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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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171,694 results

Showing: 1751 - 1800 of 171694 results

  15. pMIG-w

    Plasmid
    #12282
    Purpose
    a bicistronic IRES-GFP containing vector which is a variant of pMIG that contains a posttranscriptional regulatory element from the woodchuck hepatitis virus
    Depositor
    Luk Parijs
    Article
    Refaeli et al J Exp Med. 2002 Oct 7. 196(7):999-1005.
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES GFP
    Expression
    Mammalian
    Available Since
    July 28, 2006
    Availability
    Academic Institutions and Nonprofits only

  16. CXCR7-Tango

    Plasmid
    #66265
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CXCR7 (ACKR3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  25. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

    Plasmid
    #181974
    Purpose
    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Neville Sanjana
    Article
    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:
    Insert
    FMC6.3-28z CAR; tNGFR (NGFR Synthetic, Human)
    Use
    Lentiviral
    Mutation
    NGFR truncation: aa 11-276
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    181974-plasmid-map-sequence-id-355578

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  27. yeast_DualReporter

    Plasmid
    #127546
    Purpose
    A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).
    Depositor
    Aviv Regev
    Article
    de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do
    Inserts
    bla
    mCherry
    URA3 (URA3 Budding Yeast)
    yEVenus
    Nat1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS
    Available Since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127546-plasmid-map-sequence-id-251803

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  31. Anti-PSD-95 [K28/43R] - Chimeric (Recombinant Antibody)

    Antibody
    #198594-rAb
    Purpose
    Anti-PSD-95 (Human) chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a chicken IgY Fc.
    Depositor
    James Trimmer and Melina Fan
    Article
    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1
    Recommended Applications
    Western Blot
    Reactivity
    Human
    Source Species
    Chicken
    Isotype
    IgY
    Trial Size
    Available to purchase
    Available Since
    April 3, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Addgene

  32. TFORF2973

    Plasmid
    #144449
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PPARD (PPARD Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144449-plasmid-map-sequence-id-407332

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  34. pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)

    Plasmid
    #127392
    Purpose
    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)
    Depositor
    Ryohei Yasuda
    Article
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    changed Threonine 286 to Aspartic acid and Threon…
    Promoter
    pCAG
    Available Since
    July 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    127392-plasmid-map-sequence-id-250433

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  36. GPR87-DuET

    Plasmid
    #213293
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR87 (GPR87 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213293-plasmid-map-sequence-id-430838

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  41. PA-mCherry1-C1

    Plasmid
    #54495
    Purpose
    Localization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    54495-plasmid-map-sequence-id-218682

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  47. TFORF0702

    Plasmid
    #144361
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NCOR2 (NCOR2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144361-plasmid-map-sequence-id-353414

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Showing: 1751 - 1800 of 171694 results