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159,238 results

Showing: 1301 - 1350 of 159238 results

  3. pT077

    Plasmid
    #137879
    Purpose
    Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP
    Depositor
    Lindy Barrett
    Article
    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.
    Insert
    TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    137879-plasmid-map-sequence-id-271982

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  8. pLS-mP

    Plasmid
    #81225
    Purpose
    Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.
    Depositor
    Nadav Ahituv
    Article
    Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Derived from pGL4.23
    Available since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    81225-plasmid-map-sequence-id-315921

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  11. pR26 CAG/GFP Dest

    Plasmid
    #74281
    Purpose
    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning
    Depositor
    Ralf Kuehn
    Article
    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi
    Insert
    Rosa26 5-homology region
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    74281-plasmid-map-sequence-id-419887

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  12. LZ10 PBREBAC-H2BHalo

    Plasmid
    #91564
    Purpose
    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.
    Depositor
    James Zhe Liu
    Article
    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1
    Insert
    histone H2B-HaloTag
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91564-plasmid-map-sequence-id-180643

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  14. AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR

    Plasmid
    #191795
    Purpose
    vector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13d
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    A134V, A140V, A141V, A143V
    Promoter
    EFS, hU6
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191795-plasmid-map-sequence-id-375580

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  17. tFucci(CA)5

    Plasmid
    #153521
    Purpose
    New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.
    Insert
    tFucci(CA)5.
    Use
    Tags
    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    153521-plasmid-map-sequence-id-308996

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  18. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140002-plasmid-map-sequence-id-275516

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  24. TNFR1-YFP

    Plasmid
    #111209
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TNFRSF1A (TNFRSF1A Human)
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111209-plasmid-map-sequence-id-212987

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  28. pGDP1 NDM-1

    Plasmid
    #112883
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    NDM-1 (blaNDM-1 Klebsiella pneumoniae)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112883-plasmid-map-sequence-id-219864

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  35. pCDB179-TurboPETase

    Plasmid
    #218693
    Purpose
    Encodes codon optimized TurboPETase enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    TurboPETase
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218693-plasmid-map-sequence-id-434224

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  40. pCBC-DT2T3

    Plasmid
    #50591
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50591-plasmid-map-sequence-id-244476

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  42. pEvol-MjaYRS

    Plasmid
    #153557
    Purpose
    Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli
    Depositor
    Huiwang Ai
    Article
    Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038
    Inserts
    MJaYRS (first copy)
    MJaYRS (second copy)
    amber suppression tRNA under proK promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    glnS and pBAD
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    153557-plasmid-map-sequence-id-306290

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  49. Cas9-NAT

    Plasmid
    #64329
    Purpose
    Cas9 expression cassette carried by a yeast single-copy episome plasmid
    Depositor
    Yong-Su Jin
    Article
    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):
    Insert
    Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64329-plasmid-map-sequence-id-227458

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Showing: 1301 - 1350 of 159238 results