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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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8,631 results

Showing: 5401 - 5450 of 8631 results

  3. pCPRDEnv

    Plasmid
    #1732
    Purpose
    This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab
    Depositor
    Garry Nolan
    Insert
    Gag-Pol and Rev
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 24, 2005
    Availability
    Academic Institutions and Nonprofits only
    1732-plasmid-map-sequence-id-326991

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  15. pRK5-myc-RhoA-Q63L

    Plasmid
    #12964
    Depositor
    Gary Bokoch
    Insert
    RhoA constitutively active (RHOA Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Q63L Constitutively active
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    12964-plasmid-map-sequence-id-139200

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  16. pSBtet-Neo

    Plasmid
    #60509
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60509-plasmid-map-sequence-id-97538

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  25. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid
    #74707
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence
    Depositor
    Eugene Yeo
    Article
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Insert
    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    74707-plasmid-map-sequence-id-152737

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  29. pcDNA3-SARS-CoV-2-S-RBD-Fc

    Plasmid
    #141183
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
    Use
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141183-plasmid-map-sequence-id-288058

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  33. MIGR-mFoxP3

    Plasmid
    #24067
    Depositor
    Dan Littman
    Insert
    Foxp3 (Foxp3 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24067-plasmid-map-sequence-id-473505

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  37. pLenti6.2-ccdB-Nanoluc

    Plasmid
    #87075
    Purpose
    Lentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87075-plasmid-map-sequence-id-215540

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  43. AAV phSyn1(S)-DreO-bGHpA

    Plasmid
    #50363
    Purpose
    Can be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Has Service
    AAV Retrograde and AAV5
    Insert
    DreO recombinase
    Use
    AAV
    Tags
    none
    Expression
    Mutation
    Sequence optimized for expression in mammalian ce…
    Promoter
    hSyn1
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50363-plasmid-map-sequence-id-308988

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  46. pRK5-myc-RhoA-T19N

    Plasmid
    #12963
    Depositor
    Gary Bokoch
    Insert
    RhoA DN (RHOA Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    T19N Dominant negative
    Promoter
    Available since
    March 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    12963-plasmid-map-sequence-id-223086

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  47. pWS158

    Plasmid
    #90517
    Purpose
    Cas9 gap repair vector - URA3
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90517-plasmid-map-sequence-id-287206

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  50. MSP1830

    Plasmid
    #70708
    Purpose
    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Insert
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    CAG
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    70708-plasmid-map-sequence-id-364684

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Showing: 5401 - 5450 of 8631 results