Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

Applied Filters

20+ requests Clear filter

39,024 results

Showing: 1001 - 1050 of 39024 results

  9. pNPTS138 Cm

    Plasmid
    #41891
    Depositor
    Howard Steinman
    Type
    Empty backbone
    Use
    Bacterial allelic exchange vector with sacb
    Expression
    Bacterial
    Available Since
    Jan. 25, 2013
    Availability
    Academic Institutions and Nonprofits only
    41891-plasmid-map-sequence-id-365602

    Enlarge

  16. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid
    #73497
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
    Depositor
    Bruce Conklin
    Article
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Inserts
    dCas9-KRAB-P2A-mCherry
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available Since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73497-plasmid-map-sequence-id-247304

    Enlarge

  22. TFORF2069

    Plasmid
    #141902
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYC (MYC Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141902-plasmid-map-sequence-id-389798

    Enlarge

  23. EF1a_mCherry_P2A_Hygro

    Plasmid
    #135003
    Purpose
    Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    135003-plasmid-map-sequence-id-272070

    Enlarge

  24. pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (AAV8)

    Viral Prep
    #137163-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChR2-EYFP (#137163). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChR2-EYFP plasmid DNA. nEF-driven, Cre-dependent expression of ChR2-EYFP for optogenetic activation (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Cre-dependent)
    Available Since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only

  26. pLVX-FLAG-mCherry-C1-centrin-1

    Plasmid
    #73333
    Purpose
    Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags
    Depositor
    Manuel Thery
    Insert
    centrin-1 (CETN1 Human)
    Use
    Lentiviral
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73333-plasmid-map-sequence-id-139042

    Enlarge

  28. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Promoter
    Tight TRE promoter
    Available Since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-473508

    Enlarge

  32. Lenti-iCas9-neo

    Plasmid
    #85400
    Purpose
    Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence
    Depositor
    Qin Yan
    Article
    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14
    Insert
    Flag-iCas9-P2A-GFP
    Use
    Lentiviral
    Tags
    Flag-iCas9-P2A-GFP
    Available Since
    Jan. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    85400-plasmid-map-sequence-id-429030

    Enlarge

  35. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid
    #101893
    Purpose
    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons
    Depositor
    Ghassan Mouneimne
    Insert
    Lifeact
    Use
    Lentiviral
    Tags
    mTagBFP2
    Promoter
    CMV immediate early
    Available Since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    101893-plasmid-map-sequence-id-211159

    Enlarge

  38. pUC57-TPNOX

    Plasmid
    #87853
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    TPNOX
    Tags
    C-terminal FLAG tag with a linker
    Expression
    Bacterial
    Available Since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    87853-plasmid-map-sequence-id-175126

    Enlarge

  42. pLenti CMV rtTA3 Blast (w756-1)

    Plasmid
    #26429
    Purpose
    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast
    Depositor
    Eric Campeau
    Insert
    Tetracycline repressor A3 mutant
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    26429-plasmid-map-sequence-id-354259

    Enlarge

Showing: 1001 - 1050 of 39024 results