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8,883 results

Showing: 5701 - 5750 of 8883 results

  1. pCW57-GFP-P2A-MCS (Neo)

    Plasmid
    #89181
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89181-plasmid-map-sequence-id-176294

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  4. ELONGIN BC (XX01TCEB1A-c001)

    Plasmid
    #110274
    Purpose
    Elongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin C
    Depositor
    Nicola Burgess-Brown
    Insert
    Elongin BC (ELOC, ELOB Human)
    Expression
    Bacterial
    Mutation
    Elongin B is full length. Elongin C contains a.a…
    Promoter
    T7
    Available Since
    May 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    110274-plasmid-map-sequence-id-209817

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  8. pBa-MARK2-eGFP

    Plasmid
    #66706
    Purpose
    mammalian expression vector for MARK2 with a C-terminal eGFP
    Depositor
    Gary Banker, Marvin Bentley
    Insert
    MARK2 (MARK2 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    chicken beta actin
    Available Since
    June 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    66706-plasmid-map-sequence-id-114008

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  11. mCherry-Vinculin-23

    Plasmid
    #55159
    Purpose
    Localization: Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55159-plasmid-map-sequence-id-220874

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  13. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

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  15. pRK7

    Plasmid
    #10883
    Depositor
    John Blenis
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Feb. 13, 2006
    Availability
    Academic Institutions and Nonprofits only
    10883-plasmid-map-sequence-id-6809

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  20. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid
    #124536
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1
    Inserts
    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available Since
    June 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    124536-plasmid-map-sequence-id-272178

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  23. lentiCRISPR - EGFP sgRNA 2

    Plasmid
    #51761
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51761-plasmid-map-sequence-id-79377

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  25. PL-SIN-EOS-C(3+)-EiP

    Plasmid
    #21313
    Purpose
    Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance
    Depositor
    James Ellis
    Article
    Hotta et al Nat Methods. 2009 May . 6(5):370-6.
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EOS-C(3+) promoter region
    Available Since
    June 1, 2009
    Availability
    Academic Institutions and Nonprofits only
    21313-plasmid-map-sequence-id-9854

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  37. CXCR4-Tango

    Plasmid
    #66262
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CXCR4 (CXCR4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66262-plasmid-map-sequence-id-148151

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  41. pAD-LyseR

    Plasmid
    #99244
    Purpose
    For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)
    Depositor
    Jeff Hasty
    Article
    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102
    Insert
    phage lambda gene R (R phage lambda)
    Expression
    Bacterial
    Promoter
    beta-lactamase promoter
    Available Since
    Oct. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99244-plasmid-map-sequence-id-191038

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  45. pWPXLd

    Plasmid
    #12258
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12258-plasmid-map-sequence-id-7270

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  47. pMSCV-IRES-Blue FP

    Plasmid
    #52115
    Purpose
    a bicistronic IRES-Blue FP containing retroviral vector
    Depositor
    Dario Vignali
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES-Blue FP
    Expression
    Mammalian
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52115-plasmid-map-sequence-id-351562

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Showing: 5701 - 5750 of 8883 results