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164,349 results

Showing: 1501 - 1550 of 164349 results

  5. LifeAct-mScarletI pDRF1-GW

    Plasmid
    #168085
    Purpose
    Expresses Lifeact-mScarletI to visualize actin in yeast
    Depositor
    Bas Teusink
    Insert
    LifeAct-mScarletI
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168085-plasmid-map-sequence-id-332095

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  18. P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

    Plasmid
    #239102
    Purpose
    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.
    Depositor
    Karl Wahlin
    Article
    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472
    Insert
    POU4F2 (POU4F2 Human)
    Use
    Donor plasmid for integration into the human geno…
    Tags
    p2A-tdTomato-p2A-Thy1.2
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous POU4F2
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239102-plasmid-map-sequence-id-474475

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  23. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG, I253A, H310A, H435A

    Plasmid
    #222853
    Purpose
    Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc lacking FcRn binding
    Depositor
    Andrew Kruse
    Article
    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038
    Insert
    AT118-L
    Use
    Tags
    IgG1_Fc
    Expression
    Mammalian
    Mutation
    L234A, L235A, P329G, H435A, I253A, H310A
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    222853-plasmid-map-sequence-id-445015

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  24. pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG, I253A, H310A, H435A

    Plasmid
    #222855
    Purpose
    Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc lacking FcRn binding
    Depositor
    Andrew Kruse
    Article
    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038
    Insert
    AT118-L
    Use
    Tags
    IgG1_Fc
    Expression
    Mammalian
    Mutation
    L234A, L235A, P329G, H435A, I253A, H310A
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    222855-plasmid-map-sequence-id-445010

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  31. qTAG-N-Blast-mNeon-ACTB

    Plasmid
    #207751
    Purpose
    Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207751-plasmid-map-sequence-id-416864

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  32. TFORF2370

    Plasmid
    #142056
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MBD4 (MBD4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142056-plasmid-map-sequence-id-395678

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  37. LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode

    Plasmid
    #170236
    Purpose
    Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    CHUK (CHUK Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mutation
    S176E, S180E
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170236-plasmid-map-sequence-id-334484

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  38. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

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  39. RCP83

    Plasmid
    #163466
    Purpose
    Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP
    Depositor
    Frank Albert
    Article
    Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1
    Inserts
    Promoter fragment (Sharon2012_1922)
    His3 minimal promoter
    yeGFP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    163466-plasmid-map-sequence-id-322582

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  44. pGP-AAV-syn-iGABASnFR2-WPRE (AAV1)

    Viral Prep
    #218874-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-iGABASnFR2-WPRE (#218874). In addition to the viral particles, you will also receive purified pGP-AAV-syn-iGABASnFR2-WPRE plasmid DNA. Syn-driven expression of the improved GABA sensor iGABASnFR2 (positive change in fluorescence). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Kolb et al bioRxiv 2025.03.25.644953
    Promoter
    Syn
    Tags
    None
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  48. pAAV-hSyn-Cre-P2A-dTomato (AAV9)

    Viral Prep
    #107738-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Rylan Larsen
    Promoter
    Syn
    Tags
    dTomato (physically separate, not a fusion protein)
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  49. MSCV-opEBNA2-IRES-GFP

    Plasmid
    #220354
    Purpose
    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)
    Depositor
    Klaus Rajewsky
    Article
    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d
    Insert
    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    Use
    Retroviral
    Tags
    3 x HA tag
    Expression
    Mammalian
    Mutation
    codon-optimised for expression in mammalian cells
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    220354-plasmid-map-sequence-id-437068

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Showing: 1501 - 1550 of 164349 results