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Mammalian Expression

76,239 results

Showing: 5351 - 5400 of 76239 results

  2. C3AR1-DuET

    Plasmid
    #213191
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    C3AR1 (C3AR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213191-plasmid-map-sequence-id-430756

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  5. pAK_DR30_CasRx_mCherry

    Plasmid
    #134844
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134844-plasmid-map-sequence-id-266704

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  7. tdTomato-VE-Cadherin-N-10

    Plasmid
    #58142
    Purpose
    Localization: Tight Junctions, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    VE-Cadherin (CDH5 Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    I503T in VE-Cadherin
    Promoter
    CMV
    Available since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. TFORF2538

    Plasmid
    #144036
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX4 (SOX4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    144036-plasmid-map-sequence-id-400532

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  13. GPR120-DuET

    Plasmid
    #213261
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR120 (FFAR4 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213261-plasmid-map-sequence-id-430811

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  22. pAAV-TetON-Bmpr1A-ca

    Plasmid
    #217536
    Purpose
    AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor
    Depositor
    Peter Scheiffele
    Article
    Okur et al bioRxiv 2023
    Insert
    constitutively active BMP receptor 1a (BMPR1A Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Tet operon (TetO)
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    217536-plasmid-map-sequence-id-429501

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  34. pAAV-CaMKII-ChrimsonR-tdTomato

    Plasmid
    #99231
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    CaMKII
    Available since
    Aug. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    99231-plasmid-map-sequence-id-219823

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  36. TFORF1218

    Plasmid
    #142157
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PRRX1 (PRRX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142157-plasmid-map-sequence-id-391449

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  46. TIRAP-GFP

    Plasmid
    #52739
    Purpose
    Retroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker
    Depositor
    Jonathan Kagan
    Article
    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10
    Insert
    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  50. CHRM5-Tango

    Plasmid
    #66252
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CHRM5 (CHRM5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 5351 - 5400 of 76239 results