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165,872 results

Showing: 4551 - 4600 of 165872 results

  3. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid
    #26976
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has Service
    AAV5, AAV8, and AAV9
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    hSyn
    Available Since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26976-plasmid-map-sequence-id-313556

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  7. pNUTN6His Y426F/Y517F hTFNG

    Plasmid
    #70084
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobe
    Depositor
    Anne Mason, Ross MacGillivray
    Article
    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2
    Insert
    mutated human serum transferrin (TF Human)
    Use
    Tags
    Hexa His tag and N-terminal signal peptide, 4 aa …
    Expression
    Mammalian
    Mutation
    Asn413 Asp, Asn611Asp, Ty426Phe, Tyr517Phe
    Promoter
    SV40
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70084-plasmid-map-sequence-id-129389

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  8. pNUT N6His Y95F/Y188F hTFNG

    Plasmid
    #69601
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe
    Depositor
    Anne Mason, Ross MacGillivray
    Article
    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2
    Insert
    mutated human serum transferrin (TF Human)
    Use
    Tags
    Hexa His tag and N-terminal signal peptide, 4 aa …
    Expression
    Mammalian
    Mutation
    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe
    Promoter
    SV40
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    69601-plasmid-map-sequence-id-124729

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  11. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226429-plasmid-map-sequence-id-458099

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  20. LT3GEPIR-CDK1shRNA1

    Plasmid
    #220569
    Purpose
    To inducibly knockdown CDK1 expression
    Depositor
    Sean Lee
    Insert
    CDK1 shRNA1 (CDK1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220569-plasmid-map-sequence-id-437943

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  21. GRM4-DuET

    Plasmid
    #213302
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM4 (GRM4 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213302-plasmid-map-sequence-id-431810

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  26. GPNMBA-c200

    Plasmid
    #205111
    Purpose
    GPNMB protein expression
    Depositor
    Paul Brennan
    Insert
    GPNMB (GPNMB Human)
    Use
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available Since
    Dec. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205111-plasmid-map-sequence-id-412364

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  39. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

    Plasmid
    #216154
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic exon and C-terminal FLAG
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216154-plasmid-map-sequence-id-437765

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  42. pAAV-CaMKIIa-eNpHR 3.0-EYFP (AAV9)

    Viral Prep
    #26971-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-eNpHR 3.0-EYFP (#26971). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-eNpHR 3.0-EYFP plasmid DNA. CaMKIIa-driven eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    EYFP
    Available Since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  45. lentiMPH v2 (Neo)

    Plasmid
    #92065
    Purpose
    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Insert
    MS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available Since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    92065-plasmid-map-sequence-id-181210

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Showing: 4551 - 4600 of 165872 results