Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

166,787 results

Showing: 1001 - 1050 of 166787 results

  6. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

    Plasmid
    #202569
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202569-plasmid-map-sequence-id-429223

    Enlarge

  7. pNL [NlucP/STAT5-RE/Hygro] Vector

    Plasmid
    #236819
    Purpose
    Express STAT5 response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    STAT5 (STAT5A Human)
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Mutation
    None
    Promoter
    minP
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236819-plasmid-map-sequence-id-471823

    Enlarge

  10. TFORF0070

    Plasmid
    #141505
    Purpose
    Lentiviral vector for overexpressing the CSDE1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CSDE1 (CSDE1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    141505-plasmid-map-sequence-id-454897

    Enlarge

  13. pCMV-T7-SpCas9(KWRQLC)-P2A-EGFP (RAS2864)

    Plasmid
    #223087
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KWRQLC)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(KWRQLC)=D1135K, S1136W, G1218R, E1219Q, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223087-plasmid-map-sequence-id-447656

    Enlarge

  21. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid
    #100597
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100597-plasmid-map-sequence-id-207349

    Enlarge

  32. TFORF1095

    Plasmid
    #143923
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    EN1 (EN1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143923-plasmid-map-sequence-id-391582

    Enlarge

  34. pRL_GI_PCB28

    Plasmid
    #98745
    Purpose
    For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    heme oxygenase 1 (TED4 Mustard Weed)
    Phycocyanobilin:ferredoxin oxidoreductase
    Use
    Synthetic Biology
    Expression
    Yeast
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    ADH1m and PGK1
    Available Since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98745-plasmid-map-sequence-id-189854

    Enlarge

  35. pYPQAT13

    Plasmid
    #223385
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223385-plasmid-map-sequence-id-453298

    Enlarge

  37. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128940-plasmid-map-sequence-id-254597

    Enlarge

  40. pcDNA3.1-Flag-RBM24

    Plasmid
    #208052
    Purpose
    Expressing N-ter Flag tagged human RBM24 (ENST00000379052) in mammalian cells
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208052-plasmid-map-sequence-id-418311

    Enlarge

  43. pYPQ166

    Plasmid
    #109328
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter
    Depositor
    Yiping Qi
    Article
    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.
    Insert
    zCas9
    Use
    CRISPR; Gateway compatible zcas9 entry clone
    Tags
    3x FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Available Since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    109328-plasmid-map-sequence-id-210215

    Enlarge

Showing: 1001 - 1050 of 166787 results