Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

Your Selections

20+ requests

19,175 results

Showing: 5051 - 5100 of 19175 results

  2. pDEST-CMV-polysite

    Plasmid
    #122843
    Purpose
    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Type
    Empty backbone
    Use
    Gateway destination
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    122843-plasmid-map-sequence-id-244374

    Enlarge

  4. pLVTHM

    Plasmid
    #12247
    Purpose
    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid
    Depositor
    Didier Trono
    Article
    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12247-plasmid-map-sequence-id-352425

    Enlarge

  5. ZsGreen1-N1

    Plasmid
    #54702
    Purpose
    Localization: N1 Cloning Vector, Excitation: 493, Emission: 505
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    ZsGreen1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54702-plasmid-map-sequence-id-266653

    Enlarge

  8. pCDH-EF1-copGFP-T2A-Puro

    Plasmid
    #72263
    Purpose
    Express copGFP and puromycin resistance gene under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    elongation factor-1
    Available since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72263-plasmid-map-sequence-id-474982

    Enlarge

  9. pSLIK CA Rac1

    Plasmid
    #84605
    Purpose
    Lentiviral expression of doxycycline-inducible constitutively active Rac1 and co-expression of Venus
    Depositor
    Sanjay Kumar
    Article
    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94
    Insert
    Rac1 (RAC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Constitutively Active, Q61L
    Promoter
    Available since
    Nov. 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. pMSCVpuro-Mito-Pericam

    Plasmid
    #87381
    Purpose
    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
    Depositor
    Björn Stork
    Article
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    Mito-Pericam [ratiometric]
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87381-plasmid-map-sequence-id-212242

    Enlarge

  15. pBABE puro cyclinD1 HA

    Plasmid
    #9050
    Depositor
    William Hahn
    Insert
    cyclin D1 (CCND1 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only
    9050-plasmid-map-sequence-id-174149

    Enlarge

  16. p406TDH3

    Plasmid
    #15977
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    TDH3 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15977-plasmid-map-sequence-id-8462

    Enlarge

  20. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

    Enlarge

  24. psPAX2-D64V-NC-MS2

    Plasmid
    #122944
    Purpose
    MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles
    Depositor
    Baisong Lu
    Article
    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531
    Insert
    NC-MCP (MS2 coat protein) fusion protein in Gag
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D64V
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122944-plasmid-map-sequence-id-239322

    Enlarge

  44. pBOB-CAG-SARS-CoV2-Spike-HA

    Plasmid
    #141347
    Purpose
    3. Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao
    Depositor
    Gerald Pao
    Article
    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.
    Insert
    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    resynthesized with human codon optimization nucle…
    Promoter
    CAG
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    141347-plasmid-map-sequence-id-296391

    Enlarge

  46. pLenti puro HA-Ubiquitin

    Plasmid
    #74218
    Purpose
    Lentiviral expression of HA tagged Ubiquitin
    Depositor
    Melina Fan
    Insert
    ubiquitin (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Single ubiquitin coding sequence (full length UBB…
    Promoter
    CMV
    Available since
    March 14, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    74218-plasmid-map-sequence-id-148715

    Enlarge

Showing: 5051 - 5100 of 19175 results