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20,804 results

Showing: 6351 - 6400 of 20804 results

  1. PTGER4-Tango

    Plasmid
    #66486
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    PTGER4 (PTGER4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66486-plasmid-map-sequence-id-276279

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  17. pAB2076 pAAV REPAIR.t1

    Plasmid
    #176323
    Purpose
    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    Use
    AAV and CRISPR
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    EFS (short EF1alpha) and hU6
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176323-plasmid-map-sequence-id-345286

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  25. Tandem p40p35

    Plasmid
    #108665
    Purpose
    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker
    Depositor
    Nevil Singh
    Insert
    interleukin-12 (Il12a, Il12b Mouse)
    Expression
    Mammalian
    Mutation
    IL-12 dimers have a Gly-Ser linker
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    108665-plasmid-map-sequence-id-260875

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  30. Hygro-Cas9 donor

    Plasmid
    #86883
    Purpose
    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection
    Depositor
    Danwei Huangfu
    Article
    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86883-plasmid-map-sequence-id-173280

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  35. GreenGate 2.0 Toolkit

    Plasmid Kit
    #1000000241
    Purpose
    The GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.
    Depositor
    Alexis Maizel
    Available Since
    April 11, 2024
    Availability
    Academic Institutions and Nonprofits only

  39. pMAL-c5x_UnaG

    Plasmid
    #163125
    Purpose
    A plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.
    Depositor
    Julie Biteen
    Article
    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi
    Insert
    UnaG
    Tags
    His and MBP
    Expression
    Bacterial
    Available Since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    163125-plasmid-map-sequence-id-324911

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  40. pAAV-nEF-ChRmine-mScarlet (AAV8)

    Viral Prep
    #137158-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-ChRmine-mScarlet (#137158). In addition to the viral particles, you will also receive purified pAAV-nEF-ChRmine-mScarlet plasmid DNA. nEF-driven expression of ChRmine-mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    mScarlet
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only

  41. pCAG-NLS-HA-Dre

    Plasmid
    #51272
    Purpose
    Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)
    Depositor
    Pawel Pelczar
    Article
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    Dre
    Tags
    NLS-HA
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51272-plasmid-map-sequence-id-334322

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  42. pMT-puro

    Plasmid
    #17923
    Purpose
    Insect expression vector
    Depositor
    David Sabatini
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Drosophila metallothionein gene promoter drives e…
    Expression
    Insect
    Available Since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    17923-plasmid-map-sequence-id-211593

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  43. pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 (AAV1)

    Viral Prep
    #101061-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 (#101061). In addition to the viral particles, you will also receive purified pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator his-tagged CaMPARI2-F391W. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Eric Schreiter
    Article
    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:
    Promoter
    Syn
    Available Since
    July 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  50. The Mammalian Toolkit

    Plasmid Kit
    #1000000180
    Purpose
    A ready-made extensive library of plasmids that enable Golden Gate high-throughput assembly of genetic circuits that can be delivered to cells in a multitude of ways.
    Depositor
    Hana El-Samad
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 6351 - 6400 of 20804 results