162,660 results

1. pDONR223_XPO1_WT

   Plasmid

   #81812

   Purpose

   Gateway Donor vector containing XPO1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   XPO1 (XPO1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MSCVhygro-F-G9a

   Plasmid

   #41721

   Purpose

   Retroviral expression of human G9a in mammalian cells

   Depositor

   Kai Ge

   Article

   Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

   Insert

   G9a (EHMT2 Human)
   

   Use

   Retroviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CMV-GFP-NMHC II-A

   Plasmid

   #11347

   Depositor

   Robert Adelstein

   Article

   Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

   Insert

   NMHC II-A (MYH9 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDHt-SK

   Plasmid

   #63615

   Purpose

   The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

   Depositor

   Seogchan Kang

   Article

   Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

   Type

   Empty backbone

   Use

   Fungal expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pC1-SypHer3s

   Plasmid

   #108118

   Purpose

   Ratiometric pH probe with enhanced brightness for mammalian cells expression.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s
   

   Use

   Tags

   ratiometric YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pINDUCER21-RORA

   Plasmid

   #51303

   Purpose

   Expression of RORA after doxycycline administration

   Depositor

   George Daley

   Article

   Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

   Insert

   RAR-related orphan receptor A (RORA Human)
   

   Use

   Lentiviral

   Tags

   n/a

   Expression

   Mutation

   full length ORF

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MS2-HB

   Plasmid

   #35573

   Depositor

   Marian Waterman

   Article

   Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

   Insert

   MS2 Coat Protein (cp Bacteriophage)
   

   Use

   Retroviral

   Tags

   HTBH

   Expression

   Mammalian

   Mutation

   Insert is a tail-to-head tandem placement of two …

   Promoter

   CMV

   Available since

   May 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEVOL-pylT-AznLRS

   Plasmid

   #172482

   Purpose

   For E.Coli expression, 306L309L348I384F411W

   Depositor

   Wenshe Liu

   Article

   Wang et al Angew Chem Int Ed Engl. 2017 Feb 1;56(6)

   Insert

   AznLRS and pylTcua
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK647

   Plasmid

   #71701

   Purpose

   Produces E. coli thioredoxin reductase (EcTrxB)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin reductase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF1037

    Plasmid

    #144269

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    WT1 (WT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSin-NE1m

    Plasmid

    #172883

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

    Depositor

    J. Julius Zhu

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Insert

    NE SENSOR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    sp6

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PB CAG-mTagBFP2-T2A-iCreERT2

    Plasmid

    #177308

    Purpose

    CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pB80-hKif5b(1-560)rigor-2xmNeongreen

    Plasmid

    #174649

    Purpose

    Live-cell marker for stable microtubules

    Depositor

    Lukas Kapitein

    Article

    Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

    Insert

    hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
    

    Use

    Tags

    mNeongreen-mNeongreen

    Expression

    Mammalian

    Mutation

    rigor mutation G234A

    Promoter

    Chicken beta-actin

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 18 pCOLI_A_Trx

    Plasmid

    #160255

    Purpose

    N-term His-Thioredoxin 3C cleavable Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    6xHis and Thioredoxin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA4-AMBRA1-3xFLAG

    Plasmid

    #174157

    Purpose

    Vector for expression of 3xFLAG-tagged wild-type AMBRA1

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBAD-Lime

    Plasmid

    #191255

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein Lime

    Depositor

    Robert Campbell

    Article

    Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

    Insert

    Lime
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

    Plasmid

    #217340

    Purpose

    crRNA array targeting CD81, B2M, KIT, CD55

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Klotho (secreted)

    Plasmid

    #17713

    Depositor

    Hal Dietz

    Article

    Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

    Insert

    Klotho (KL Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDH-CAG-IntCCCre-T2A-CopGFP

    Plasmid

    #160501

    Purpose

    Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    CopGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A)

    Plasmid

    #188384

    Purpose

    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mCherry-Sec61β

    Plasmid

    #172445

    Purpose

    Expression of ER protein Sec61beta with an N-terminal mCherry tag

    Depositor

    Stephen Royle

    Article

    Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Protein transport protein Sec61 subunit beta
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-TRE-mTurquoise2-WPRE

    Plasmid

    #104110

    Purpose

    AAV-mediated expression of mTurquoise2 under the TRE promoter.

    Depositor

    Takeshi Imai

    Article

    Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

    Insert

    mTurquoise2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKO403_lacZ_Sp

    Plasmid

    #174726

    Purpose

    Bifidobacterium-E.coli shuttle vector for seamless gene cloning

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-LacR-TopBP1

    Plasmid

    #31317

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

    Insert

    TopBP1 (TOPBP1 Human)
    

    Use

    Tags

    FLAG and LacR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SIRT7 Flag

    Plasmid

    #13818

    Purpose

    Mammalian expression of human SIRT7 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT7 (SIRT7 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Missing exon 2

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLentiCas9-BFP

    Plasmid

    #78545

    Purpose

    Fusion protein Cas9+BFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Fusion protein with Cas9

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUFlip-floxed2A-mRFP-; gcry1:BFP -0

    Plasmid

    #173886

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; gcry1: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV5B-TGFbeta receptor II wt

    Plasmid

    #11766

    Depositor

    Jeff Wrana, Joan Massague

    Article

    Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

    Insert

    TGFbeta receptor II (TGFBR2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pLenti PGK V5-LUC Puro (w543-1)

    Plasmid

    #19360

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ICMAB - pET22b T7 EncFtn Mms6

    Plasmid

    #225538

    Purpose

    Construction of intracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Ferroxidase
    Material binding peptite
    Iron transporter protein
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. WN10150

    Plasmid

    #80443

    Purpose

    Expresses dead (908A) AsCpf1

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    nuclease dead (908A) AsCpf1
    

    Use

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    nuclease dead (D908A)

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAV pmSyn1-EBFP-Cre

    Plasmid

    #51507

    Purpose

    Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV5

    Insert

    EBFP-Cre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pmSyn1

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. STAT3(Y705F)-TAL-Luc

    Plasmid

    #46933

    Purpose

    Luciferase Reporter for mutant STAT3

    Depositor

    Afshin Dowlati

    Article

    Dabir et al Mol Cancer Res. 2009 Nov;7(11):1854-60.

    Insert

    STAT3(Y705F) (STAT3 Human)
    

    Use

    Luciferase

    Tags

    FLAG

    Expression

    Mutation

    Tyrosine 705 to Phenylalanine

    Promoter

    TA Luciferase

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pcDNA3.1(+)-HA-Flag-natT1R2

    Plasmid

    #113944

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-839

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. UAS:bloSwitch

    Plasmid

    #108355

    Purpose

    UAS driven green to red switch reporter for B3 recombinase expression

    Depositor

    Harold Burgess

    Article

    Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

    Insert

    UAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Zebrafish codon optimized

    Promoter

    lac

    Available since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. lentiCRISPR v2-sgUpf1

    Plasmid

    #161810

    Purpose

    guide RNA for Upf1

    Depositor

    Yi Zhang

    Article

    Fu et al Sci Adv. 2020 May 29;6(22):eaay5181. doi

    Insert

    sgRNA targeting mouse Upf1 (Upf1 Synthetic, Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAC-14-LEXA-QF

    Plasmid

    #46104

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    LexA-QF
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA3_cAMPFIRE-L

    Plasmid

    #182279

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGWB424

    Plasmid

    #74818

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LentiCRISPRv2-Opti

    Plasmid

    #163126

    Purpose

    LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3-DN-hCUL4A-FLAG

    Plasmid

    #15821

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin4A (CUL4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N440

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBE14

    Plasmid

    #174653

    Purpose

    In vivo Cre-lox circularization in E. coli

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    Cre
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGEX4T1-human beta-catenin full length

    Plasmid

    #132609

    Purpose

    expresses human beta-catenin full length in E. coli

    Depositor

    Wenqing Xu

    Article

    Xing et al Structure. 2008 Mar;16(3):478-87. doi: 1

    Insert

    beta-catenin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. LV-Sox10MCS5-GFP

    Plasmid

    #115783

    Purpose

    This plasmid encodes for Sox10-MCS5-GFP reporter. Cell carrying this construct expresses green fluorescence under the control of SOX-MCS5 enhancer conjugated with cfos basal promoter.

    Depositor

    Fraser Sim

    Article

    Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

    Insert

    mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
    

    Use

    Lentiviral

    Tags

    cfos basal promoter conjugated MCS5 promoter fuse…

    Expression

    Mutation

    Promoter

    SOX10 Multiple Species Conserved enhancer element…

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCMV3flag8SHARPIN

    Plasmid

    #50014

    Purpose

    Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

    Depositor

    Martin Dorf

    Article

    Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

    Insert

    SHANK-associated RH domain interactor (SHARPIN Human)
    

    Use

    Tags

    3 x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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46. eGFP-proα1(I)

    Plasmid

    #119843

    Purpose

    Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α1 chain (Col1a1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Col1a1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAGM47523

    Plasmid

    #153221

    Purpose

    Level 0 zCas9i vector

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    SpCas9 with introns and 2 NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase

    Plasmid

    #231128

    Purpose

    Expresses xylanase via a cSAT2.0 scheme

    Depositor

    Xiaofeng Yang

    Article

    Huang et al Trends Biotechnol. 2024 Nov 12:S0167-779

    Insert

    xylanase
    

    Use

    Tags

    cSAT2.0

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCMV-G-Rac1

    Plasmid

    #180575

    Purpose

    ddGFP sensor of Rac1

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddGFP sensor of Rac1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCMV-SpRYc-ABE8e-EGFP

    Plasmid

    #208340

    Purpose

    Expresses SpRYc-ABE8e in mammalian cells

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    ecTadA(8e)-nSpRYc
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CMV

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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