20,460 results

1. eBFP2 (eBFP2)_CD

   Plasmid

   #66034

   Purpose

   MoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Fluorescent reporter - BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR GFP-CaaX

   Plasmid

   #113020

   Purpose

   for lentivirus production of GFP-CaaX

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   GFP-CaaX
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET15bpATnp

   Plasmid

   #121137

   Purpose

   This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

   Depositor

   Keji Zhao

   Article

   Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

   Insert

   Protein A-Tnp
   

   Use

   Tags

   his

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR221_SLC1A5

   Plasmid

   #131974

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC1A5 (SLC1A5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. mCherry-BP1-2 pLPC-Puro

   Plasmid

   #19835

   Depositor

   Titia de Lange

   Article

   Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

   Insert

   Fragment of p53-Binding Protein 1 (TP53BP1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mutation

   Fragment of human 53BP1 aa 1220 - 1711

   Promoter

   Available since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDIRECT_25H

   Plasmid

   #91145

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Neurofascin-186-HA

   Plasmid

   #31061

   Depositor

   Vann Bennett

   Article

   Zhang et al J Biol Chem. 1998 Nov 13. 273(46):30785-

   Insert

   Neurofascin (Nfasc Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pACYC-FLAG-dN6-His

   Plasmid

   #22143

   Depositor

   Robert Sauer

   Article

   Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

   Insert

   Flag-clpXdeltaNLinkedHexamer-His6
   

   Use

   Tags

   Flag, His6, and L20 Linkers

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAB46 (CK2beta)

   Plasmid

   #27085

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2beta (CSNK2B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-Alpha-5-Integrin-12

    Plasmid

    #54970

    Purpose

    Localization: Focal Adhesions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Alpha-5 Integrin (ITGA5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Flag-Vac 14 Full Length

    Plasmid

    #47418

    Depositor

    Peter McPherson

    Article

    Lemaire et al FEBS Lett. 2006 Dec 22;580(30):6948-54.

    Insert

    Vac14 (VAC14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pGEX-GST-His6-2_HBD

    Plasmid

    #171870

    Purpose

    Expresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cells

    Depositor

    Kaiwei Liang

    Article

    Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

    Insert

    Tandem repeats Hybrid binding domain of Rnase H1
    

    Use

    Tags

    GST- and His10-tag

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. XE251 pCDNA3.1(zeo)-hDsh3

    Plasmid

    #16758

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-hDsh3 (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCherry-ATG5-C-18

    Plasmid

    #54995

    Purpose

    Localization: Membrane Receptor, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    ATG5 (ATG5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

15. GFP-LKB1-KM

    Plasmid

    #21148

    Depositor

    Junying Yuan

    Article

    Karuman et al Mol Cell. 2001 Jun . 7(6):1307-19.

    Insert

    GFP-LKB1-KM (Stk11 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K78M

    Promoter

    Available since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pGEX-αTAT1[2-236]

    Plasmid

    #27101

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    αTubulin K40 acetyltransferase (ATAT1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hysn-flex-dsRed-shscramble

    Plasmid

    #71383

    Purpose

    Expresses scramble shRNA Cre-dependently by flex switch

    Depositor

    William Wisden

    Article

    Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

    Insert

    human synapsin promoter-flex switch-dsRed-shscramble
    

    Use

    AAV, Cre/Lox, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV SYN flex PSAM4 GlyR IRES EGFP

    Plasmid

    #119741

    Purpose

    Cre-dependent chemogenetic inhibitor expression

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5 and AAV9

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    synapsin

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK7-FLAG-TSC1

    Plasmid

    #8995

    Depositor

    John Blenis

    Article

    Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

    Insert

    TSC1 (TSC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-GFPd2

    Plasmid

    #14760

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    EGFPd2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Human IgM-Fc

    Plasmid

    #158214

    Purpose

    Express the Fc region of human IgM

    Depositor

    Junyu Xiao

    Article

    Li et al Science. 2020 Feb 28;367(6481):1014-1017

    Insert

    IGHM (IGHM Human)
    

    Use

    Tags

    twin strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTD-NTwinStrep_Sm

    Plasmid

    #45937

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMX/cyclinB1 (17)

    Plasmid

    #26060

    Depositor

    Jonathon Pines

    Article

    Hagting et al Curr Biol. 1999 Jul 1. 9(13):680-9.

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

24. sAJM.1506

    Bacterial Strain

    #108254

    Purpose

    Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Bacterial Resistance

    Chloramphenicol

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

25. pCI neo-hEST2

    Plasmid

    #1781

    Depositor

    Bob Weinberg

    Article

    Meyerson et al Cell 1997 Aug 22;90(4):785-95.

    Insert

    hTERT (TERT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1_Floxed-STOP Fluc

    Plasmid

    #122962

    Purpose

    Firefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reaction

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_Floxed-STOP Fluc
    

    Use

    Cre/Lox and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHYRS52

    Plasmid

    #31122

    Depositor

    Hideo Iwai

    Article

    Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

    Insert

    Ulp1 (ULP1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Ulp1(403-621)

    Promoter

    Available since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-Neo-Bam p53 R249S

    Plasmid

    #16438

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R249S (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R249S

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGL2-AN

    Plasmid

    #20950

    Depositor

    William Kaelin

    Article

    Neuman et al Mol Cell Biol. 1994 Oct . 14(10):6607-15

    Insert

    E2F-1 promoter, genomic fragment (E2F1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    wt human E2F-1 promoter sequence

    Promoter

    Available since

    Aug. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Vinculin-venus

    Plasmid

    #27300

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Vinculin (VCL Chicken)
    

    Use

    Tags

    Venus A206K

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAVS1-Nst-CAG-DEST

    Plasmid

    #80489

    Purpose

    Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPP089

    Plasmid

    #158796

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-HA-NLGN4 WT

    Plasmid

    #59320

    Purpose

    Expression plasmid of HA-tagged wildtype human Neuroligin 4

    Depositor

    Peter Scheiffele

    Article

    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

    Insert

    NLGN4 (NLGN4X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA-flag-mZscan4d-polyA

    Plasmid

    #61831

    Purpose

    For mRNA synthesis or expression in mammalian cells

    Depositor

    Yi Zhang

    Article

    Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

    Insert

    Zscan4d (Zscan4d Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-cdk5GFP

    Plasmid

    #1346

    Depositor

    Li-Huei Tsai

    Insert

    CDK5 (CDK5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SpCas9-NG

    Plasmid

    #138566

    Purpose

    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEGFP rbFOX2

    Plasmid

    #63086

    Purpose

    Mammalian expression of EGFP tagged FOX2

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    RBFOX2 (RBFOX2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1-mASS1

    Plasmid

    #34576

    Depositor

    Peter Murray

    Insert

    ASS1 (Ass1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCE27

    Plasmid

    #174405

    Purpose

    C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. YAP1 (S6A) - V5 in pLX304

    Plasmid

    #42562

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S61A, S109A, S127A, S128A, S131A, S163A, S164A, S…

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. FR_HNF4A2

    Plasmid

    #31100

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713)

    Plasmid

    #60606

    Purpose

    Mammalian Display vector

    Depositor

    Wayne Marasco

    Article

    Taube et al PLoS One. 2008 Sep 11;3(9):e3181. doi: 1

    Insert

    PS11-scFvFc-CD28-gp41 (706-713) (CD28 Human)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

43. pmScarlet_H2A_C1

    Plasmid

    #85051

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    H2A (H2AC16 Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pHyg-AID*-GFP

    Plasmid

    #99521

    Purpose

    C-terminal AID*-GFP degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pTHSSe_50

    Plasmid

    #109248

    Purpose

    incW BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. Green monkey (Chlorocebus sabaeus) sgRNA library

    Pooled Library

    #178284

    Purpose

    Genome-wide CRISPR screening library targeting 20,991 genes in C. sabaeus, 84,963 total gRNAs

    Depositor

    John Doench, David Root

    Article

    Wei et al Cell. 2021 Jan 7;184(1):76-91.e13. doi:

    Expression

    Mammalian

    Species

    Chlorocebus sabaeus (african green monkey)

    Use

    CRISPR and Lentiviral

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

47. Flag-Gsdmd-NT 4A

    Plasmid

    #80952

    Purpose

    Expresses Gsdmd-NT 4A mutant in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

    Insert

    Gsdmd-NT 4A (Gsdmd Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE

    Plasmid

    #104491

    Purpose

    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP7s
    

    Use

    AAV and Cre/Lox

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMJ179

    Plasmid

    #85996

    Purpose

    One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    EGFP-NT2_cr2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified mouse U6

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. AgDD

    Plasmid

    #78289

    Purpose

    create protein aggregates in living cells

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NES-L106P-sfGFP (FKBP1A Human)
    

    Use

    Tags

    MLALKLAGLDI

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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