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Showing: 2281 - 2320 of 158569 results

  5. pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)

    Plasmid
    #44095
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44095-plasmid-map-sequence-id-61672

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  7. pD649-HAsp-IL1R1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156756
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IL1R1 (IL1R1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156756-plasmid-map-sequence-id-404140

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  10. TFORF1522

    Plasmid
    #143288
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NR2E3 (NR2E3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143288-plasmid-map-sequence-id-396141

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  11. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

    Plasmid
    #89888
    Purpose
    Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d
    Insert
    mCherry-KRasG12V
    Use
    Unspecified
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89888-plasmid-map-sequence-id-181093

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  14. pBMN HA

    Plasmid
    #188645
    Purpose
    This is the Lazarou lab's standard retrovirus vector backbone for expression of HA-tag fusions onto the N-terminus of cloned genes
    Depositor
    Michael Lazarou
    Article
    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    188645-plasmid-map-sequence-id-370206

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  16. SETDB1

    Plasmid
    #25240
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETDB1, SETDB1_16 (SETDB1 Human)
    Use
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pLpA-DN.JNK2

    Plasmid
    #51943
    Purpose
    Adenoviral vector for dominant negative JNK2 expression
    Depositor
    Jeffery Molkentin
    Article
    Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.
    Insert
    DN JNK2 (Mapk9 Mouse)
    Use
    Adenoviral
    Tags
    HA
    Expression
    Mutation
    dominant negative: the dual phosphorylation moti…
    Promoter
    CMV
    Available since
    May 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pBW001

    Plasmid
    #131529
    Purpose
    Vector containing the Mu-BsaI-KanR transposon that can be excised with HindIII and BglII for transposition reactions
    Depositor
    Jonathan Silberg
    Article
    Wu et al bioRxiv May 23, 2019
    Insert
    Mu-BsaI-KanR transposon
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    131529-plasmid-map-sequence-id-263309

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  29. pBbB8k-csg-nb112

    Plasmid
    #171702
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1
    Insert
    csgBACEFG, with nanobody fused to CsgA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171702-plasmid-map-sequence-id-336472

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  33. pKEK3394

    Plasmid
    #234078
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    tspurple
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    May 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234078-plasmid-map-sequence-id-472134

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  34. 8061-E33

    Plasmid
    #234270
    Purpose
    Gateway ORF Entry clone of mouse Smad3 with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    Smad3 (Smad3 Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234270-plasmid-map-sequence-id-468854

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  36. pKEK3238

    Plasmid
    #234092
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfCherry2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234092-plasmid-map-sequence-id-467988

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  37. pKEK3228

    Plasmid
    #234090
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    aeBlue
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234090-plasmid-map-sequence-id-467993

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  38. pKEK3227

    Plasmid
    #234089
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    YukonOFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234089-plasmid-map-sequence-id-467986

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Showing: 2281 - 2320 of 158569 results