Promoter
    T7
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    162067-plasmid-map-sequence-id-325536

    Enlarge

  35. pET22b_EcSSB_2

    Plasmid
    #78204
    Purpose
    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor
    Depositor
    Martin Webb
    Article
    Fili et al Nucleic Acids Res. 2010 Jul;38(13):4448-
    Insert
    ssb (W88C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    W88C (please see depositor comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78204-plasmid-map-sequence-id-151737

    Enlarge

  36. pACM4-ACA

    Plasmid
    #49809
    Purpose
    Encodes fabD, accA, accB, accC, accD in pseudo-operon configuration.
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    ACP transacylase
    Acetyl-CoA carboxylase
    acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Acetyl-CoA carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Silent site-directed mutagenesis to remove SalI s…
    Promoter
    T7
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49809-plasmid-map-sequence-id-74624

    Enlarge

  40. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

    Enlarge

  42. pBAD24-rnc-sfGFP

    Plasmid
    #51560
    Purpose
    Gene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).
    Depositor
    Francisco Malagon, Sankar Adhya
    Article
    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261
    Insert
    rnc (rnc E. coli)
    Use
    Tags
    GLESTCRHASLAVLADERRFSA and superfolder GFP
    Expression
    Bacterial
    Mutation
    Contains additonal aa at the end of the sfGFP in …
    Promoter
    Available since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    51560-plasmid-map-sequence-id-78838

    Enlarge

  48. pJL1-MBP-4xDQNAT

    Plasmid
    #128390
    Purpose
    In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Maltose binding protein
    Use
    Tags
    4xDQNAT glycosylation tag and 6xHis tag
    Expression
    Bacterial
    Mutation
    A302T- please see depositor comment
    Promoter
    T7
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128390-plasmid-map-sequence-id-260183

    Enlarge

Showing: 5001 - 5050 of 5559 results