Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

171,784 results

Showing: 7601 - 7650 of 171784 results

  1. pAAV-CAG-tdTomato (codon diversified) (AAV5)

    Viral Prep
    #59462-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-CAG-tdTomato (codon diversified) (#59462). In addition to the viral particles, you will also receive purified pAAV-CAG-tdTomato (codon diversified) plasmid DNA. CAG-driven tdTomato expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    tdTomato (codon diversified)
    Available Since
    Oct. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. pJL-TRBO-G

    Plasmid
    #80083
    Purpose
    An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP.
    Depositor
    John Lindbo
    Article
    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.
    Insert
    GFP
    Expression
    Plant
    Promoter
    CaMV35S
    Available Since
    Nov. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    80083-plasmid-map-sequence-id-372997

    Enlarge

  8. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (AAV9)

    Viral Prep
    #105543-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (#105543). In addition to the viral particles, you will also receive purified pENN.AAV.cTNT.PI.eGFP.WPRE.rBG plasmid DNA. cTNT-driven eGFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    cTNT
    Tags
    EGFP
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only

  10. pCBC-MT1T2

    Plasmid
    #50593
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50593-plasmid-map-sequence-id-451910

    Enlarge

  12. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid
    #103870
    Purpose
    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    103870-plasmid-map-sequence-id-199178

    Enlarge

  13. sAJM.1505

    Bacterial Strain
    #108253
    Purpose
    Marionette-Pro. Marionette cluster inserted in the direction of leading strand replication, between 3,720,027 and 3,721,644 in E. coli BL21
    Depositor
    Christopher Voigt
    Article
    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038
    Bacterial Resistance
    Chloramphenicol
    Available Since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  19. pAAV-hSynapsin1-FLEx-axon-GCaMP6s (AAV9)

    Viral Prep
    #112010-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-FLEx-axon-GCaMP6s (#112010). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-FLEx-axon-GCaMP6s plasmid DNA. Synapsin-driven, cre-dependent axon-targeted GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.
    Promoter
    Syn
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  20. pLIX-hN1ICD

    Plasmid
    #91897
    Purpose
    Doxycycline inducible expression of human Notch1 ICD
    Depositor
    Julien Sage
    Insert
    Notch1 intracellular domain (NOTCH1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    codons 1770 to 2555 of human NOTCH1
    Promoter
    TRE promoter, Tet ON
    Available Since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91897-plasmid-map-sequence-id-180657

    Enlarge

  22. pADH99

    Plasmid
    #90979
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    US_HIS1
    FRT
    pENO1
    C. albicans Cas9
    pMAL2
    FLP
    NAT 1 of 2
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90979-plasmid-map-sequence-id-180505

    Enlarge

  27. pGP-AAV-syn-jGCaMP8m-WPRE (AAV5)

    Viral Prep
    #162375-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only

  34. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

    Enlarge

  37. pADH110

    Plasmid
    #90982
    Purpose
    NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    NAT 2 of 2
    pSNR52
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90982-plasmid-map-sequence-id-180510

    Enlarge

  40. AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)

    Plasmid
    #197509
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    ABE8e mutations in TadA and nSpCas9(D10A)
    Promoter
    Cbh
    Available Since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197509-plasmid-map-sequence-id-391242

    Enlarge

  47. pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (AAV1)

    Viral Prep
    #104493-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (#104493). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7b-WPRE plasmid DNA. Synapsin-driven, Cre-dependent jGCaMP7b expression. jGCaMP7b exhibits the brightest resting fluorescence and can be used for imaging of small neuronal processes (dendrites and axons). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  50. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid
    #218191
    Purpose
    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)
    Depositor
    Florence Pojer
    Article
    Rieubon et al protocols.io 2025
    Insert
    nucA
    Tags
    NSP4 signal secretion peptide and myc
    Expression
    Bacterial
    Mutation
    codon optimized ecoli residues - 22-266
    Promoter
    T7
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218191-plasmid-map-sequence-id-433865

    Enlarge

Showing: 7601 - 7650 of 171784 results