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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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19,159 results

Showing: 2001 - 2050 of 19159 results

  1. FSW_jGCaMP8f

    Plasmid
    #197034
    Purpose
    Express GCaMP8f from hSyn1 promoter
    Depositor
    Ronald Hart
    Insert
    GCaMP8f
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197034-plasmid-map-sequence-id-392710

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  2. pIMAY

    Plasmid
    #68939
    Purpose
    E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange
    Depositor
    Tim Foster
    Article
    Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.
    Type
    Empty backbone
    Use
    Staphylococcal allelic exchange vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68939-plasmid-map-sequence-id-210239

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  14. LV_EF1a_SV40_Large_T_Antigen-P2A-Hygro_Barcode

    Plasmid
    #170255
    Purpose
    Barcoded lentiviral vector to express SV40 Large T Antigen in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    SV40 Large T Antigen
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170255-plasmid-map-sequence-id-334417

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  18. pSCAR_Cas9-blast_GFP

    Plasmid
    #162074
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-blasticidin S deaminase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162074-plasmid-map-sequence-id-471574

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  20. kiCAP-AAV-BI30

    Plasmid
    #183749
    Purpose
    AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.
    Depositor
    Ben Deverman
    Article
    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40
    Insert
    AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
    Use
    AAV
    Tags
    Expression
    Mutation
    K449R, and NNSTRGG inserted between 588 and 589 o…
    Promoter
    p41
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    183749-plasmid-map-sequence-id-442496

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  25. bZiPro

    Plasmid
    #86846
    Purpose
    express NS2B-NS3 protease in E.coli
    Depositor
    Dahai Luo
    Article
    Zhang et al Science. 2016 Dec 8. pii: aai9309.
    Inserts
    NS3 1-177
    NS2B45-96
    Use
    Tags
    a hexahistidine tag and a thrombin cleavage site
    Expression
    Bacterial
    Mutation
    NS2B cofactor region (residues 45–96) and NS3 pro…
    Promoter
    T7
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86846-plasmid-map-sequence-id-172845

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  26. HOP-flash

    Plasmid
    #83467
    Purpose
    TEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter gene
    Depositor
    Barry Gumbiner
    Article
    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d
    Insert
    Multimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83467-plasmid-map-sequence-id-350804

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  34. pSBbi-Bla

    Plasmid
    #60526
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60526-plasmid-map-sequence-id-97520

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  35. pET22b_PstS_1

    Plasmid
    #78198
    Purpose
    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A197C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A197C (please see depositors comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78198-plasmid-map-sequence-id-146048

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  37. pLX_EFS-Cre_ppt-del

    Plasmid
    #162073
    Purpose
    Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Insert
    Cre
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EFS (part of insert)
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162073-plasmid-map-sequence-id-325292

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  38. pCMV-PE6b

    Plasmid
    #207852
    Purpose
    Mammalian expression of PE6b prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6b
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207852-plasmid-map-sequence-id-408170

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Showing: 2001 - 2050 of 19159 results