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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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178,032 results

Showing: 3401 - 3450 of 178032 results

  16. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140095-plasmid-map-sequence-id-292013

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  20. pAAV-hSyn-hChR2(H134R)-mCherry (AAV5)

    Viral Prep
    #26976-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-hChR2(H134R)-mCherry (#26976). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-mCherry plasmid DNA. Synapsin-driven, humanized channelrhodopsin H134R mutant, fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only

  22. pAAV.hSyn.iGluSnFr.WPRE.SV40 (AAV9)

    Viral Prep
    #98929-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.hSyn.iGluSnFr.WPRE.SV40 (#98929). In addition to the viral particles, you will also receive purified pAAV.hSyn.iGluSnFr.WPRE.SV40 plasmid DNA. Synapsin-driven, iGluSnFr glutamate sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Promoter
    Syn
    Available Since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only

  23. pAAV-hsyn-Jaws-KGC-GFP-ER2 (AAV5)

    Viral Prep
    #65014-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hsyn-Jaws-KGC-GFP-ER2 (#65014). In addition to the viral particles, you will also receive purified pAAV-hsyn-Jaws-KGC-GFP-ER2 plasmid DNA. hSyn-driven Jaws-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Promoter
    Syn
    Tags
    GFP
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  28. GLP1R-Tango

    Plasmid
    #66295
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GLP1R (GLP1R Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66295-plasmid-map-sequence-id-115875

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  29. MSP469

    Plasmid
    #65771
    Purpose
    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65771-plasmid-map-sequence-id-112653

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  38. pGFP(C3)-Vinculin

    Plasmid
    #30312
    Depositor
    Klaus Hahn
    Insert
    Vinculin (Vcl Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    F633S, V828E, L936P in mouse viniculin (NM_009502…
    Available Since
    July 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    30312-plasmid-map-sequence-id-116004

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  41. BcatMutS33/S37.T41/S45

    Plasmid
    #24204
    Depositor
    David Rimm
    Insert
    beta catenin (CTNNB1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    S33A, S37A, T41A,S45A
    Promoter
    CMV
    Available Since
    March 9, 2010
    Availability
    Academic Institutions and Nonprofits only
    24204-plasmid-map-sequence-id-235877

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  49. hTLR3 flag

    Plasmid
    #13084
    Depositor
    Ruslan Medzhitov
    Insert
    TLR3 (TLR3 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-17 have been replaced by mammalian …
    Available Since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13084-plasmid-map-sequence-id-229543

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Showing: 3401 - 3450 of 178032 results