165,343 results

1. pCBASceI

   Plasmid

   #26477

   Purpose

   I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site

   Depositor

   Maria Jasin

   Article

   Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

   Insert

   pCBASceI
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-TurboCas-NLS-Blast

   Plasmid

   #232346

   Purpose

   Lentiviral expression vector for dCas9 fused to miniTurboNLS

   Depositor

   Ali Shilatifard

   Article

   Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA, miniTurbo, V5 and 3xSV40 NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+HyPer7

   Plasmid

   #136466

   Purpose

   Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL1521 (pSPIN, pSC101* backbone)

   Plasmid

   #160729

   Purpose

   Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCF159_BRL

   Plasmid

   #225962

   Purpose

   CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-BRL (env protein)
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEcCas

   Plasmid

   #73227

   Purpose

   Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA

   Depositor

   Sheng Yang

   Article

   Li et al Acta Biochim Biophys Sin (Shanghai). 202

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR; Red recombinase

   Expression

   Bacterial

   Promoter

   native promoter

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEcgRNA

   Plasmid

   #166581

   Purpose

   CRISPR-Cas9-assisted genome editing in Escherichia coli

   Depositor

   Sheng Yang

   Article

   Li et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   ccdB
   

   Expression

   Bacterial

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tet-pLKO-puro

   Plasmid

   #21915

   Purpose

   3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

   Depositor

   Dmitri Wiederschain

   Article

   Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Aug. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. Grp1-PH pEGFP-C1

   Plasmid

   #71378

   Purpose

   For analysis of PH domain localization in mammalian cells

   Depositor

   Mark Lemmon

   Article

   Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

   Insert

   Grp1-PH (Cyth3 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Pleckstrin homology (PH) domain; aa 247-399

   Promoter

   CMV

   Available Since

   Nov. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA(MS2) cloning backbone

    Plasmid

    #61424

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral

    Plasmid

    #207507

    Purpose

    This plasmid can be used to generate retrovirus.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    tNGFR, HA-GD2-28z_CAR (NGFR Human)
    

    Use

    Retroviral

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-hSynI-V5-TurboID(N)-GPI

    Plasmid

    #163696

    Purpose

    Expresses n-terminal half of TurboID on surface of neurons

    Depositor

    Scott Soderling

    Article

    Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

    Insert

    GPI anchored TurboID
    

    Use

    AAV

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBiFC-VC155

    Plasmid

    #22011

    Purpose

    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    VC155
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Alanine 206 to Lysine

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-puro_GCaMP6s

    Plasmid

    #164589

    Purpose

    Stable integration and expression of GCaMP6s in mammalian cells

    Depositor

    Joseph Hyser

    Article

    Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

    Insert

    GCaMP6s
    

    Use

    Lentiviral

    Tags

    6xHis, T7 tag, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBiFC-VN173

    Plasmid

    #22010

    Purpose

    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    VN173
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Venus residus 1-172

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. ACS1

    Plasmid

    #13746

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    Acetyl CoA Synthetase 1 (Acss2 Mouse)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCasPA

    Plasmid

    #113347

    Purpose

    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5 alpha6

    Plasmid

    #16036

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    alpha6 (ITGA6 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFGET19_Ulp1

    Plasmid

    #64697

    Purpose

    H6-Ulp1 from pHYRS52 into pET-28b(+)

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Insert

    ULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Ulp1(403-621)

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCXLE-hOCT3/4-shp53-F

    Plasmid

    #27077

    Purpose

    Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    OCT3/4 (POU5F1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCXLE-hUL

    Plasmid

    #27080

    Purpose

    Integration-free (episomal) expression of human L-MYC and LIN28

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    L-MYC (LIN28A, MYCL Human)
    

    Expression

    Mammalian

    Available Since

    March 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLCNICK

    Plasmid

    #84653

    Purpose

    Genome editing for Lactobacillus casei Lc2W

    Depositor

    Sheng Yang

    Article

    Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

    Inserts

    Cas9 nickase
    sgRNA
    homology arms of LC2W_2179
    

    Use

    CRISPR

    Mutation

    D10A

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. VinculinTS

    Plasmid

    #26019

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Vinculin tension sensor
    

    Expression

    Mammalian

    Available Since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 4xHRE-MinTK-CRE-ODD

    Plasmid

    #141147

    Purpose

    lentiviral expression vector to generate a hypoxia fate-mapping system

    Depositor

    Daniele Gilkes

    Article

    Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

    Insert

    4xHRE-MinTK-CRE-ODD
    

    Use

    Lentiviral

    Mutation

    contains an altered Cre gene modified by the addi…

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pDGM6

    Plasmid

    #110660

    Purpose

    Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.

    Depositor

    David Russell

    Article

    Gregorevic et al Nat Med. 2004 Aug;10(8):828-34. doi: 10.

    Insert

    AAV6 cap genes, AAV2 rep genes, adenovirus helper genes
    

    Use

    AAV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCXLE-hSK

    Plasmid

    #27078

    Purpose

    Integration-free (episomal) expression of human SOX2 and KLF4

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    SOX2, KLF4 (KLF4, SOX2 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBAD-mTagBFP2

    Plasmid

    #34632

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    mTagBFP2
    

    Expression

    Bacterial

    Mutation

    I174A compared to mTagBFP (enhanced photostabilit…

    Available Since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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28. pACRISPR

    Plasmid

    #113348

    Purpose

    A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-EF1a-IRES-Puro

    Plasmid

    #85132

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CMV-loxp-DsRed-loxp-eGFP

    Plasmid

    #141148

    Purpose

    lentiviral expression vector to generate a hypoxia fate-mapping system

    Depositor

    Daniele Gilkes

    Article

    Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

    Insert

    loxp-DsRed-loxp-eGFP
    

    Use

    Lentiviral

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pU6-hROSA26-1-1kbdonor

    Plasmid

    #234736

    Purpose

    PseCAST crRNA targeting hROSA26-1, with 1 kb donor transposon

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    hROSA26-1 crRNA
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. lentiSAMv2

    Plasmid

    #75112

    Purpose

    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCS2FA CO Tol2 TPase

    Plasmid

    #133032

    Purpose

    Expression vector for production of codon optimized Tol2 transposase

    Depositor

    Charles Nathan Hancock

    Article

    Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

    Insert

    Codon optimized Tol2 Transposase
    

    Use

    In vitro expression

    Mutation

    Codon Optimized

    Available Since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. dCas9-VPR_P2A_mCherry

    Plasmid

    #154193

    Purpose

    Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Insert

    sp-dCas9-VPR_P2A_mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1as

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SpyCatcher003-sfGFP

    Plasmid

    #133449

    Purpose

    Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003-sfGFP
    

    Tags

    His6 and TEV site

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 4xmts-mScarlet-I

    Plasmid

    #98818

    Purpose

    In vivo visualization of mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    mts
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-PE7-P2A-GFP

    Plasmid

    #222996

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-EGFP
    

    Tags

    P2A-EGFP, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Ai9

    Plasmid

    #22799

    Purpose

    Rosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

    Insert

    CAG-Floxed tdTomato
    

    Use

    Mouse Targeting

    Available Since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLenti_PE7-2A-BSD

    Plasmid

    #234073

    Purpose

    For lentiviral expression of PE7 with blasticidin resistance

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Insert

    PE7
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF-1α core promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Plenti-CMV-MCS-RFP-SV-puro

    Plasmid

    #109377

    Purpose

    This plasmid expresses dsRed2 and puromycin resistance

    Depositor

    Behzad Gerami-Naini, Jonathan Garlick

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    dsRed2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge