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Showing: 3001 - 3050 of 19980 results

  4. pAC-LYC

    Plasmid
    #53270
    Purpose
    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtE, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53270-plasmid-map-sequence-id-82990

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  7. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (AAV5)

    Viral Prep
    #35509-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (#35509). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only

  8. pLenti6.2-Nanoluc-ccdB

    Plasmid
    #87078
    Purpose
    Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87078-plasmid-map-sequence-id-173606

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  9. Marionette Sensor Collection

    Plasmid Kit
    #1000000137
    Purpose
    The Marionette Sensor Collection enables the modular control of up to 12 genes, by placing each one under the control of a small inducible promoter.
    Depositor
    Christopher Voigt
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only

  10. pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (AAV8)

    Viral Prep
    #137148-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (#137148). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of Arch3.3-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Cre and Flp-dependent)
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only

  11. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (AAV1)

    Viral Prep
    #100835-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (#100835). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project, Douglas Kim
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    CAG
    Tags
    None
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pCW57-RFP-P2A-MCS (Neo)

    Plasmid
    #89182
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89182-plasmid-map-sequence-id-176305

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  15. mKeima-Red-C1

    Plasmid
    #54546
    Purpose
    Localization: C1 Cloning Vector, Excitation: 440, Emission: 620
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mKeima-Red
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    54546-plasmid-map-sequence-id-256470

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  17. eSpCas9(1.1)

    Plasmid
    #71814
    Purpose
    Expresses high specificity SpCas9. Px330-like plasmid.
    Depositor
    Feng Zhang
    Article
    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.
    Has Service
    Cloning Grade DNA
    Insert
    enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71814-plasmid-map-sequence-id-177023

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  18. pET30a-HS-Tn5

    Plasmid
    #127916
    Purpose
    Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.
    Depositor
    Silin Zhong
    Article
    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:
    Insert
    Hyperstable Tn5
    Use
    Tags
    E coli elongation factor Ts and Mxe intein - Chit…
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    T7
    Available since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127916-plasmid-map-sequence-id-248754

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  20. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

    Plasmid
    #61628
    Purpose
    Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter
    Depositor
    Dolf Weijers
    Article
    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:
    Inserts
    ntdTomato
    n2xEGFP
    Use
    Tags
    Expression
    Plant
    Mutation
    2 copies of EGFP with N-terminal SV40 NLS
    Promoter
    Auxin-dependent DR5 promoter containing 9 reverse…
    Available since
    March 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    61628-plasmid-map-sequence-id-396550

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  22. MLM3636

    Plasmid
    #43860
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter
    Depositor
    Keith Joung
    Insert
    None
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43860-plasmid-map-sequence-id-194368

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  28. pAAV-mDlx-GFP-Fishell-1 (AAV9)

    Viral Prep
    #83900-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA. mDlx-driven expression of GFP in GABA-ergic interneurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    mDlx
    Tags
    GFP
    Available since
    Nov. 13, 2020
    Availability
    Academic Institutions and Nonprofits only

  29. ATG-2460

    Plasmid
    #108713
    Purpose
    EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)
    Depositor
    Keith Wood
    Article
    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10
    Insert
    CBR2opt-copGFP fusion
    Use
    Lentiviral
    Tags
    CBR2opt fused to copGFP
    Expression
    Mutation
    Promega used directed evolution to create CBR (Cl…
    Promoter
    elongation factor-1
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108713-plasmid-map-sequence-id-224540

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  31. pCDNA3 HA C9ORF72 op

    Plasmid
    #74156
    Purpose
    Express HA tagged optimized C9ORF72 in mammalian cells
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    C9ORF72 (C9orf72 Human, Synthetic)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    optimized sequence
    Promoter
    Available since
    April 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74156-plasmid-map-sequence-id-139165

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  32. rBiFC Kit

    Plasmid Kit
    #1000000147
    Depositor
    Christopher Grefen
    Available since
    March 11, 2019
    Availability
    Academic Institutions and Nonprofits only

  37. CMV-R-GECO1

    Plasmid
    #32444
    Purpose
    Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.
    Insert
    R-GECO1.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Substitutions relative to the mApple-derived anal…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    32444-plasmid-map-sequence-id-117093

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  40. pAAV-Ef1a-fDIO-JRGECO1a

    Plasmid
    #128317
    Purpose
    Expresses JRGECO1a under control of eukaryotic Ef1a promoter after Flp-dependent recombination.
    Depositor
    Patricia Jensen
    Has Service
    AAV1
    Insert
    NES-JRGECO1a
    Use
    AAV
    Tags
    His Tag
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128317-plasmid-map-sequence-id-257202

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  41. hSyn1-eYFP (AAV Retrograde)

    Viral Prep
    #117382-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from hSyn1-eYFP (#117382). In addition to the viral particles, you will also receive purified hSyn1-eYFP plasmid DNA. hSyn-driven eYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Article
    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:
    Promoter
    Syn
    Tags
    eYFP
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  42. pEGFP GR

    Plasmid
    #47504
    Purpose
    Expresses glucocorticoid receptor in mammalian cells
    Depositor
    Alice Wong
    Insert
    Glucocorticoid receptor (NR3C1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    47504-plasmid-map-sequence-id-223484

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  43. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid
    #124536
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1
    Inserts
    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    124536-plasmid-map-sequence-id-272178

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  44. pDIRECT_22C

    Plasmid
    #91135
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91135-plasmid-map-sequence-id-182261

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  48. pLSLGFP.R

    Plasmid
    #51501
    Purpose
    Single component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the replicon
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Insert
    GFP
    Use
    Plant t-dna plasmid
    Tags
    Expression
    Mutation
    Promoter
    2x35S
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51501-plasmid-map-sequence-id-261239

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  50. ZsGreen1-N1

    Plasmid
    #54702
    Purpose
    Localization: N1 Cloning Vector, Excitation: 493, Emission: 505
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    ZsGreen1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54702-plasmid-map-sequence-id-266653

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Showing: 3001 - 3050 of 19980 results