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169,512 results

Showing: 1851 - 1900 of 169512 results

  4. pRSET-iGluSnFR4s

    Plasmid
    #234443
    Purpose
    Bacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow)
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s
    Tags
    6xHis-Xpress epi
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234443-plasmid-map-sequence-id-464951

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  7. pLKO.1-blast shGFP

    Plasmid
    #110419
    Purpose
    shGFP control, silence GFP gene, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GFP
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 (RNA PolIII)
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110419-plasmid-map-sequence-id-227288

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  11. mMaroon-N1

    Plasmid
    #54554
    Purpose
    Localization: N1 Cloning Vector, Excitation: 610, Emission: 652
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mMaroon
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54554-plasmid-map-sequence-id-475388

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  12. pCDH-IFNA-neo

    Plasmid
    #225710
    Purpose
    The pCDH-IFNA-neo plasmid is designed for the overexpression of human IFNα in mammalian cells. This plasmid was utilized to engineer A549 cells for stable IFNα overexpression.
    Depositor
    Eun Hee Han
    Article
    Min et al Commun Biol. 2024 Sep 30;7(1):1222. doi:
    Insert
    IFNA (IFNA1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225710-plasmid-map-sequence-id-448635

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  20. pCDH-EF1-N-cG-Neo

    Plasmid
    #124978
    Purpose
    Express Nanoluc, copGFP
    Depositor
    Kazuhiro Oka
    Insert
    Nanoluc, copGFP
    Use
    Lentiviral
    Promoter
    EF1-alpha
    Available Since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    124978-plasmid-map-sequence-id-243972

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  22. ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

    Plasmid
    #187459
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187459-plasmid-map-sequence-id-367469

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  24. pY116 (pcDNA3.1-huMb2Cpf1)

    Plasmid
    #92292
    Purpose
    Expression of humanized Mb2Cpf1
    Depositor
    Feng Zhang
    Insert
    huMb2Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available Since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92292-plasmid-map-sequence-id-181491

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  30. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

    Plasmid
    #225947
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    S3E S17E S19E
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225947-plasmid-map-sequence-id-446849

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  31. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

    Plasmid
    #225948
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    S3A S17A S19A
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225948-plasmid-map-sequence-id-446850

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  38. MRGPRX4-Tango

    Plasmid
    #66442
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MRGPRX4 (MRGPRX4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  41. MRGPRE-Tango

    Plasmid
    #66436
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MRGPRE (MRGPRE Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  43. pJYDN3p

    Plasmid
    #162457
    Purpose
    Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA and myc tags, and eUnaG2 fluorescent…
    Promoter
    GAL1
    Available Since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162457-plasmid-map-sequence-id-321472

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  48. GCN5 core-dCas9-CBP core

    Plasmid
    #179555
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179555-plasmid-map-sequence-id-354784

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Showing: 1851 - 1900 of 169512 results