Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

169,589 results

Showing: 3301 - 3350 of 169589 results

  2. pSCAR_Cas9-blast_GFP

    Plasmid
    #162074
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-blasticidin S deaminase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available Since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162074-plasmid-map-sequence-id-471574

    Enlarge

  22. pCMVtrunc msfGFPΔC9-LifeactΔN2

    Plasmid
    #231556
    Purpose
    Mammalian expression of N-terminally truncated Lifeact fused to C-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Lifeact (ABP140 Budding Yeast)
    Tags
    msfGFP
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231556-plasmid-map-sequence-id-459689

    Enlarge

  25. pRepCap6

    Plasmid
    #110770
    Purpose
    pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.
    Depositor
    David Russell
    Article
    Rutledge et al J Virol. 1998 Jan;72(1):309-19.
    Insert
    AAV6 cap, AAV6 rep genes
    Use
    AAV
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110770-plasmid-map-sequence-id-211461

    Enlarge

  29. R777-E317 Hs.SOS1

    Plasmid
    #70601
    Purpose
    Gateway ORF clone of human SOS1 [NM_005633.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SOS1 (SOS1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70601-plasmid-map-sequence-id-358354

    Enlarge

  34. MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

    Plasmid
    #176624
    Purpose
    TF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3
    Depositor
    Michael Elowitz
    Article
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
    Use
    Synthetic Biology
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176624-plasmid-map-sequence-id-347134

    Enlarge

  35. DVC_p15A_AF

    Plasmid
    #217588
    Purpose
    Destination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.
    Depositor
    Wei Huang
    Article
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    None
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217588-plasmid-map-sequence-id-442568

    Enlarge

  40. TFORF1497

    Plasmid
    #143126
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LYL1 (LYL1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143126-plasmid-map-sequence-id-397859

    Enlarge

  47. pET-pAG-Hia5

    Plasmid
    #174373
    Purpose
    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein AG - hia5
    Tags
    6xHis and Protein A/G
    Expression
    Bacterial
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174373-plasmid-map-sequence-id-341861

    Enlarge

Showing: 3301 - 3350 of 169589 results