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167,150 results

Showing: 1051 - 1100 of 167150 results

  1. pHAGE-CMV-Flag-AAK1_IDG-K

    Plasmid
    #135280
    Purpose
    Gateway destination clone of AAK1 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Ben Major
    Insert
    Flag-AAK1 (AAK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135280-plasmid-map-sequence-id-311570

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  12. pGMCK-T38S38-750-luxAB-DAS+4

    Plasmid
    #49518
    Purpose
    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    T38S8-P750-luxAB-DAS+4
    Tags
    DAS+4 (AANDENYSENYADAS)
    Expression
    Bacterial
    Promoter
    P750
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49518-plasmid-map-sequence-id-73896

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  13. pBOB-VE-cadherin-tdTomato

    Plasmid
    #118733
    Purpose
    To express VE-cadherin tagged with tdTomato on C-terminus
    Depositor
    John Cooper
    Insert
    Cadherin 5 (CD144) (CDH5 Human)
    Use
    Lentiviral
    Tags
    tdTomato
    Mutation
    I503T (Common SNP-see comments)
    Promoter
    CMV
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    118733-plasmid-map-sequence-id-229276

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  14. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

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  27. pLVX-Tet3G blasticidin

    Plasmid
    #128061
    Purpose
    Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128061-plasmid-map-sequence-id-255115

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  29. pFA6a-link-ymTurquoise2-Kan

    Plasmid
    #224092
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with Kan selection
    Depositor
    Bas Teusink
    Insert
    ymTurquoise2
    Tags
    ymTurquoise2
    Expression
    Yeast
    Available Since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224092-plasmid-map-sequence-id-453797

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  33. pAG/MNase

    Plasmid
    #123461
    Purpose
    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.
    Depositor
    Steven Henikoff
    Article
    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1
    Insert
    Protein A-Protein G-Micrococcal Nuclease
    Tags
    6xHIS and HA
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    123461-plasmid-map-sequence-id-417805

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  39. pRGEB32

    Plasmid
    #63142
    Purpose
    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.
    Depositor
    Yinong Yang
    Article
    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Rice snoRNA U3 promoter for gRNA/PTG expression a…
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63142-plasmid-map-sequence-id-339931

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  40. pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX

    Plasmid
    #154910
    Purpose
    1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.
    Depositor
    Clare Buckley, Jonathan Clarke
    Article
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Insert
    Phytochrome B (PHYB Mustard Weed, Synthetic)
    Use
    Xenopus/avian/zebrafish
    Tags
    CAAX membrane moiety and mCherry
    Expression
    Mammalian
    Mutation
    1-621 amino acids of Arabidopsis PHYB
    Promoter
    CMV
    Available Since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154910-plasmid-map-sequence-id-318440

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  45. pShuttle-CMV-GFP U6promoter

    Plasmid
    #128049
    Purpose
    Adenoviral shuttle vector for shRNA expression from U6 promoter with GFP marker
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Adenoviral
    Expression
    Mammalian
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128049-plasmid-map-sequence-id-255046

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Showing: 1051 - 1100 of 167150 results