9,057 results
-
-
HA-HIF1alpha-pcDNA3
Plasmid#18949DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
TagsHA-tagExpressionMammalianMutationwt cDNAAvailable SinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pk7-GR-GFP
Plasmid#15534DepositorInsertGlucocorticoid receptor (NR3C1 Human)
TagsGFPExpressionMammalianMutationThe glucocorticoid receptor was cloned into the X…Available SinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
Laconic/pcDNA3.1(-)
Plasmid#44238Purposea genetically-encoded sensor for lactate concentration, transport and metabolic fluxDepositorInsertLaconic (LACtate Optical Nano Indicator from CECs)
UseLactate biosensorExpressionMammalianPromoterCMVAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPalmitoyl-mTurquoise2
Plasmid#36209PurposeIn vivo visualization of the membrane (can be used for colocalization studies)DepositorAvailable SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Traptavidin
Plasmid#24753DepositorInsertTraptavidin
TagsHis-6ExpressionBacterialMutationStreptavidin with 2 amino acid mutations: S52G an…Available SinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
MS2_GFP
Plasmid#61764PurposeExpresses MS2 coat protein fused to GFPDepositorInsertMS2 coat protein fused to eGFP
UseLentiviralTagseGFPExpressionMammalianPromoterUbc promoterAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6b
Plasmid#207852PurposeMammalian expression of PE6b prime editorDepositorInsertPE6b
TagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationTf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…Available SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-jRGECO1a
Plasmid#61563PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRGECO1a
Tagsnuclear export signalExpressionMammalianPromoterCMVAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH NICD1
Plasmid#86500PurposeMammalian expression of Myc tagged NICD1DepositorInsertNotch1 (C-term) (Notch1 Mouse)
TagsMycExpressionMammalianMutationCytoplasmic domain only (AA 1753-2531)PromoterEF1aAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCoPURO
Plasmid#17533DepositorInsertPuromycine Acetyl Transferase
ExpressionInsectAvailable SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLX313-Firefly luciferase
Plasmid#118017PurposeConstitutive expression of Firefly luciferaseDepositorInsertFirefly luciferase
UseLentiviralExpressionMammalianPromoterEF1alphaAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a_6H-TAQ_E602D
Plasmid#166944PurposeBacterial expression of Taq DNA polymeraseDepositorInsertThermus aquaticus DNA polymerase
Tags6X HisExpressionBacterialMutationE602DPromoterT7Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Tp-gfpmut3
Plasmid#65033PurposeTpR mini-Tn7 vector, mobilizable, for GFP tagging bacteriaDepositorInsertgfpmut3
ExpressionBacterialAvailable SinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
p4xmts-mScarlet3_N1
Plasmid#189774PurposeConstruct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3DepositorInsert4xmts-mScarlet3
ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag Nprl2 pRK5
Plasmid#46333DepositorAvailable SinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKM444
Plasmid#108319PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage IntegraseDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
ExpressionBacterialAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Neo (657-2)
Plasmid#17447Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, NeoDepositorInsertGFP
UseLentiviralExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-mR7BD
Plasmid#79149Purposebacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)DepositorAvailable SinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-BFP
Plasmid#78545PurposeFusion protein Cas9+BFPDepositorInsertBFP
UseLentiviralExpressionMammalianPromoterFusion protein with Cas9Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP720-N1
Plasmid#136560PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720DepositorInsertmiRFP720
ExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W
Plasmid#72667PurposeLentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)DepositorInsertmU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-tdTomato-CNA35
Plasmid#61606Purposebacterial expression of N-terminal fusion protein of CNA35 with tdTomatoDepositorInserttdTomato and CNA35
TagsHis tagExpressionBacterialPromoterT7Available SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRKH2
Plasmid#71312PurposeTheta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.DepositorTypeEmpty backboneUseE. coli-gram positive bacteria shuttle vectorExpressionBacterialAvailable SinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-eGFP-53BP1
Plasmid#60813Purposemammalian expression vectorDepositorAvailable SinceDec. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
3XFlag DVL2 (WT)
Plasmid#24802DepositorAvailable SinceMay 14, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXPR_050
Plasmid#96925Purposelentiviral expression of gRNA scaffoldDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RB
Plasmid#60522PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX303
Plasmid#25897Purpose3rd generation lentiviral Gateway destination vector. Blasticidin selection.DepositorTypeEmpty backboneUseLentiviral; Gateway destination vectorExpressionMammalianAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
RelA cFlag pcDNA3
Plasmid#20012DepositorAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-Puro
Plasmid#86708PurposeMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K33
Plasmid#17607PurposeMammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
TagsHAExpressionMammalianMutationK33 only, other lysines mutated to arginines; P19…Available SinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
MCS-13X Linker-BioID2-HA
Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianPromoterCMVAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC1-SypHer3s-dmito
Plasmid#108119PurposeMitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.DepositorInsertSypHer3s-dmito
Tagsratiometric YFPExpressionMammalianPromoterCMVAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-hM3D(Gq)
Plasmid#45547PurposeExpresses hM3D (Gq) neuronal activatorDepositorInserthM3D (Gq)
TagsHAExpressionBacterial and MammalianPromoterCMVAvailable SinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
Plasmid#118156PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
mTLR4 flag
Plasmid#13087DepositorInsertTLR4 (Tlr4 Mouse)
TagsFlagExpressionMammalianMutationAmino acids 1-19 have been replaced with mammalia…Available SinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLX307 Luciferase
Plasmid#117734PurposeOpen reading frame vector encoding LuciferaseDepositorInsertfirefly luciferase
UseLentiviralTagsV5ExpressionMammalianPromoterEF1aAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
beta-arrestin2 mYFP
Plasmid#36917DepositorAvailable SinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)
Plasmid#179115PurposeCargo EGFP with AttP Bxb1 site (Parent minicircle)DepositorInsertCargo EGFP with AttP Bxb1 site
UseCRISPRAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-CRBN-midi
Plasmid#215330PurposeCRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader designDepositorInsertCRBN (CRBN Human)
Tags6xHis and TEVExpressionBacterialMutationcontains amino acids 41-187 followed by a Gly-Ser…PromoterlacIAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro /BRAF-V600E
Plasmid#131724PurposeMammalian Expression of Flag tagged BRAFDepositorInsertBRAF (BRAF Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationV600EPromoterCMVAvailable SinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSimple-miR302/367
Plasmid#98748PurposeEpisomal reprogrammingDepositorAvailable SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-Hylight
Plasmid#193447PurposeA fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)DepositorInsertHylight Biosensor
ExpressionMammalianPromoterCMVAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Vps34-Flag
Plasmid#24398DepositorAvailable SinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA4mt
Plasmid#58220PurposeGreen fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorInsertCEPIA4mt
TagsmycExpressionMammalianMutationGCaMP2 N263Y E282V E334D M339L F395W D436EPromoterCMVAvailable SinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Luc2-IRES-ZsGreen-W
Plasmid#164432PurposeLentiviral vector backbone encoding firefly luciferase and ZsGreenDepositorInsertsLuciferase
ZsGreen
UseLentiviralAvailable SinceFeb. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGV15 (CK2alpha K68M)
Plasmid#27089DepositorInsertCK2alpha K68M (kinase dead) (CSNK2A1 Human)
TagsHAExpressionMammalianMutationK68M (kinase dead)PromoterCMVAvailable SinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbOcIgG-Tn5
Plasmid#184285PurposeAnti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbOcIgG-Tn5
Available SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP703-N1
Plasmid#136558PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertemiRFP703
ExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only
