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159,117 results

Showing: 5501 - 5550 of 159117 results

  1. pPMS-1852

    Plasmid
    #232287
    Purpose
    Phage Display
    Depositor
    Jeremy Nathans
    Insert
    Fn3 domain #10
    Use
    Phage display vector
    Tags
    PreScission Cleavage, HA
    Expression
    Mutation
    D7K
    Promoter
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232287-plasmid-map-sequence-id-462128

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  2. pFN35K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid
    #229767
    Purpose
    Human Col4A3 tagged at N-terminal with SmBiT to be used with N-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system
    Depositor
    Hirofumi Kai
    Article
    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64
    Insert
    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    Use
    Luciferase
    Tags
    SmBiT
    Expression
    Mutation
    See Depositor Comments
    Promoter
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229767-plasmid-map-sequence-id-457423

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  4. pCJ1021

    Plasmid
    #228761
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Ift88 gRNAs (Ift88 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228761-plasmid-map-sequence-id-455784

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  9. pDONR223_HyPer7RgDAAO

    Plasmid
    #217746
    Purpose
    Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.
    Depositor
    Seth Parker
    Article
    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi
    Insert
    HyPer7 D amino acid oxidase
    Use
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Promoter
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217746-plasmid-map-sequence-id-442633

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  14. pLM433

    Plasmid
    #217293
    Purpose
    For creating CyanoTag vectors by Golden Gate cloning (BspQI version)
    Depositor
    Luke Mackinder
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    mNeonGreen - 3x FLAG
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217293-plasmid-map-sequence-id-435614

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  15. pCDB179-TfCut2-S121P-D174S-D204P

    Plasmid
    #218695
    Purpose
    Encodes codon optimized TfCut2-S121P-D174S-D204P enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    TfCut2
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    S121P-D174S-D204P
    Promoter
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218695-plasmid-map-sequence-id-434086

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  30. pLV-H1-SX

    Plasmid
    #174845
    Purpose
    empty vector for cloning shRNA and/or miRNA
    Depositor
    Helen Morrison
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174845-plasmid-map-sequence-id-344688

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  37. CXCR7-DuET

    Plasmid
    #213224
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CXCR7 (ACKR3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213224-plasmid-map-sequence-id-430786

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  38. pcDNA C12 ecDHFR DD YFP HA

    Plasmid
    #192815
    Purpose
    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.
    Depositor
    John Hulleman
    Article
    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021
    Insert
    E. coli dihydrofolate reductase
    Use
    Tags
    enhanced yellow fluorescent protein and hemagglut…
    Expression
    Mammalian
    Mutation
    W74R/T113S/E120D/Q146L
    Promoter
    CMV
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192815-plasmid-map-sequence-id-377240

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  43. BNLS

    Plasmid
    #50836
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP B
    Use
    Tags
    triplicated NLS sequence DPKKKRKV
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50836-plasmid-map-sequence-id-76280

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Showing: 5501 - 5550 of 159117 results