Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

313,479 results

Showing: 10301 - 10350 of 313479 results

  2. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172318
    Purpose
    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172318-plasmid-map-sequence-id-343646

    Enlarge

  3. R777-E139 Hs.NF1

    Plasmid
    #70423
    Purpose
    Gateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NF1 (NF1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70423-plasmid-map-sequence-id-125872

    Enlarge

  4. RedTrackCMV

    Plasmid
    #50957
    Purpose
    A backbone for adenovirus creation by the AdEasy method, similar to AdTrackCMV (Addgene Plasmid # 16405), except it co-expressed mRFP instead of eGFP
    Depositor
    James Bamburg
    Type
    Empty backbone
    Use
    Adenoviral
    Promoter
    CMV
    Available Since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    50957-plasmid-map-sequence-id-76710

    Enlarge

  6. AAV.TBG.PI.Cre.rBG

    Plasmid
    #107787
    Purpose
    AAV vector expressing Cre from TBG promoter
    Depositor
    James M. Wilson
    Has Service
    AAV8
    Insert
    Cre
    Use
    AAV
    Expression
    Mammalian
    Promoter
    TBG
    Available Since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    107787-plasmid-map-sequence-id-259375

    Enlarge

  13. VHL-NanoLuc Vector

    Plasmid
    #238827
    Purpose
    Express VHL-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    VHL (VHL Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238827-plasmid-map-sequence-id-481548

    Enlarge

  22. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV1)

    Viral Prep
    #105545-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    HI
    Tags
    EGFP
    Available Since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  29. pBAD24-sfGFPx2

    Plasmid
    #51559
    Purpose
    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)
    Depositor
    Sankar Adhya, Francisco Malagon
    Article
    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261
    Insert
    superfolder GFP
    Tags
    GLESTCRHASLAVLADERRFSA and MARARA
    Expression
    Bacterial
    Mutation
    Contains additonal aa in the N and C terminus (in…
    Available Since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    51559-plasmid-map-sequence-id-78831

    Enlarge

  31. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (AAV1)

    Viral Prep
    #125712-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE (#125712). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE plasmid DNA. CaMKIIa-driven expression of the optimized mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)
    Promoter
    short version of αCamKinase II promoter (0.4kb)
    Tags
    mScarlet
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  32. pLV-EF1A-CD19.8H.28TM.28.BB.3z

    Plasmid
    #200676
    Purpose
    Modular CD8hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Article
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD8HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Promoter
    EF1a-short
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200676-plasmid-map-sequence-id-391908

    Enlarge

  40. pBabe-Puro-BRAF-V600E

    Plasmid
    #15269
    Purpose
    Retroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)
    Depositor
    William Hahn
    Article
    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.
    Insert
    BRAF (BRAF Human)
    Use
    Retroviral
    Tags
    His and Myc
    Expression
    Mammalian
    Mutation
    Valine 600 to Glutamate (mutation has been found …
    Available Since
    July 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    15269-plasmid-map-sequence-id-217979

    Enlarge

  42. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

    Plasmid
    #52093
    Purpose
    Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine
    Depositor
    Dario Vignali
    Article
    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:
    Inserts
    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    Use
    Retroviral
    Tags
    F2A, IRES-EGFP, P2A, and T2A
    Expression
    Mammalian
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52093-plasmid-map-sequence-id-80507

    Enlarge

  44. pTREtight2

    Plasmid
    #19407
    Depositor
    Markus Ralser
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Oct. 15, 2008
    Availability
    Academic Institutions and Nonprofits only
    19407-plasmid-map-sequence-id-181154

    Enlarge

  45. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177433
    Purpose
    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.
    Depositor
    David Andrews
    Insert
    ActA tail anchor (actA Listeria monocytogenes)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177433-plasmid-map-sequence-id-348464

    Enlarge

Showing: 10301 - 10350 of 313479 results