19,167 results

1. pCas9-sgAAVS1-1

   Plasmid

   #129726

   Purpose

   Expressing SpCas9 and sgAAVS1-1

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   SpCas9 and sgAAVS1-1
   

   Use

   CRISPR

   Tags

   P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lenti-EF1a-dCas9-KRAB-Puro

   Plasmid

   #99372

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-KRAB-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21a-alpha-synuclein

   Plasmid

   #51486

   Purpose

   Inducible expression of human alpha-synuclein in E.coli

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 31, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pcDNA3.1+-Tornado-nLuc

   Plasmid

   #212611

   Purpose

   For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   Tornado-CVB3-nLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG32

   Plasmid

   #35122

   Depositor

   John McCusker

   Article

   Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

   Insert

   hphMX4
   

   Use

   Yeast replicating

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQE30-His-AsnRS

   Plasmid

   #124105

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Asparaginyl-tRNA ligase (asnS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET16b-His-ArgRS

   Plasmid

   #124104

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Argin+I4 ligase (argS Escherichia coli)
   

   Use

   Tags

   10x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBAM1

   Plasmid

   #60487

   Purpose

   This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome

   Depositor

   Víctor de Lorenzo

   Article

   Martinez-Garcia et al BMC Microbiol. 2011 Feb 22;11:38. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB-TA-ERP2

   Plasmid

   #80477

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRK5M-ADAM10

    Plasmid

    #31717

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQE30-His-PheRS

    Plasmid

    #124116

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RpHLuorin2

    Plasmid

    #180227

    Purpose

    for bacterial expression of recombinant ratiometric pHluorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2
    

    Use

    Tags

    His-tag followed by thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FKBP-V5-AP-nes_pLX304

    Plasmid

    #120912

    Purpose

    Lentiviral vector expressing FKBP-V5-AP-NES in mammalian cytosol

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    FKBP-V5-AP-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IKK-2 S177E S181E

    Plasmid

    #11105

    Purpose

    Mammalian expression of constitutively active human IKK-2

    Depositor

    Anjana Rao

    Article

    Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

    Insert

    IKK-2 (IKBKB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S177E and S181E, constitutively active

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21a-MetRS-His

    Plasmid

    #124115

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Methionine--tRNA ligase (metG Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ARR3tk-eGFP/SV40-mCherry

    Plasmid

    #132360

    Purpose

    To measure transcriptional activity of Androgen Receptor

    Depositor

    Charles Sawyers

    Article

    Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

    Insert

    AR responsive elements (AR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40

    Plasmid

    #105540

    Purpose

    AAV expression of EGFP-Cre from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP-Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAS_4xMma PylT_GFP 150TAG 6xHis

    Plasmid

    #140015

    Purpose

    Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lenti sgRNA(MS2)_puro backbone

    Plasmid

    #73795

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-TLR4-YFP

    Plasmid

    #13018

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 4 (TLR4 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mApple-Alpha-V-Integrin-25

    Plasmid

    #54866

    Purpose

    Localization: Focal Adhesions, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Alpha-V Integrin (ITGAV Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUA

    Plasmid

    #58372

    Purpose

    UAS vector to express gene of interest in Drosophila

    Depositor

    Chun Han, Yuh-Nung Jan

    Article

    Han et al Neuron. 2014 Feb 5;81(3):544-60. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDH-EF1a-eFFly-eGFP

    Plasmid

    #104834

    Purpose

    eFFly luciferase for invivo Imaging, eGFP for flourescent detection

    Depositor

    Irmela Jeremias

    Article

    Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

    Inserts

    eFFly
    eGFP
    

    Use

    Lentiviral and Luciferase; Egfp

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLVX-CMV-Galpha-s ONE-GO

    Plasmid

    #189731

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-s ONE-GO
    GNAS (GNAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET21a-AspRS-His

    Plasmid

    #124106

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Aspartate-tRNA ligase (aspS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-mCherry-Mango II x 24

    Plasmid

    #127587

    Purpose

    Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

    Depositor

    David Rueda

    Article

    Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

    Insert

    mCherry-Mango II x24
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE Tet ON

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3 CB1

    Plasmid

    #13391

    Purpose

    Expresses mouse cannabinoid receptor 1 (CB1) in mammalian cells

    Depositor

    Mary Abood

    Article

    Abood et al Biochem Pharmacol. 1997 Jan 24. 53(2):20

    Insert

    CB1 (Cnr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Cys-3HB-SPYcatcher

    Plasmid

    #214838

    Purpose

    This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Cys-3HB-SPYcatcher
    

    Use

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p5E-elavl3

    Plasmid

    #75025

    Purpose

    Can be used to drive pan neuronal expression in zebrafish.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    elavl3

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. phage UbiC G3BP1-GFP-GFP

    Plasmid

    #119950

    Purpose

    Lentiviral vector expressing G3BP1-GFP fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pQE30-His-ThrRS

    Plasmid

    #124119

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Threonyl-tRNA ligase (thrS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET21a-His-IleRS

    Plasmid

    #124112

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Isoleucyl-tRNA ligase (ileS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDARMO_HUWE1_3xFLAG

    Plasmid

    #187155

    Purpose

    mammalian cell expression of HUWE1-3xFLAG

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLY017SB_pAAV-U6sg(BbsI)-EFS-Thy1.1-P2A-SB100X

    Plasmid

    #192151

    Purpose

    T cell CRISPR AAV-SB vector

    Depositor

    Sidi Chen

    Article

    Ye et al Nat Biotechnol. 2019 Nov;37(11):1302-131

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET21a-LeuRS-His

    Plasmid

    #124113

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Leucyl-tRNA ligase (leuS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.3_d2eGFP

    Plasmid

    #26821

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Sep 29. ():.

    Insert

    5'UTR-d2eGFP-3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    destabilized (PEST sequence) GFP

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. FRP880_PACT1(-1-520)-LexA-ER-haB112-TCYC1

    Plasmid

    #58437

    Purpose

    Expresses LexA-ER-B112 in yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    LexA-ER-B112
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Act1 promoter (-520;1)

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET21a-CysRS-His

    Plasmid

    #124107

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Cysteinyl-tRNA ligase (cysS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET21a-GlyRS-His

    Plasmid

    #124110

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Lyn-tailed mCherry-SEpHluorin

    Plasmid

    #32002

    Depositor

    Sergio Grinstein

    Article

    Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

    Insert

    Membrane-targeting sequence of Lyn kinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET21a-ProRS-His

    Plasmid

    #124117

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Prolyl-tRNA ligase (proS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET21a-TrpRS-His

    Plasmid

    #124120

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Tryptophanyl-tRNA ligase (trpS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCSCX

    Plasmid

    #63918

    Purpose

    Expression of the sucrose utilization phenotype

    Depositor

    Claudia Vickers

    Article

    Bruschi et al Biotechnol Adv. 2012 Sep-Oct;30(5):1001-

    Insert

    cscAKB
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET21a-HisRS-His

    Plasmid

    #124111

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Histidyl-tRNA ligase (hisS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLKO5.sgRNA.EFS.PAC

    Plasmid

    #57825

    Purpose

    Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCAG mito-GCaMP5G

    Plasmid

    #105009

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    GCaMP5G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pLifeAct_mScarlet-i_N1

    Plasmid

    #85056

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pmRFP-LC3

    Plasmid

    #21075

    Purpose

    Mammalian expression of rat LC3 fused to mRFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kimura et al Autophagy. 2007;3(5):452-60.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    First ATG is not included.

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pEGFP Kindlin2

    Plasmid

    #105305

    Purpose

    To study effect of Kindlin 2 on Cell/ECM adhesion

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pGWB540

    Plasmid

    #74874

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge