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173,291 results

Showing: 1301 - 1350 of 173291 results

  5. pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

    Plasmid
    #203350
    Purpose
    Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    Nano Luciferase
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Promoter
    T7
    Available Since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203350-plasmid-map-sequence-id-399148

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  6. pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64

    Plasmid
    #203349
    Purpose
    Template for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    mGreenLantern
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Promoter
    T7
    Available Since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203349-plasmid-map-sequence-id-399147

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  8. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154786
    Purpose
    Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE
    Depositor
    Robert Singer
    Article
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Fly, Synthetic)
    Use
    Lentiviral
    Tags
    GFP, V5, and mCherry
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154786-plasmid-map-sequence-id-306310

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  12. PB-TO-oNGN2

    Plasmid
    #198397
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…
    Promoter
    CAG, EF1a, and TRE3G
    Available Since
    April 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    198397-plasmid-map-sequence-id-389535

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  13. PDIA4-bio-His

    Plasmid
    #52071
    Purpose
    Expresses full-length Protein disulfide-isomerase A4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PDIA4 (PDIA4 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52071-plasmid-map-sequence-id-80296

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  17. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid
    #183409
    Purpose
    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183409-plasmid-map-sequence-id-361258

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  20. pHAGE-cDEST-APEX2

    Plasmid
    #214993
    Purpose
    Gateway compatible empty vector (lentiviral/mammalian expression) with a C-terminal APEX2 tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    APEX2-Flag
    Expression
    Mammalian
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    214993-plasmid-map-sequence-id-443330

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  31. psd44-GqQL

    Plasmid
    #46826
    Purpose
    2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    g protein alpha q (GNAQ Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Q209L (The mutation reduces GTPase activity resul…
    Promoter
    PGK promoter
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    46826-plasmid-map-sequence-id-246440

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  40. pRDA_221

    Plasmid
    #169142
    Purpose
    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.
    Depositor
    John Doench, David Root
    Insert
    EGFP , EGFP sgRNA
    Use
    CRISPR and Lentiviral
    Available Since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    169142-plasmid-map-sequence-id-334957

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  50. pLenti CMV rtTA3 Hygro (w785-1)

    Plasmid
    #26730
    Purpose
    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 
    Depositor
    Eric Campeau
    Insert
    Tetracycline repressor A3 mutant
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    26730-plasmid-map-sequence-id-334693

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Showing: 1301 - 1350 of 173291 results