19,190 results
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CROPseq-Guide-EFS-SpCas9-P2A-EGFP
Plasmid#99248Purposesingle-vector system with EGFP reporter for use in CROPseq knockout screensDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsEGFPExpressionMammalianMutationPromoterEFSAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a SpyCatcher-SnoopCatcher
Plasmid#72324PurposeBacterial expression of SpyCatcher linked to SnoopCatcher.DepositorInsertpET28a SpyCatcher-SnoopCatcher
UseTagsN-terminal His6 tagExpressionBacterialMutation35 amino acids at the N-term of SpyCatcher have b…PromoterAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
XBP-1 2: pFLAG.XBP1p.CMV2
Plasmid#21833DepositorInsertXBP-1 processed/spliced (Xbp1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHIV_pTp66p51
Plasmid#78233PurposeExpresses HIV reverse transcriptaseDepositorInsertsp66
p51
LacI
UseTagsExpressionBacterialMutationPromoterConst. Promoter (From DNA2.0 Vec) and pTac Minima…Available sinceAug. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIU211
Plasmid#44036DepositorInsertAGA1
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
E1b-GFP-Tol2
Plasmid#37845DepositorTypeEmpty backboneUseZebrafish enhancer assay vectorTagsExpressionMutationPromoterE1bAvailable sinceOct. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
hAAVS1 1L TALEN
Plasmid#35431PurposeTALEN designed to target the human AAVS1 locusDepositorInserthAAVS1 1L TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pinducer 20 DN-KASHΔPPPL
Plasmid#129280PurposeTet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPLDepositorInsertSYNE1 (SYNE1 Human)
UseLentiviralTagsmcherryExpressionMutationthe last 4 amino acids (PPPL) of the KASH domain …PromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-RBBP4
Plasmid#20715DepositorInsertretinoblastoma binding protein 4 (RBBP4 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHDM-Tat1b
Plasmid#164442PurposeEncodes HIV-1 Tat accessory protein for pseudovirus productionDepositorInsertHIV-1 Tat
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP-5UAS DsRedExpress
Plasmid#74317PurposeA fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafishDepositorInsertssynaptophysin
DsRedExpress
UseTagsEGFPExpressionMutationPromoter5UASAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAIP hIL4 co
Plasmid#74169PurposeLentiviral vector for expression of human codon optimized IL4 with puromycin resistance.DepositorInsertIL4 (IL4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-Rab11-WT
Plasmid#101047PurposeExpresses HA-tagged human Rab11-WT in mammalian cellsDepositorInsertRAB11 (RAB11A Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Twitch-2B pRSETB
Plasmid#48203PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-2B
UseTags6xHIS, cpVenusCD, and mCerulean3ExpressionBacterialMutationPromoterT7Available sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-GFP
Plasmid#40592DepositorInsertGFP-S65T
UseMouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionBacterial and MammalianMutationConstruct contains a mutated, humanized hGFP(S65T…PromoterhGFAP promoterAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-nucGFP
Plasmid#140190PurposeOverexpression of GFP with a nuclear localization sequence, under the human synapsin promoterDepositorInsertGFP-NLS
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro
Plasmid#141390PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-orf8 (ORF8 SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pST-KiT
Plasmid#44562Purposefor integrative inducible expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
HcKCR1_mCherry_pcDNA3.1
Plasmid#177336PurposeExpresses KCR1 in mammalian cellsDepositorInsertHcKCR1
UseTagsmCherryExpressionMammalianMutationmammalian codon-adaptedPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFC333
Plasmid#87844PurposeAMA1 plasmid with Aspergillus optimized Cas9 and ble selection markerDepositorInsertsCas9
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV JMJD3
Plasmid#21212DepositorInsertJMJD3 (KDM6B Human)
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceMay 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-flag-myr-Akt1-IRES-GFP
Plasmid#65063Purposemammalian retroviral expression plasmidDepositorInsertAkt1 (AKT1 Human)
UseTagsflag and myristoylation tagExpressionMammalianMutationPromoterAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ALK
Plasmid#23917DepositorInsertALK (ALK Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1 actin-3XNLS P2A mCherry
Plasmid#58475PurposeExpresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoterDepositorInsertsUseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE
Plasmid#162380PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterCAGAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
cdk1 FRET sensor (2327)
Plasmid#26064DepositorInsertCdk1 FRET Sensor
UseTagsCFP variant, FLAG, and YFP variantExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
Plasmid#61594PurposeA catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationD10A, N580APromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CIB1-mCerulean-MP
Plasmid#58366PurposeFor use in light-induced protein inactivation through the interaction with CRY2DepositorUseTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pG14-MS2-GFP
Plasmid#27117DepositorInsertMS2-binding protein
UseTagsGFP, HA, and NLSExpressionYeastMutationPromoterAvailable sinceDec. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP-TRIM33
Plasmid#65398PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertTRIM33 (TRIM33 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir
Plasmid#90009PurposeSplit-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBPDepositorInsertsFKBP-linker-Myc-NBirA*
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Desmoglein2-mCherry
Plasmid#36991DepositorInsertDsg2 (DSG2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
hIRE1a K599A
Plasmid#20745DepositorInsertIRE1 (ERN1 Human)
UseLentiviral; Destination vectorTagsExpressionMammalianMutationK599A, dominant negative kinase mutantPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET30-RT1306
Plasmid#131521PurposeExpresses engineered RT-1306 with His-tagDepositorInsertRT-1306
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPH6
Plasmid#40315DepositorInsertPeptide:N-glycosidase F
UseTags6xHis and OmpA3ExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
p-EGFP-C1-Flag-mFmr1(wt)
Plasmid#87929PurposeExpresses mouse FMRP-EGFP fusion, which can be phosphorylated on ser-499.DepositorInsertFmr1 (Fmr1 Mouse)
UseTagsFlag and eGFPExpressionMammalianMutationNonePromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHTNW
Plasmid#136403PurposeExpression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.DepositorTypeEmpty backboneUseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lys-eMagA-EGFP
Plasmid#162246PurposeBlue-light dependent heterodimerization systemDepositorInsertp18-eMagA-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-ArchT-WPREpA
Plasmid#84472PurposepAAV-RAM-ArchTDepositorInsertArchT
UseAAVTagsEGFPExpressionMutationPromoterTREAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Mito-Car-GECO
Plasmid#100765PurposeLentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imagingDepositorInsertLenti-Mito-Car-GECO
UseLentiviralTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationR-GECO1: E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMito.iGABASnFR
Plasmid#112175PurposeGABA SensorDepositorInsertiGABASnFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBG
Plasmid#22227PurposeGST fusion vectorDepositorTypeEmpty backboneUseTagsGSTExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEJS1099: Dual-sgRNA.Design 4
Plasmid#159537PurposeDelivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vectorDepositorInsertNme2Cas9 with two guide RNA cassettes
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianMutationPromoterU1aAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-mCherry-lenti
Plasmid#115796PurposeExpresses mCherry in human cells, contained in a lentiviral vector.DepositorInsertmCherry
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEF-1a promoterAvailable sinceOct. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB) (PX460)
Plasmid#48873PurposeCas9n (D10A nickase mutant) from S. pyogenesDepositorHas ServiceCloning Grade DNAInserthSpCas9n
UseCRISPRTags3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3E-2xFYVE_hrs
Plasmid#67676PurposeMultisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrsDepositorInsert2xFYVEhrs
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSGAb-spe
Plasmid#122000PurposeA sgRNA expression plasmid for genome editing in Acinetobacter baumanniiDepositorInsertnone
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMPRAv3:∆luc:∆xbaI
Plasmid#109035PurposeMPRA v3 empty backboneDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoternoneAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only
