225,450 results
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OaAEP1_C247A
Plasmid#200307PurposeBacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)DepositorInsertAsparaginyl endopeptidase from Oldenlandia affinis
TagsSmt3ExpressionBacterialMutationCysteine 247 to AlaninePromoterT7Available SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.Cre.WPRE.hGH (AAV8)
Viral Prep#105553-AAV8PurposeReady-to-use AAV8 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYL192 (TRV1)
Plasmid#148968PurposeTobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)DepositorInsertTRV RNA1
UsePlant vector with trv rna1; this vector used with…MutationP697S mutation in putative replicase. S239G and S…Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pSGDlucV3.0
Plasmid#119760Purposedual luciferase with FMDV StopGo sequences flanking MCSDepositorTypeEmpty backboneUseLuciferaseExpressionMammalianPromoterSV40 and T7Available SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Popt
Plasmid#231705PurposeMammalian expression vector encoding a codon-optimized P gene from vesicular stomatitis virus, strain Indiana.DepositorInsertVSV P gene (codon-optimized )
UseVsv expressionExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Lopt
Plasmid#231706PurposeMammalian expression vector encoding a codon-optimized L gene from vesicular stomatitis virus, strain Indiana.DepositorInsertVSV L gene (codon-optimized )
UseVsv expressionExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAF0687 Pa01_attP-mCherry donor
Plasmid#193466PurposePromoterless mcherry donor for Pa01DepositorInsertPa01_attP
ExpressionMammalianAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGreenIIM DR5v2-ntdTomato/DR5-n3GFP
Plasmid#61628PurposeExpresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoterDepositorInsertsntdTomato
n2xEGFP
ExpressionPlantMutation2 copies of EGFP with N-terminal SV40 NLSPromoterAuxin-dependent DR5 promoter containing 9 reverse…Available SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGreenIIM DR5v2-ntdTomato/DR5-n3GFP
Plasmid#61628PurposeExpresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoterDepositorInsertsntdTomato
n2xEGFP
ExpressionPlantMutation2 copies of EGFP with N-terminal SV40 NLSPromoterAuxin-dependent DR5 promoter containing 9 reverse…Available SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)
Plasmid#182287PurposeEncodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
Tagsnuclear export signal (NES)ExpressionMammalianMutationY306A/Y384F (AF) double mutant of Methanosarcina …PromoterCMV and U6Available SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER11 (miR-RUG)
Plasmid#44363PurposeInducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interestDepositorInsertPheS Gly294
UseLentiviralAvailable SinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER11 (miR-RUG)
Plasmid#44363PurposeInducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interestDepositorInsertPheS Gly294
UseLentiviralAvailable SinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCnRed
Plasmid#232908PurposeElectroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator.DepositorAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEf1a-goldDn29-P2A-GFP
Plasmid#247153PurposeMammalian expression of a human codon optimized engineered goldDn29 recombinase (efficient and specific variant)DepositorInsertgoldDn29-P2A-GFP
Tags6xHisExpressionMammalianMutationM6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503NPromoterEf1aAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pICH47742:: pRPS5a-PE2max-NC::tHSP
Plasmid#227694PurposeGolden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.DepositorInsertPE2max-NC being driven by pRPS5a and terminated by tHSP
UseCRISPRExpressionPlantPromoterpRPS5aAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY0423 Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)
Plasmid#179116PurposeCargo EGFP with AttP Bxb1 site for in-frame fusion (Parent minicircle)DepositorInsertCargo EGFP with AttP Bxb1 site for in-frame fusion
UseCRISPRAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE
Plasmid#139090PurposeExpresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertdSpCas9-VPR
UseCRISPR and LentiviralExpressionMammalianMutationD10A, H840AAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH2.22_mCherry-MS2x8
Plasmid#205537PurposeExpress mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
ExpressionMammalianMutationWTPromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMG36E
Plasmid#133853PurposeExpression vectorDepositorTypeEmpty backboneExpressionBacterialPromoterP32Available SinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Nopt
Plasmid#231704PurposeMammalian expression vector encoding a codon-optimized N gene from vesicular stomatitis virus, strain Indiana.DepositorInsertVSV N gene (codon-optimized ) (VSIVgp1 vesicular stomatitis virus)
UseVsv expressionExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDY0750 pCAG-NLS-BceINT
Plasmid#179112PurposeBceINT expression vectorDepositorInsertpCAG-NLS-BceINT
UseCRISPRAvailable SinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ZFN-AAVS1
Plasmid#89707PurposeMammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locusDepositorInsertZFN-AAVS1
ExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY-eRF1(E55D)
Plasmid#157925PurposeEncodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertPOLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
ExpressionMammalianPromoterCMVAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES2_EGFP_KCNQ1
Plasmid#173161PurposeFor mammalian expression of KCNQ1 and creating variantsDepositorAvailable SinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM461
Plasmid#108320PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacBDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
SacRB
ExpressionBacterialAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-VAPB
Plasmid#108126PurposeER markerDepositorAvailable SinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
P307-FBP_6
Plasmid#139697PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathwayDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.S
Plasmid#103006Purposenon-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.S VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
EJ2GFP-puro
Plasmid#44025DepositorInsertEJ2GFP egfp-based chromosomal break reporter
ExpressionMammalianMutationinsertion of I-SceI, 3 frame stop, and flanking m…PromoterpCAGGSAvailable SinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
EJ2GFP-puro
Plasmid#44025DepositorInsertEJ2GFP egfp-based chromosomal break reporter
ExpressionMammalianMutationinsertion of I-SceI, 3 frame stop, and flanking m…PromoterpCAGGSAvailable SinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-EGFP (AAV8)
Viral Prep#50457-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-GFP-Gal8
Plasmid#127191PurposePiggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.DepositorInsertGFP-Gal8 (LGALS8 Human)
TagsGal8 is fused to the c-terminus of GFPExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
TUBB5-Halo
Plasmid#64691PurposeExpresses beta-tubulin labeled with Halo-tagDepositorInsertTUBB
TagsHalo-tagExpressionMammalianAvailable SinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
TUBB5-Halo
Plasmid#64691PurposeExpresses beta-tubulin labeled with Halo-tagDepositorInsertTUBB
TagsHalo-tagExpressionMammalianAvailable SinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-N7HELIX-KanR
Plasmid#190663PurposeKanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequenceDepositorInsertKanR, R6K origin of replication
ExpressionBacterialPromoterNAAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_hsTRPA1_myc_dTom
Plasmid#183179Purposemyc tagged hsTRPA1 used for cell transfectionDepositorInsertsExpressionMammalianAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS307
Plasmid#51785Purposean integrating (YIp) vector with a LYS2 selectable markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS307
Plasmid#51785Purposean integrating (YIp) vector with a LYS2 selectable markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2FE-ABE8e-SpRY
Plasmid#213008PurposeA lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning siteDepositorInsertABE8e-SpRY-D10A
UseCRISPR and LentiviralExpressionMammalianMutationD10A nickase variant of SpRYPromoterEf-1aAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Nrf2
Plasmid#67636PurposeAAV vector expressing Nrf2 geneDepositorAvailable SinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Nrf2
Plasmid#67636PurposeAAV vector expressing Nrf2 geneDepositorAvailable SinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo
Plasmid#139449PurposeLentiviral vector with gRNA scaffold and neomycin selectable markerDepositorInsertno sgRNA inserted; resistance gene: neoR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pN7CAST
Plasmid#190661PurposeExpresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRESDepositorInserthuman codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
ExpressionMammalianPromoterCMVAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRH030
Plasmid#159633PurposeCRISPR‐Cas9 tool for the genetic engineering of Bacillus phagesDepositorTypeEmpty backboneUseCRISPR; Shuttle vectorPromoterConstitutive tracrRNAAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only
