165,965 results

1. mNeonGreen-KDEL

   Plasmid

   #226568

   Purpose

   Encodes mNeonGreen with KDEL ER lumenal target sequence

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   KDEL (ER lumenal target sequence)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_TetO_CD19-P2A-mCherry

   Plasmid

   #133803

   Purpose

   repressible expression of CD19 and mCherry in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   putative ECD of H. sapiens CD19 (CD19 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTreTight-Htt94Q-CFP

   Plasmid

   #23966

   Depositor

   Nico Dantuma

   Article

   Maynard et al Proc Natl Acad Sci U S A. 2009 Aug 18. 1

   Insert

   N-terminal fragment Huntingtin Q94 (HTT Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   94 polyQ repeat

   Available Since

   April 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-TetOne-Puro-MDM4

   Plasmid

   #195140

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of MDM4 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 (MDM4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ABE9

   Plasmid

   #194208

   Purpose

   Mammalian expression of ABE9

   Depositor

   Dali Li

   Article

   Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

   Insert

   TadA-8e (N108Q/L145T)-nSpCas9
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-mito-mTagBFP2

   Plasmid

   #216130

   Purpose

   Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOD_A0101

   Plasmid

   #90998

   Purpose

   Module A, Promoter: 35S, Gene: AtCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9
   

   Use

   CRISPR

   Promoter

   35S

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti NbALFA-mNeonGreen hygro

   Plasmid

   #193974

   Purpose

   lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, hygromycin resistance

   Depositor

   Raphael Gaudin

   Insert

   NbALFA-mNeonGreen
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPRa_all-in-one

   Plasmid

   #183695

   Purpose

   Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. rEstus

    Plasmid

    #209767

    Purpose

    To express a bright and sensitive genetically encoded voltage indicator in mammalian cells.

    Depositor

    Stefan Heinemann

    Article

    Rühl et al bioRxiv 2023.

    Insert

    rEstus
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNP1950

    Plasmid

    #222729

    Purpose

    J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. kiCAP-AAV-MyoAAV4A

    Plasmid

    #224448

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Mutation

    RGDYNSL insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUSE2ss-CLIg-mK_M18

    Plasmid

    #82358

    Purpose

    Expression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

    Insert

    mouse immunoglobulin kappa light chain, variable fragment derived from clone M18 (Igk, Igkc, Igk-V Mouse)
    

    Tags

    none

    Expression

    Mammalian

    Promoter

    hEF1-HTLV prom

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHIP_RNA2

    Plasmid

    #61260

    Purpose

    Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

    Depositor

    David Wang

    Article

    Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

    Insert

    Orsay RNA2
    

    Expression

    Worm

    Promoter

    heat shock promoter

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRGEB31

    Plasmid

    #51295

    Purpose

    Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.

    Depositor

    Yinong Yang

    Article

    Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Rice snoRNA U3 and dual 35S promoter

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T MDM2 WT

    Plasmid

    #16237

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. c-myc-PT3EF1a

    Plasmid

    #92046

    Purpose

    Expresses c-Myc in mammalian cells

    Depositor

    Xin Chen

    Article

    Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

    Insert

    C-MYC (MYC Human)
    

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    July 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pscALPSpuro-MmACE2 (Mouse)

    Plasmid

    #158087

    Purpose

    Expresses mouse ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Mouse ACE2 (Ace2 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PG13-luc (wt p53 binding sites)

    Plasmid

    #16442

    Purpose

    Luciferase reporter containing 13 copies of the p53-binding consensus sequence

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    p53-binding site (TP53 )
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRANS_221

    Plasmid

    #91115

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    35S

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. PiggyBac dCas9-KRAB

    Plasmid

    #191314

    Purpose

    piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    KRAB-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCSC-NGN2-IRES-GFP-T2A-Sox11

    Plasmid

    #90214

    Purpose

    To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

    Depositor

    Chun-Li Zhang

    Article

    Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

    Insert

    NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Mouse, Human)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDsRed-attP

    Plasmid

    #51019

    Purpose

    Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Inserts

    attP
    LoxP-3xP3-DsRed-LoxP
    

    Use

    High copy, amp resistance

    Promoter

    3xP3 and None

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. FLAG-FOXO3a WT

    Plasmid

    #8360

    Depositor

    Michael Greenberg

    Article

    Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGADT7-GW

    Plasmid

    #61702

    Purpose

    Gateway compatible Y2H vector - AD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAGGS-Stargazin-mEGFP-iLID

    Plasmid

    #178523

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p5E-elavl3

    Plasmid

    #75025

    Purpose

    Can be used to drive pan neuronal expression in zebrafish.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Promoter

    elavl3

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CaMKIIa-hM3D(Gq)-mCherry

    Plasmid

    #50476

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Has Service

    AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    CaMKIIa

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hAAVS1-gs4_EF1a-Cas9-2A-GFP

    Plasmid

    #118414

    Purpose

    CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    gRNA AAVS1 (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDH-CMV

    Plasmid

    #72265

    Purpose

    Express gene of interest under CMV promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pCK123 pDESTattB_exorh:mCerulean

    Plasmid

    #213512

    Purpose

    attB-containing pDEST plasmid, for use in Multisite Gateway reaction

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

    Plasmid

    #31482

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (Sce Target) EF Puro
    

    Use

    Lentiviral

    Mutation

    mCherry has M9S and M16L mutations. This is what …

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FUGW-d2GFP-ZEB1 3'UTR

    Plasmid

    #79601

    Purpose

    FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    d2GFP-Zeb1 3'UTR
    

    Use

    Lentiviral

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBA707

    Plasmid

    #186238

    Purpose

    KanR-BBR1 entry vector for HR donor

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEGFP Sec22b

    Plasmid

    #101918

    Purpose

    Expresses Sec22b in mammalian cells

    Depositor

    Thierry Galli

    Article

    Petkovic et al Nat Cell Biol. 2014 May;16(5):434-44. do

    Insert

    Sec22b (Sec22b Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CapChR2_pmScarlet-N1

    Plasmid

    #188032

    Purpose

    Expresses CapChR2-mScarlet in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2-mScarlet
    

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PB-CRX-T2A-NeuroD1

    Plasmid

    #194607

    Purpose

    Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

    Depositor

    Haruhisa Inoue

    Article

    Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

    Insert

    CRX-T2A-NeuroD1
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-DJ-N589X

    Plasmid

    #130878

    Purpose

    Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

    Depositor

    Prashant Mali

    Article

    Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

    Insert

    AAV-DJ cap N589X
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    N589X

    Promoter

    Unknown

    Available Since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDNA3.1-FLAG-hSF3B1-K700E

    Plasmid

    #82577

    Purpose

    Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

    Depositor

    Manoj Pillai

    Article

    Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

    Insert

    human SF3B1-K700E (SF3B1 Human)
    

    Tags

    FLAG peptide

    Expression

    Mammalian

    Mutation

    K700E

    Promoter

    CMV

    Available Since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mEGFP-8xbiRhoBAST

    Plasmid

    #196351

    Purpose

    Expression of mEGFP mRNA with 8xbiRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    mEGFP-8xbiRhoBAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAEMXT-eLACCO1.1

    Plasmid

    #167946

    Purpose

    Fluorescent biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    CD59-eLACCO1.1-GPI
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLminP_Luc2P_RE10

    Plasmid

    #90344

    Purpose

    IRF3 - Viral response gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    IRF3

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCS2+8CmCherry

    Plasmid

    #34935

    Depositor

    Amro Hamdoun

    Article

    Gokirmak et al J Biol Chem. 2012 Nov 2.

    Type

    Empty backbone

    Use

    Sea urchin, zebrafish, xenopus

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAV-hSyn-GFP-IRES-hSNCA

    Plasmid

    #185715

    Purpose

    AAV expression of GFP and human α-Synuclein from hSyn promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    synuclein, alpha (SNCA Human)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. ClpP-his

    Plasmid

    #22144

    Depositor

    Robert Sauer

    Article

    Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

    Insert

    ClpP-His6
    

    Tags

    his

    Expression

    Bacterial

    Mutation

    C-terminal His tag

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. dimericTomato-2xrGBD

    Plasmid

    #176098

    Purpose

    Visualization of active RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    rhotekin (Rtkn Mouse)
    

    Use

    Lentiviral

    Promoter

    CMVdel

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. U6>sgRNA(F+E)

    Plasmid

    #59986

    Purpose

    Empty vector for sgRNA cloning (F+E modified backbone)

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Type

    Empty backbone

    Use

    CRISPR

    Promoter

    Ciinte.U6

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. H52-pUCM-hNGN2-inv

    Plasmid

    #110492

    Purpose

    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

    Depositor

    Michael Ward

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    

    Use

    CRISPR, Cre/Lox, and TALEN

    Expression

    Mammalian

    Promoter

    CAG, EF-1 alpha, and TRE3G

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

    Plasmid

    #176836

    Purpose

    Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
    

    Use

    Unspecified

    Mutation

    See Depositor Comments Below

    Promoter

    Tet operator (tetracycline inducible expression)

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. GFP-TRIM28

    Plasmid

    #65397

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only