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159,258 results

Showing: 2951 - 3000 of 159258 results

  2. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Promoter
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

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  11. pAAVone-AAV9-CMV-eGFP

    Plasmid
    #230928
    Purpose
    pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.
    Depositor
    Qizhao Wang
    Insert
    eGFP
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230928-plasmid-map-sequence-id-458358

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  18. 10TALE_Pmin_fLuc

    Plasmid
    #160663
    Purpose
    Expression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domain
    Depositor
    Roman Jerala
    Article
    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20
    Insert
    10TALE_Pmin_fLuc
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    minimal
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160663-plasmid-map-sequence-id-345378

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  21. pH6-MBP-TEV-BirA

    Plasmid
    #179694
    Purpose
    Expresses His6 tagged MBP-BirA fusion protein
    Depositor
    Mark Arbing
    Insert
    BirA
    Use
    Tags
    His6-MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179694-plasmid-map-sequence-id-352195

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  24. pcDNA3-CD14

    Plasmid
    #13645
    Depositor
    Doug Golenbock
    Insert
    CD14 (CD14 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13645-plasmid-map-sequence-id-7723

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  32. pDisCoTune

    Plasmid
    #173713
    Purpose
    Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.
    Depositor
    Morten Norholm
    Article
    Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2
    Inserts
    ERV1 (ERV1 Budding Yeast)
    PDI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    173713-plasmid-map-sequence-id-342928

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  33. CMV mRuby3-Synapsin1a

    Plasmid
    #187896
    Purpose
    For labeling pre-synaptic terminals
    Depositor
    Timothy Ryan
    Insert
    Synapsin1a
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187896-plasmid-map-sequence-id-394752

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  36. hNFAT2-409:TALE:VP64:KRɸ

    Plasmid
    #160661
    Purpose
    Constitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.
    Depositor
    Roman Jerala
    Article
    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20
    Insert
    hNFAT2-409:TALE:VP64:KRɸ
    Use
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    160661-plasmid-map-sequence-id-338890

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  38. mCherry-Ezrin-N-14

    Plasmid
    #55043
    Purpose
    Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Ezrin (EZR Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55043-plasmid-map-sequence-id-271041

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  41. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

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  46. PB-TO-ASCL1-DLX2

    Plasmid
    #182307
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Inserts
    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and pTRE3G-bidirectional
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182307-plasmid-map-sequence-id-359517

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  48. EGFP-Vimentin-7

    Plasmid
    #56439
    Purpose
    Localization: Intermediate Filaments, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56439-plasmid-map-sequence-id-323617

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Showing: 2951 - 3000 of 159258 results