161,186 results
-
Plasmid#142090PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertIRF7 (IRF7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-PhoCl1-6His
Plasmid#164033PurposeExpressed PhoCl1 in a pET expression vectorDepositorInsertPhoCl1
UseTags6xHis TagExpressionBacterialMutationPromoterT7 promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5
Plasmid#111818PurposeQuadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2ADepositorUseCloning intermediateTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCgsgRNA_crtYf
Plasmid#153338PurposeDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; SpcrDepositorInsertcrRNA targeting crtYf
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD-CRY2
Plasmid#28243PurposeExpresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activationDepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsGal4BDExpressionYeastMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-LILRA3-Fc(DAPA)-AviTag-6xHis
Plasmid#156846PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertLILRA3 (LILRA3 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-MT2A
Plasmid#11613DepositorInsertmetallothionein 2A (MT2A Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCS4092
Plasmid#131741PurposeTheophylline switch Theo-CAUAADepositorInsertTheophylline switch Theo-CAUAA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ETV6_NTRK3
Plasmid#205817PurposeExpress mEGFP-tagged fusion protein, ETV6_NTRK3 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPML1 (GB0045)
Plasmid#68164PurposeProvides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid partDepositorInsertAtML1 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Opa1
Plasmid#191963PurposeExpression of OPA1.DepositorInsertOpa1 (Opa1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-Sec31A
Plasmid#226582PurposeEncodes Sec31A protein labeled with mNeonGreenDepositorInsertSec31A (Protein transport protein) (SEC31A Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA Display H3_A/Hong Kong/1/1968
Plasmid#206506PurposeExpresses H3_Hong Kong/1/1968 in the surface of mammalian cellsDepositorInsertH3_A/Hong Kong/1/1968
UseTags3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…ExpressionMammalianMutationdeleted amino acids 1651 to 1735PromoterCMVAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
CBE-T1.14
Plasmid#193277PurposeExpression of CBE-T1.14 base editor in mammalian cells, CMV promoterDepositorInsertCBE-T1.14
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYU-2-Env
Plasmid#133997PurposeEnv gene from HIV-1 YU-2 in pcDNA3.1 expression vectorDepositorInsertEnvelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTnos (GB0037)
Plasmid#68188PurposeProvides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid partDepositorInsertNos terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-Intb1
Plasmid#205193PurposeThis vector encodes of a synCAM (Intb1) and GFPDepositorInsertsynCAM Intb1 and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 cat cassette
Plasmid#117119PurposeChloramphenicol resistance cassetteDepositorInsertChloramphenicol resistance cassette for Bacillus subtilis selection
UseGolden gate donor vector for gene targeting in ba…TagsExpressionMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-Rncp-iGluSnFR1
Plasmid#107336PurposeRed fluorescent glutamate sensor with ncp topology. Membrane-displayedDepositorInsertRncp-iGluSnFR1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Calnexin-HT8-mGold
Plasmid#183989PurposeExpresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.DepositorInsertCalnexin (CANX Human)
UseTagsHaloTag8 (C61S, C262S), mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGrin2b
Plasmid#124851PurposeMutagenesis of Grin2bDepositorInsertGrin2b (Grin2b Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.V1
Plasmid#127847Purposenon-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.V1 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE4-SsAPOBEC3B
Plasmid#138343PurposeMammalian expression plasmid for BE4-SsAPOBEC3BDepositorInsertBE4-SsAPOBEC3B
UseSynthetic BiologyTagsBP-NLSExpressionMutationPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR.CMV
Plasmid#32688PurposeMammalian expression and gateway entry vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB8
Plasmid#63109PurposeExpression of HSPB8 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb8 (HSPB8 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag16-Ecad
Plasmid#205197PurposeThis vector encodes of the synCAM (Ecad) and GFP nanobody LAG16DepositorInsertsynCAM Ecad and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-APX(W41F)-NES
Plasmid#42608DepositorArticleInsertsoybean APX W41F (dimeric) -NES
UseTagsFlagExpressionMammalianMutationW41F mutationPromoterCMVAvailable sinceMarch 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VPS15-TSF
Plasmid#203563PurposeMammalian expression of VPS15 with TwinStrep-Flag tagDepositorInsertVPS15 (PIK3R4 Human)
UseTagsTwinStrep FlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-HsSmg6_H
Plasmid#146475PurposeMammalian Expression of HsSmg6DepositorInsertHsSmg6 (SMG6 Human)
UseTagsExpressionMammalianMutationtwo silent mutations T594C, A699G and two non sil…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag ILK
Plasmid#20505DepositorInsertILK (ILK Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-JAM-B
Plasmid#205191PurposeThis vector encodes of a synCAM (JAM-B) and GFPDepositorInsertsynCAM JAM-B and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLC-HA-Nedd8-P2A-Hygro
Plasmid#124306PurposeLentiviral vector for expression of HA tagged Nedd8-P2A-Hygro casette from a CMV promoterDepositorInsertNedd8 (NEDD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPA2-B1_Z
Plasmid#148113PurposeMammalian Expression of HshnRNPA2-B1DepositorInsertHshnRNPA2-B1 (HNRNPA2B1 Human)
UseTagsExpressionMammalianMutationsplicing variant of hnRNPA2 variant B1. Exon 6 an…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGilesSHdI_psmyc_His6_triple red
Plasmid#170047PurposeMono-Responsive Reporter Gene Constructs for triple redDepositorInsertsmScarlet
COmScarlet
mRuby3
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmiRFP713-T2A-HO1
Plasmid#197246PurposeExpresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegansDepositorInsertwmiRFP713-T2A-HO1
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
(114) pcDNA3.1-GFP-Flag-JunB-EPEA
Plasmid#160743PurposeExpresses a GFP tagged JunB with Flag and EPEA tags.DepositorInsertGFP-Flag-JunB-EPEA (JUN Human)
UseTagsEPEA, Flag, and GFPExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
p11d-sctRPA
Plasmid#102614PurposeExpression of S. cerevisiea RPA in E.coliDepositorUseTagsExpressionBacterialMutationRFA2 has intron deletedPromoterT7Available sinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV.MaCPNS1
Plasmid#185136Purposenon-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-MaCPNS1 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-MUC4
Plasmid#205189PurposeThis vector encodes of a synCAM (MUC4) and GFPDepositorInsertsynCAM MUC4 and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
VRK1_HUMAN_D0
Plasmid#79684PurposeThis plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVRK1 (VRK1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
qMaLioffG
Plasmid#212825Purposegreen fluorescent FLIM indicator for endogenous ATP imagingDepositorInsertqMaLioffG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT PUR mGSK3AWT
Plasmid#35159DepositorInsertGSK-3α (Gsk3a Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMK312 (TOP2A CRISPR)
Plasmid#140654PurposeTOP2A tagging CRISPRDepositorInsertTOP2A (TOP2A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti.CMV/TO_PRKAA2
Plasmid#74447PurposeLentiviral expression vector encoding untagged WT human AMPK alpha 2DepositorInsertAMPK alpha 2 (PRKAA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX nsp1 CoV2
Plasmid#175512PurposeFor protein expression of WT nsp1 CoV2DepositorInsertnsp1 CoV2
UseTagsGST tagExpressionBacterialMutationPromotertac promoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI1_G-alpha
Plasmid#110012PurposeProtein expression and purification of GNAI1_G-alphaDepositorInsertGNAI1_G-alpha (GNAI1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
EZ-L560
Plasmid#173028PurposeEncodes OptoAMP2 circuitDepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationPromoterAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDule-3-nitroTyrosine (5B)
Plasmid#85498PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-NLS-dCas9-NLS
Plasmid#99908PurposeFor the expression of NLS-dCas9-NLSDepositorInsertNLS-dCas9-NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only