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Showing: 2951 - 3000 of 157452 results

  1. pHEE-R-bar9si

    Plasmid
    #222834
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, bialaphos and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222834-plasmid-map-sequence-id-446485

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  2. pD649-HAsp-PDCD1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156543
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    PDCD1 (PDCD1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    156543-plasmid-map-sequence-id-368511

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  3. tdTomato-LaminB1-10

    Plasmid
    #58107
    Purpose
    Localization: Nuclear Envelope, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    LaminB1 (LMNB1 Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    58107-plasmid-map-sequence-id-393556

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  6. pCDH-DCX-TurboID-V5-puro

    Plasmid
    #190742
    Purpose
    A lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)
    Depositor
    Jeremy Baskin
    Article
    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021
    Insert
    DCX-TurboID-V5 (DCX DCX is human doublecortin; TurboID is engineered BirA from E.coli, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    190742-plasmid-map-sequence-id-379321

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  7. p405GAL1

    Plasmid
    #15963
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    p405GAL1 (GAL1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    GAL1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15963-plasmid-map-sequence-id-8448

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  18. pLV-mRFP1-Human UTROPHIN_1-261

    Plasmid
    #225052
    Purpose
    Express in mammalian cells the mRFP1 fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.
    Depositor
    Pantelis Tsoulfas
    Insert
    Human Utrophin residues 1-261 and RFP1 (UTRN Homo Sapiens, Human)
    Use
    Lentiviral
    Tags
    Human UTROPHIN 1-261 and mRFP1
    Expression
    Mammalian
    Mutation
    Sequence is derived from ADDGENE Plasmid #26739-R…
    Promoter
    CMV promoter and enhancer
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    225052-plasmid-map-sequence-id-446521

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  19. pLV-EGFP-Human-UTROPHIN_1-261

    Plasmid
    #222393
    Purpose
    Express in mammalian cells the EGFP fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.
    Depositor
    Pantelis Tsoulfas
    Insert
    Human Utrophin residues 1-261 and EGFP (UTRN Homo Sapiens, Human)
    Use
    Lentiviral
    Tags
    EGFP and Human UTROPHIN 1-261
    Expression
    Mammalian
    Mutation
    Sequence is derived from ADDGENE Plasmid #26737-G…
    Promoter
    CMV promoter and enhancer
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    222393-plasmid-map-sequence-id-446518

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  22. pJA48 HLA-B*57:01

    Plasmid
    #180444
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*57:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    180444-plasmid-map-sequence-id-366471

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  25. pSAP

    Plasmid
    #206429
    Purpose
    A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    206429-plasmid-map-sequence-id-419733

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  29. TFORF1069

    Plasmid
    #143463
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFATC2 (NFATC2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143463-plasmid-map-sequence-id-397913

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  30. pTCF5 HLA-A*01:01

    Plasmid
    #180448
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*01:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    180448-plasmid-map-sequence-id-355109

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  31. pTCF37 HLA-B*35:01

    Plasmid
    #180450
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*35:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    180450-plasmid-map-sequence-id-358091

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  34. mCherry-CDC42-C-10

    Plasmid
    #55014
    Purpose
    Localization: Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    CDC42 (CDC42 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid
    #75154
    Purpose
    Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    H2B-mCerulean3-P2A-mKOFP2-CAAX
    Use
    Middle entry vector for multisite gateway three f…
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75154-plasmid-map-sequence-id-142108

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  40. TFORF0722

    Plasmid
    #141673
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFKB1 (NFKB1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141673-plasmid-map-sequence-id-404053

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  45. pETM33_Nsp15

    Plasmid
    #156481
    Purpose
    Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp15 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156481-plasmid-map-sequence-id-307733

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  46. pD649-HAsp-CD274-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156660
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CD274 (CD274 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156660-plasmid-map-sequence-id-410005

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Showing: 2951 - 3000 of 157452 results