166,169 results

1. 6379 H10-K218T

   Plasmid

   #1250

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1998 Jul 10;94(1):73-82.

   Insert

   Hsp104 (K218T) (HSP104 Budding Yeast)
   

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   K218T

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

2. 6380 H10-K620T

   Plasmid

   #1251

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1998 Jul 10;94(1):73-82.

   Insert

   Hsp104 (K620T) (HSP104 Budding Yeast)
   

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   K620T

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pJL056

   Plasmid

   #177168

   Purpose

   Expresses wildtype MRE11 in human cells

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Insert

   MRE11 (MRE11 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tn7 helper plasmid

   Plasmid

   #141161

   Purpose

   Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

   Depositor

   Shimon Bershtein

   Article

   Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

   Insert

   Tn7 transposon machinery
   

   Expression

   Bacterial

   Promoter

   Arabinose-inducible pBAD

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-nlsABACUS2-400n

   Plasmid

   #203726

   Purpose

   Gateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acid

   Depositor

   Alexander Jones

   Article

   Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

   Insert

   nlsABACUS2-400n
   

   Use

   Synthetic Biology

   Tags

   Myc

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDESTmycYBX1

   Plasmid

   #19878

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   Y box binding protein 1 (YBX1 Human)
   

   Tags

   MYC

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_Pax6-RE_tBFP_PGK_mCitrine

   Plasmid

   #188388

   Purpose

   Lentiviral vector - tBFP reporter for Pax6-based SNIPRs with a constitutive mCitrine

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   Pax6-RE_tBFP_PGK_mCitrine
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-Cre

   Plasmid

   #198712

   Purpose

   For lentiviral expression of Cre recombinase in mammalian cells.

   Depositor

   Tyler Jacks

   Article

   Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

   Insert

   Pgk-Cre
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIV-H2B-eGFP

   Plasmid

   #91776

   Purpose

   Expresses H2B protein tagged with eGFP

   Depositor

   Maria Pia Cosma

   Article

   Sottile et al Sci Rep. 2016 Nov 9;6:36863. doi: 10.103

   Insert

   Human Histone H2B / eGFP (H2BC11 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-Oct3/4

    Plasmid

    #13366

    Purpose

    Retroviral expression of mouse Oct3/4

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-Sox2

    Plasmid

    #101761

    Purpose

    Sox2 promoter reporter

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

    Insert

    SRY-box 2 promoter (SOX2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXJ-Myc-IRF2

    Plasmid

    #232642

    Purpose

    transient expresses IRF2 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    IRF2 (IRF2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-D1R

    Plasmid

    #89160

    Purpose

    Express vaccinia virus D1R in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D1R
    

    Expression

    Mammalian

    Promoter

    CAG promotor

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57-MCS1-P2A-MCS2 (Hygro)

    Plasmid

    #80922

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

    Plasmid

    #209651

    Purpose

    Expression of iATPSnFR2, high affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.S29W.HaloTag
    

    Use

    AAV

    Mutation

    S29W.A95K

    Promoter

    CAG

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRIPZ shNS

    Plasmid

    #127696

    Purpose

    Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shNS
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p6599 MSCV-IP N-HAonly FOSL1

    Plasmid

    #34897

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    FOSL1 (FOSL1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUGI-NLS

    Plasmid

    #101091

    Purpose

    Expresses UGI in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

    Insert

    UGI
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-PspCas13b-gRNA-Actb1216

    Plasmid

    #155368

    Purpose

    PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    PspCas13b gRNA targeting Actb 1216
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. p406TEF1

    Plasmid

    #15976

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    TEF1 promoter is between SacI and XbaI restrictio…

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDEST17-UnaG

    Plasmid

    #203485

    Purpose

    For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    UnaG
    

    Expression

    Bacterial and Plant

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. hrGFP-Keap1

    Plasmid

    #28025

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    Keap1 (KEAP1 Human)
    

    Tags

    hrGFP

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. 3xnls-mNeonGreen

    Plasmid

    #98875

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    3xNLS
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pDAC435

    Plasmid

    #195238

    Purpose

    Mammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP
    

    Expression

    Mammalian

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLIB

    Plasmid

    #80610

    Purpose

    pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRH030

    Plasmid

    #159633

    Purpose

    CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

    Depositor

    Robert Hertel

    Article

    Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

    Type

    Empty backbone

    Use

    CRISPR; Shuttle vector

    Promoter

    Constitutive tracrRNA

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-D12L

    Plasmid

    #89161

    Purpose

    Express vaccinia virus D12L in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D12L
    

    Expression

    Mammalian

    Promoter

    CAG promotor

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RXFP3-Tango

    Plasmid

    #66494

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    RXFP3 (RXFP3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pCMV CEPIA3mt

    Plasmid

    #58219

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA3mt
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V E334D M339L

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFlp-Tc

    Plasmid

    #222336

    Purpose

    Expresses Flp and tetracycline to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB )
    flp (flp )
    cI (cI )
    

    Promoter

    cl, flp, and sacB

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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31. FTL

    Plasmid

    #100750

    Purpose

    Part of FerriTag expression system. Untagged Ferritin light chain in mammalian expression vector.

    Depositor

    Stephen Royle

    Article

    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

    Insert

    FTL (FTL Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-ClpX

    Plasmid

    #234733

    Purpose

    Human-codon optimized E. coli ClpX in mammalian cells

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    ClpX
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PB-TO-hNGN2-BSD-mApple

    Plasmid

    #182308

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    hNGN2 (NEUROG2 Human)
    bsd-T2A-mycNLS-mApple
    

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLVX NPC1-FLAG-TiD

    Plasmid

    #199748

    Purpose

    Lentiviral construct to stably express NPC1 TurboID

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    NPC1 (NPC1 Human)
    

    Tags

    Flag and TurboID

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTriEx-NTOM20-LOV2

    Plasmid

    #81009

    Purpose

    Expression of LOV2 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2
    

    Tags

    NTOM20

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV, p10

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUCmini-iCAP-PHP.eC

    Plasmid

    #201903

    Purpose

    non-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Shay et al Sci Adv. 2023 Apr 21;9(16):eadg6618. doi

    Insert

    Synthetic construct isolate AAV-PHP.eC VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLexm

    Plasmid

    #99844

    Purpose

    mammalian expression

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBud-EGFP

    Plasmid

    #23027

    Depositor

    Andrea LeBlanc

    Article

    Jodoin et al PLoS One. 2009 . 4(8):e6647.

    Insert

    EGFP (eGFP )
    

    Expression

    Bacterial and Mammalian

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mCherry-Sequestosome1-N-18

    Plasmid

    #55132

    Purpose

    Localization: Membrane Scaffold, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Sequestosome1 (SQSTM1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

40. Ezh2[FL]-dCas9

    Plasmid

    #100086

    Purpose

    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    14056 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Ezh2[FL]-dCas9-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge