162,602 results

1. EMTB-mNeonGreen

   Plasmid

   #137802

   Purpose

   Visualization of microtubules

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   Ensconsin microtubule binding domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSEM270 - MCS mosTI hygroR target

   Plasmid

   #159826

   Purpose

   Single copy or array insertion by MosTI using split hygroR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   MCS mosTI hygroR target
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEGFP hsp70 K71E

   Plasmid

   #15216

   Depositor

   Lois Greene

   Article

   Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

   Insert

   hsp70 K71E (HSPA1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K71E, ATPase-deficient mutant.

   Promoter

   Available since

   June 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmVen-DHX9

   Plasmid

   #211409

   Purpose

   mammalian expression of human DHX9 N-terminally fused to mVenus

   Depositor

   Helmut Pospiech

   Insert

   DHX9 (DHX9 Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 2MLS-HyperNova/pcDNA3

   Plasmid

   #221457

   Purpose

   Expressing HyperNova in mitochondria

   Depositor

   Kiwamu Takemoto

   Article

   Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

   Insert

   HyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. (401) pAAV CMV-ZsGreen U6-gRNA

   Plasmid

   #163021

   Purpose

   Fluorescent reporter for Sa gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Zs Green
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBT280

   Plasmid

   #122610

   Purpose

   express evoFERNY-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoFERNY-BE4max
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H102P D104N

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. alpha Synuclein-VC

   Plasmid

   #89471

   Purpose

   expresses human alpha Synuclein fused to the C-terminal part of Venus

   Depositor

   Bradley Hyman, Tiago Outeiro

   Article

   Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   C-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pH6HTC_ENO1-Halo

   Plasmid

   #175330

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   ENO1 (ENO1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-OXA1L

    Plasmid

    #116768

    Purpose

    Lentiviral expression of OXA1L

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    OXA1L (OXA1L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pST39

    Plasmid

    #64009

    Purpose

    original polycistronic cloning vector for plasmid suite (generation I)

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Opie2-PuroR

    Plasmid

    #131613

    Purpose

    Expresses PuroR and dsRed.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Inserts

    PuroR
    dsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Opie2 and hr5Ie1

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCIneo RYR-1

    Plasmid

    #75113

    Purpose

    Clone encoding rabbit ryanodine receptor in mammalian expression vector

    Depositor

    Paul Allen, Robert Dirksen

    Article

    Nakai et al J Biol Chem. 1998 May 29;273(22):13403-6

    Insert

    Ryanodine receptor 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-WT-VWF

    Plasmid

    #124794

    Purpose

    Expresses multimeric human VWF with C-terminal c-Myc tag

    Depositor

    Sriram Neelamegham

    Article

    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

    Insert

    VWF (VWF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-Flag-H3.3-polyA

    Plasmid

    #59780

    Purpose

    Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    H3F3B (H3-3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCGN-ATF6 (1-373)

    Plasmid

    #27173

    Purpose

    For mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.

    Depositor

    Ron Prywes

    Article

    Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains only aa 1-373 (please see depositor comm…

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMSCV-IRES-mAmetrine 1.1 FP

    Plasmid

    #52113

    Purpose

    a bicistronic IRES-mAtetrine containing retroviral vector

    Depositor

    Dario Vignali

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-mAmetrine 1.1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSLIK-Hygro

    Plasmid

    #25737

    Purpose

    3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDuet TDP43 WT

    Plasmid

    #27462

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Coexpression

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCh-KIF5B*-strep

    Plasmid

    #120164

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5B (Kif5b Mouse)
    

    Use

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF5B: 1-555 aa

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TRE-ChrimsonR-mCherry

    Plasmid

    #92207

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    ChimsonR-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-smURFP-T2A-mCardinal

    Plasmid

    #80348

    Purpose

    Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCardinal.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    smURFP
    mCardinal
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEG BacMam N term StrepII eGFP 3C

    Plasmid

    #160683

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    StrepII eGFP 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-HA-MyD88-FW/AA

    Plasmid

    #12289

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    MyD88 (MYD88 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F285A/W286A

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

25. NPC1 His6 EGFP

    Plasmid

    #53521

    Depositor

    Matthew Scott

    Article

    Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

    Insert

    NPC1
    

    Use

    Tags

    EGFP and His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1/ZMYM2-FGFR1

    Plasmid

    #167486

    Purpose

    Mammalian expression vector for the ZMYM2-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

    Depositor

    Nicholas Cross

    Article

    Smedley et al Neoplasia. 1999 Oct;1(4):349-55. doi: 10

    Insert

    ZMYM2-FGFR1 (FGFR1, ZMYM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115219

    Purpose

    Expresses PspCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PspCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 21, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. TRE-SEAP

    Plasmid

    #210511

    Purpose

    SEAP reporter vector for LAUNCHER system

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    secreted alkaline phosphatase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pC1-CMV-DAAO-NES

    Plasmid

    #141107

    Purpose

    Expresses DAAO with nuclear export signal in mammalian cells

    Depositor

    Vsevolod Belousov

    Article

    Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

    Insert

    DAAO-NES
    

    Use

    Tags

    Nuclear export signal

    Expression

    Mammalian

    Mutation

    deleted DAAO amino acids 367-368

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MOS

    Plasmid

    #64120

    Purpose

    A modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genes

    Depositor

    Linzhao Cheng

    Article

    Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

    Insert

    OCT4-2A-SOX2 (POU5F1, SOX2 Human)
    

    Use

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

    Plasmid

    #35152

    Depositor

    Taek Soon Lee, Jay Keasling

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    R364S in Mevalonate Kinase

    Promoter

    LacUV5, pTrc

    Available since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Flag-hPIASx beta

    Plasmid

    #15210

    Depositor

    Ke Shuai

    Article

    Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

    Insert

    PIASx beta (PIAS2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCas9cur

    Plasmid

    #71538

    Purpose

    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    Plasmid curing system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. hNCAM2

    Plasmid

    #84473

    Purpose

    expression of hNCAM2 in mammalian cells

    Depositor

    Vladimir Sytnyk

    Article

    Leshchyns'ka et al Nat Commun. 2015 Nov 27;6:8836. doi: 10.

    Insert

    NCAM2 (NCAM2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGT-Kh2

    Plasmid

    #122587

    Purpose

    MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    beta-lactamase/sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRSET FLIPPi-200u

    Plasmid

    #13555

    Depositor

    Wolf Frommer

    Article

    Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

    Insert

    FLIPPi G162A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    G162A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCI-neo-cOVA

    Plasmid

    #25097

    Depositor

    Maria Castro

    Article

    Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

    Insert

    cytoplasmic ovalbumin
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCIRCgp41-1

    Plasmid

    #74227

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    gp41-1 C-intein and gp41-1 N-intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Ddo_sfrp-1

    Plasmid

    #107637

    Purpose

    In situ hybridization and RNAi studies in planarians

    Depositor

    Alejandro Sánchez

    Article

    Accorsi et al The American Biology Teacher 79(3):208-2

    Insert

    sfrp-1
    

    Use

    RNAi; In situ hybridization

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAM5505

    Plasmid

    #132664

    Purpose

    pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Insert

    MobA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    please see depositor comments below

    Promoter

    p1,p3

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3

    Plasmid

    #170163

    Purpose

    Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2s-P2A-H2B-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pDule-pCNF

    Plasmid

    #85494

    Purpose

    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

    Insert

    para-cyanophenylalanine Mj synthetase
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pDestTol2pA2_ubi:EGFP

    Plasmid

    #27323

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    ubiquitin:EGFP reporter
    

    Use

    Tol2 transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. AbVec2.1-mIglc2

    Plasmid

    #127156

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant region

    Depositor

    Hedda Wardemann

    Article

    Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

    Insert

    Iglc2 (Iglc2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCMV-CD63-L7Ae

    Plasmid

    #180503

    Purpose

    Human CD63-L7Ae fusion protein

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    Human CD63-L7Ae fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. FUG-Pten

    Plasmid

    #140732

    Purpose

    Lentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion protein

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-Pten (PTEN Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hUbiC

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. FKBP-mCherry

    Plasmid

    #108122

    Purpose

    Recruitable fluorophore

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    FKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pHR-UCOE-EF1a-Zim3-dCas9-BFP

    Plasmid

    #188775

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9-mTagBFP2
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

    Plasmid

    #92142

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLKO human shRNA 1 DEPTOR

    Plasmid

    #21335

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR shRNA 1 (DEPTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only