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8,789 results

Showing: 601 - 640 of 8789 results

  1. pCMV-PE6b

    Plasmid
    #207852
    Purpose
    Mammalian expression of PE6b prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6b
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Promoter
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207852-plasmid-map-sequence-id-408170

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  5. pHAGE-E-cadherin-RFP

    Plasmid
    #79603
    Purpose
    pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system
    Depositor
    Jeffrey Rosen
    Article
    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.
    Insert
    CDH1 promoter region (CDH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    79603-plasmid-map-sequence-id-152509

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  6. pCI-VSVG

    Plasmid
    #1733
    Purpose
    VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab
    Depositor
    Garry Nolan
    Insert
    VSV-G Envelope
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    1733-plasmid-map-sequence-id-334

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  7. FUGW-d2GFP-ZEB1 3'UTR

    Plasmid
    #79601
    Purpose
    FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.
    Depositor
    Jeffrey Rosen
    Article
    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.
    Insert
    d2GFP-Zeb1 3'UTR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    79601-plasmid-map-sequence-id-398021

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  8. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Irene Coin
    Article
    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464
    Inserts
    Humanized MbPylRS
    tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105830-plasmid-map-sequence-id-466939

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  9. CD63-pEGFP C2

    Plasmid
    #62964
    Purpose
    full length human CD63 cloned into pEGFP C2
    Depositor
    Paul Luzio
    Insert
    CD63 molecule (CD63 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CD63 has 1 silent mutation compared to the coding…
    Promoter
    CMV
    Available Since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62964-plasmid-map-sequence-id-207850

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  10. pX330-BbsI-PITCh

    Plasmid
    #127875
    Purpose
    For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly
    Depositor
    Peter Kaiser
    Article
    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127875-plasmid-map-sequence-id-482883

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  18. mEGFP-C1

    Plasmid
    #54759
    Purpose
    Localization: C1 Cloning Vector, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54759-plasmid-map-sequence-id-320190

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  26. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available Since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  28. pTRKH2

    Plasmid
    #71312
    Purpose
    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.
    Depositor
    Rodolphe Barrangou, Todd Klaenhammer
    Article
    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.
    Type
    Empty backbone
    Use
    E. coli-gram positive bacteria shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    71312-plasmid-map-sequence-id-323567

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  32. pJR104

    Plasmid
    #187243
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187243-plasmid-map-sequence-id-374823

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  39. pBBR1MCS-2

    Plasmid
    #85168
    Purpose
    Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.
    Depositor
    Kenneth Peterson
    Article
    Kovach et al Gene. 1995 Dec 1;166(1):175-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive (derepressed P-lac)
    Available Since
    Nov. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    85168-plasmid-map-sequence-id-372970

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  40. psPAX2-D64V

    Plasmid
    #63586
    Purpose
    psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.
    Depositor
    Andrew Scharenberg, David Rawlings
    Article
    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi
    Insert
    Packaging plasmid for integrase deficient lentiviral vectors
    Use
    Integrase deficient lentiviral packaging vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    April 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    63586-plasmid-map-sequence-id-464218

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Showing: 601 - 640 of 8789 results