8,631 results

1. pGEX-2T-Rac1-wt

   Plasmid

   #12977

   Depositor

   Gary Bokoch

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

2. HIVGKO

   Plasmid

   #112234

   Purpose

   HIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1a

   Depositor

   Eric Verdin

   Article

   Battivelli et al Elife. 2018 May 1;7. pii: 34655. doi: 10

   Insert

   csGFP-EF1a-mKO2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Flag-HA-USP1

   Plasmid

   #22596

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP1 (USP1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. anxA2-GFP

   Plasmid

   #107196

   Purpose

   Expresses human annexin A2-GFP fusion protein for live cell imaging

   Depositor

   Volker Gerke, Ursula Rescher

   Article

   Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

   Insert

   annexin A2 (ANXA2 Human)
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Ala residue 65 in human sequence replaced by Glu …

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 3xERRE-luciferase

   Plasmid

   #37851

   Depositor

   Rebecca Riggins

   Article

   Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

   Insert

   3 copies of ERRE upstream of luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. shh10

   Plasmid

   #64867

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

   Depositor

   John Flannery, David Schaffer

   Article

   Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

   Insert

   shh10 cap
   

   Use

   AAV

   Tags

   Expression

   Mutation

   I319V, N451D, D532N

   Promoter

   Available since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AIMTOR T757

   Plasmid

   #140828

   Purpose

   AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells

   Depositor

   Anne Bonnieu

   Article

   Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

   Insert

   AIMTOR T757 (ULK1 Human)
   

   Use

   Tags

   NES Nuclear export signal

   Expression

   Mammalian

   Mutation

   The insert comprises only a small sequence of hum…

   Promoter

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEJS614_pTetR-P2A-BFPnls/sgNS

   Plasmid

   #108650

   Purpose

   Non-specific Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Non-specific sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

   Plasmid

   #41863

   Purpose

   This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS6-YFP-SMASh

    Plasmid

    #68853

    Purpose

    Expresses SMASh tag fused to YFP at C terminus in mammalian cells

    Depositor

    Michael Lin

    Article

    Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

    Insert

    YFP-SMASh
    

    Use

    Tags

    SMASh tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGS SARS-CoV-2 BA.4/5 Spike

    Plasmid

    #186031

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.4/5 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

    Insert

    SARS-CoV-2 BA.4/5 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    BA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pLenti CMVtight Hygro DEST (w769-1)

    Plasmid

    #26433

    Depositor

    Eric Campeau

    Type

    Empty backbone

    Use

    Lentiviral; Tet-on advanced destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-EZH2

    Plasmid

    #28060

    Depositor

    Mien-Chie Hung

    Article

    Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mOrange-N1

    Plasmid

    #54499

    Purpose

    Localization: N1 Cloning Vector, Excitation: 548, Emission: 562

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mOrange

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXs-IP spGFP-ERGIC53

    Plasmid

    #38270

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    ERGIC-53 (LMAN1 Human)
    

    Use

    Retroviral

    Tags

    EGFP with Calreticulin signal peptide at N-termin…

    Expression

    Mammalian

    Mutation

    deleted signal peptide (1-30 amino acids)

    Promoter

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mVenus-pBAD

    Plasmid

    #54845

    Purpose

    Localization: Protein Expression Vector , Excitation: 515, Emission: 528

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

    Type

    Empty backbone

    Use

    Tags

    6xHis and mVenus

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pCMV-Myc-GFP

    Plasmid

    #83375

    Purpose

    Expression of Myc tagged GFP

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    Myc-GFP
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pK036.TRE-Flpe-WPRE (Supernova)

    Plasmid

    #85007

    Purpose

    For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    NLS-FLPe-WPRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4TO-mito-mCherry-10xGCN4_v4

    Plasmid

    #60914

    Purpose

    A fusion protein of the SunTag10xGCN4 v4 peptide array to a mitochondrial targeting domain and mCherry

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    mito-mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti CMV p16 Neo (w111-1)

    Plasmid

    #22260

    Depositor

    Eric Campeau

    Insert

    cyclin-dependent kinase inhibitor 2A (CDKN2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKH3-human RSK1

    Plasmid

    #13841

    Depositor

    John Blenis

    Article

    Richards et al Mol Cell Biol. 2001 Nov . 21(21):7470-80

    Insert

    RSK1 (RPS6KA1 Human)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-Myc3-CAND1

    Plasmid

    #19948

    Depositor

    Yue Xiong

    Article

    Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

    Insert

    CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

23. LBR pEGFP-N2 (646)

    Plasmid

    #61996

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    LBR (LBR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3

    Plasmid

    #112008

    Purpose

    For cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Insert

    axon-GCaMP6s-P2A-mRuby3
    

    Use

    AAV and Cre/Lox

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGL3B-1081

    Plasmid

    #18759

    Depositor

    Clark Distelhorst

    Article

    Wang et al Oncogene. 2006 Mar 23. 25(13):1903-13.

    Insert

    thioredoxin-interacting protein promoter (Txnip Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

26. mCherry-Rab4a-7

    Plasmid

    #55125

    Purpose

    Localization: Ras GTPase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGOAL19

    Plasmid

    #20190

    Depositor

    Tanya Parish

    Article

    Parish et al Microbiology. 2000 Aug . 146 ( Pt 8)():1

    Type

    Empty backbone

    Use

    Marker cassette

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Flag-cIAP2/pRK5

    Plasmid

    #27973

    Depositor

    Xiaolu Yang

    Article

    Hu et al J Clin Invest. 2006 Jan . 116(1):174-81.

    Insert

    CIAP2 (BIRC3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-TIWB-myrmScarlet-I-P2A-post-eGRASP

    Plasmid

    #111584

    Purpose

    An AAV vector that expresses myristoylated mScarlet-I and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    myrmScarlet-I-P2A-post-eGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetO

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pmCherry-C1-RanQ69L

    Plasmid

    #30309

    Depositor

    Jay Brenman

    Article

    Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

    Insert

    RanQ69L (RAN N/A)
    

    Use

    Tags

    FLAG-Cherry

    Expression

    Mammalian

    Mutation

    Glutamine 69 is changed to Leucine

    Promoter

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pYPQ230 (LbCpf1)

    Plasmid

    #86210

    Purpose

    LbCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    LbCpf1
    

    Use

    CRISPR; Gateway compatible lbcpf1 entry clone

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-NHERF1-FL

    Plasmid

    #28291

    Depositor

    Maria-Magdalena Georgescu

    Article

    Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

    Insert

    NHERF1 (NHERF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-GST

    Plasmid

    #42049

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    GST
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Green Glifon50

    Plasmid

    #126206

    Purpose

    green fluorescent glucose sensor for mammalian cells

    Depositor

    Tetsuya Kitaguchi

    Article

    Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

    Insert

    Green Glifon50
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-dLight1.1

    Plasmid

    #111053

    Purpose

    Expresses dLight1.1 in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.1
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pZac2.1 gfaABC1D-cyto-GCaMP6f

    Plasmid

    #52925

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Baljit Khakh

    Article

    Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1

    Has Service

    AAV5

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCI-neo FlucSM EGFP

    Plasmid

    #90171

    Purpose

    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutation

    Depositor

    Franz-Ulrich Hartl

    Article

    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

    Insert

    FLuc EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R188Q in FLuc

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GST-HA-GFP11-N1

    Plasmid

    #91953

    Purpose

    Expresses GST-HA-GFP11 in mammalian cells

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Insert

    GST-HA-GFP11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJFRC-MUH

    Plasmid

    #26213

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV6 BDNF

    Plasmid

    #39857

    Depositor

    Yves-Alain Barde

    Article

    Hofer et al EMBO J. 1990 Aug;9(8):2459-64.

    Insert

    BDNF (Bdnf Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3-cdk5GFP

    Plasmid

    #1346

    Depositor

    Li-Huei Tsai

    Insert

    CDK5 (CDK5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42229

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pREDTKI

    Plasmid

    #51628

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pDONR223 SARS-CoV-2 NSP16

    Plasmid

    #141269

    Purpose

    Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. Su9-EGFP

    Plasmid

    #23214

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Subunit 9 of the F0-ATPase
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains AA 1-69 of subunit 9

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBABEpuro-ERBB2

    Plasmid

    #40978

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pZS*12-MS2-GFP

    Plasmid

    #26245

    Depositor

    Philippe Cluzel

    Article

    Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

    Insert

    MS2-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    five amino acid linker (Ser, Gly, Gly, Gly, Gly) …

    Promoter

    Available since

    Nov. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pJMP2

    Plasmid

    #79874

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into amyE

    Depositor

    Jason Peters, Stanley Qi, Carol Gross

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    veg

    Available since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. AAV_NLS-dSaCas9-NLS-VPR

    Plasmid

    #68495

    Purpose

    AAV vector containing nuclease null SaCas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    dSaCas9
    

    Use

    AAV

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pGL3 NSE promoter

    Plasmid

    #11606

    Depositor

    Rosalyn Adam

    Article

    Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

    Insert

    NSE promoter (ENO2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge