4,147 results

1. pAc5.1B-EGFP

   Plasmid

   #21181

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

   Insert

   enhanced green flourescent protein
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMVR8.74

   Plasmid

   #22036

   Purpose

   2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Insert

   gag pol tat rev
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-KG-D4H*-mCherry

   Plasmid

   #134604

   Purpose

   fluorescent cholesterol-binding probe

   Depositor

   Roberto Zoncu

   Article

   Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

   Insert

   D4H*
   

   Use

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   Y415A and A463W

   Promoter

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSIP403

   Plasmid

   #122028

   Purpose

   Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

   Depositor

   Geir Mathiesen, Lars Axelsson

   Article

   Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

   Insert

   gusA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sppA

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-4T1 SHP2 WT

   Plasmid

   #8322

   Depositor

   Ben Neel

   Article

   O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-EGFP

   Plasmid

   #89684

   Purpose

   pCAG promoter driving EGFP

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA

   Plasmid

   #84474

   Purpose

   pAAV-RAM-NLS-mKate2

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   mKate2
   

   Use

   AAV

   Tags

   Nuclear localization signal of SV40 large T antig…

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR-SFFV-dCas9-BFP-KRAB

   Plasmid

   #46911

   Purpose

   Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-BFP-KRAB fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2xNLS, BFP, HA, and KRAB domain

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET MBP His6 LIC cloning vector (2Cc-T)

   Plasmid

   #37237

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PB_tre_Cas9

    Plasmid

    #126029

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible Cas9

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    humanized S. pyrogenes Cas9
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha and TRE

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CROP-seq-opti

    Plasmid

    #106280

    Purpose

    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAVS1-NC-CRISPRi (Gen3)

    Plasmid

    #73499

    Purpose

    Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAVS1-TLR targeting vector

    Plasmid

    #64215

    Purpose

    Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    traffic light reporter (TLR) (AAVS1 Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    see publication for details

    Promoter

    CAG

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDx_mScarlet-I3

    Plasmid

    #189757

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV & Rha

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. myrAkt delta4-129

    Plasmid

    #10841

    Depositor

    Richard Roth

    Article

    Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

    Insert

    Akt (AKT1 Human)
    

    Use

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    Deletion of PH domain (aa4-129)

    Promoter

    SV40

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2-MT GLI2 delta N

    Plasmid

    #17649

    Depositor

    Erich Roessler

    Article

    Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

    Insert

    GLI2 (GLI2 Human)
    

    Use

    Tags

    6x Myc

    Expression

    Mammalian

    Mutation

    Missing 328 N-terminal amino acids

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVax1-hVEGF165

    Plasmid

    #74466

    Purpose

    expression of hVEGF165 in mammalian cells

    Depositor

    Loree Heller

    Article

    Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

    Insert

    vascular endothelial growth factor 165 (VEGFA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX333

    Plasmid

    #64073

    Purpose

    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    3XFLAG-Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CBh; U6

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCW57.1-FOXM1b

    Plasmid

    #68811

    Purpose

    Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells

    Depositor

    Adam Karpf

    Article

    Barger et al Oncotarget. 2015 Jul 16.

    Insert

    FOXM1b (FOXM1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFLAG-YAP1

    Plasmid

    #66853

    Purpose

    Express FLAG-tagged YAP1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LRG2.1

    Plasmid

    #108098

    Purpose

    Lentiviral expression plasmid of sgRNA with GFP

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBMN-I-GFP

    Plasmid

    #1736

    Purpose

    This retroviral vector has an IRES-GFP downstream of the cloning sites

    Depositor

    Garry Nolan

    Insert

    IRES-GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Flag-SENP1

    Plasmid

    #17357

    Depositor

    Edward Yeh

    Article

    Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

    Insert

    SENP1 (SENP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA-3xHA-hTERT

    Plasmid

    #51637

    Purpose

    Mammalian expression of 3xHA tagged human TERT, N-terminal.

    Depositor

    Steven Artandi

    Article

    Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

    Insert

    TERT (TERT Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p53-GFP

    Plasmid

    #11770

    Depositor

    Geoff Wahl

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    72R variant of p53

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. eGFP STAT1 S727A

    Plasmid

    #12304

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

    Insert

    STAT1 S727A (STAT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S727A

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. gRNA_Cloning Vector

    Plasmid

    #41824

    Purpose

    An empty gRNA expression vector, used to create a gRNA to a specific sequence.

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    AflII site for Gibson Assembly

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-SypHluorin 4x (S4x)

    Plasmid

    #37005

    Depositor

    Yongling Zhu, Stephen Heinemann

    Article

    Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

    Insert

    SypHluorin 4x (Syp Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Four copies of pHluorin inserted at site 184-185

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hSyn-hM3D(Gq)-mCherry

    Plasmid

    #50474

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. phage UbiC G3BP1-GFP-GFP

    Plasmid

    #119950

    Purpose

    Lentiviral vector expressing G3BP1-GFP fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge