175,647 results
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Plasmid#178329PurposeFluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857DepositorHas ServiceAAV1InsertpAAV hSyn iGluSnFR3 v857.PDGFR
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and PDGFR TM DomainExpressionMammalianAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
RpHLuorin2
Plasmid#180227Purposefor bacterial expression of recombinant ratiometric pHluorin2DepositorInsertRatiometric pHLuorin2
TagsHis-tag followed by thrombin cleavage siteExpressionBacterialPromoterT7Available SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHN781
Plasmid#178828PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
TagsHis8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available SinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_SF3B3-Xon_Luciferase
Plasmid#174659PurposePlasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.DepositorInsertXon cassette
UseLuciferaseExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAB35
Plasmid#175787PurposeExpresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tagDepositorInsertSidK (1-278)
Tags3xFLAG and 6xHis TEVExpressionBacterialAvailable SinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mBeRFP
Plasmid#175173Purposelarge-stokes shift red fluorescent protein mBeRFPDepositorInsertmBeRFP
Available SinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5B
Plasmid#172203PurposeExpresses KIF5B tagged with eGFP in mammalian cellsDepositorAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
bact2-mKate2-P2A-TurboID-dVHH[GFP]
Plasmid#163857PurposeZebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBHDepositorInsertTurboID-VHH[EGFP]
UseZebrafish expressionTagsmKate2-P2A-TurboIDAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
Plasmid#129631PurposeVisualization of Akt and ERK activityDepositorInsertH2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU294
Plasmid#167689PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3_MERS_D12
Plasmid#170448PurposeExpress MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.DepositorInsertMERS spike D12 (S MERS)
ExpressionMammalianMutationlast 12aa deletion in c-terminal tailPromoterCMVAvailable SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONRp5-p2 SspB-mCherry-CShroom3
Plasmid#170977PurposeC-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.DepositorInsertCShroom3 (Shroom3 Mouse)
UseGateway CloningTagsSspB and mCherryMutationIsoform X2, deleted aminoacids 1 - 1388Available SinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sst44-GFP
Plasmid#172310PurposeSST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneuronsDepositorInsertSst44
UseAAVPromoterpB-GlobinAvailable SinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE30-HyPer7
Plasmid#141071PurposeBacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
TagsHisx6ExpressionBacterialPromoterT5Available SinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-NlucP
Plasmid#162595PurposeC-terminally PEST-tagged NanoLuc under TRE3G promoterDepositorInsertNanoLuc-PEST
TagsPESTExpressionMammalianPromoterTRE3G promoterAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE
Plasmid#161926PurposeLentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAGDepositorInsertrTetR(SE-G72P)
UseLentiviral and Synthetic BiologyTags3XFLAGExpressionMammalianPromoterpEFAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL21-mCherry
Plasmid#164410PurposeRetroviral expression plasmid of sgRNA with mCherryDepositorTypeEmpty backboneUseCRISPR and RetroviralExpressionMammalianPromoterU6 promoter for crRNA expression and EFS promoter…Available SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.(Cyto)iGlucoSnFR
Plasmid#164513PurposeGreen glucose sensor, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2 (Plasmid #244111) instead of this plasmid.DepositorInsert(Cyto)iGlucoSnFR
UseAAVMutationSynthetic ConstructPromoterGFAPAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mCherry-Nanobody (LaM-2)
Plasmid#162276PurposeExpresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.DepositorInsertmCherry-Nanobody (LaM-2)
TagsGSTExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
JD639
Plasmid#160398PurposeBinary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformationDepositorInsert35S::vitis miR396-resistant GRF4-GIF1 chimera
UseBinary vectorAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mGreenLantern
Plasmid#161913PurposemGreenLantern fluorescent protein: bacterial expression plasmidDepositorInsertmGreenLantern
Tags6xHisExpressionBacterialPromoteraraBAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK395 (AAVS1 CMV-OsTIR1(F74A))
Plasmid#159992PurposeExpression of OsTIR1(F74A) in human cellsDepositorInsertCMV-OsTIR1(F74A)
ExpressionMammalianAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)
Plasmid#154824Purpose(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegansDepositorInsertPeft-3::wrmScarlet::tbb-2 UTR
ExpressionWormPromotereef-1A.1 (eft-3)Available SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETduet+p300HAT
Plasmid#157793PurposeExpression of p300 HAT domainDepositorAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-mCherry-G12V-KRAS-IRES-Blast
Plasmid#153336PurposeLentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cellsDepositorAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE_hygro_mRFP1_NRF2
Plasmid#136579Purposemammalian expression of mRFP1-tagged human NRF2DepositorAvailable SinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR A757
Plasmid#140829PurposeAIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757DepositorInsertAIMTOR A757 (ULK1 Human)
TagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…Available SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
P307-FBP_6
Plasmid#139697PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathwayDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH2B-emiRFP703
Plasmid#136567PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertH2B-emiRFP703
TagsemiRFP703ExpressionMammalianPromoterCMVAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
M3-M14-dCas9
Plasmid#134779PurposeExpression of fusion protein M3-M14-dCas9 in Mammalian cellsDepositorInsertExpressionMammalianPromoterCMVAvailable SinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Gria1-GFP KI
Plasmid#131489PurposeEndogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)DepositorAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 acGFP
Plasmid#128047PurposeMammalian expression vector with N terminal AcGFPDepositorTypeEmpty backboneTagsAcGFPExpressionMammalianAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pinducer 20 DN-KASHΔPPPL
Plasmid#129280PurposeTet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPLDepositorInsertSYNE1 (SYNE1 Human)
UseLentiviralTagsmcherryMutationthe last 4 amino acids (PPPL) of the KASH domain …PromoterCMVAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
scOrange chromoprotein
Plasmid#117840PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coliDepositorInsertpromoter, RBS, scOrange
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDL278_P23-DsRed-Express2
Plasmid#121505PurposeE. coli-streptococcal shuttle vector that expresses rfpDepositorInsertDsRed-Express2
UseE. coli streptococcal shuttle vectorPromoterP23Available SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRX-INU-FLAG-FAM134B
Plasmid#128260PurposeExpresses FAM134B in mammalian cellsDepositorAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDonor_AcCAST_kanR
Plasmid#127925PurposeKanamycin resistance donor for AcCASTDepositorInsertKanR
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLCKO2
Plasmid#125518PurposeLentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH-MBP-CbAgo
Plasmid#127707PurposepET His6 MBP TEV CbAgoDepositorInsertCbAgo
TagsMPBExpressionBacterialPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRT
Plasmid#120274PurposeTA-cloning vector of PCR-productsDepositorTypeEmpty backboneAvailable SinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB
Plasmid#122668PurposeC terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.DepositorInsertHNRNPA1 (HNRNPA1 Human)
UseLentiviralTagsmCherry-SspBExpressionMammalianMutationContains only amino acids 186-230PromoterSFFVAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mNeonGreen
Plasmid#99131PurposeAn AAV genome with tet-inducible expression of the fluorescent protein mNeonGreenDepositorInsertmNeonGreen
UseAAVExpressionMammalianPromoterTREAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-gal4-VP16-B-actin
Plasmid#117817PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ROR1
Plasmid#116789PurposeLentiviral expression of ROR1DepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ALK
Plasmid#116712PurposeLentiviral expression of ALKDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK348 (BSD-P2A-mAID-mClover)
Plasmid#121182PurposeN-terminal taggingDepositorInsertBSD-P2A-mAID-mClover
UseCRISPRAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc
Plasmid#117728PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc geneDepositorInsertNanoLuc
UseLuciferaseExpressionBacterialAvailable SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
2Flag-pDEST-N
Plasmid#118371PurposeGateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2FlagExpressionMammalianAvailable SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)
Plasmid#112022PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-betaDepositorInsertNYESO alpha/beta into TRBC1 HDRDT
UseCRISPR and Synthetic BiologyAvailable SinceNov. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-152
Plasmid#118566PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn7 delivery vectorExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only