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19,771 results

Showing: 6101 - 6150 of 19771 results

  3. pCR2.1-InC

    Plasmid
    #83959
    Purpose
    Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.
    Depositor
    Kai-Shun Chen
    Insert
    Internal Control sequence
    Expression
    Bacterial
    Available Since
    Nov. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83959-plasmid-map-sequence-id-160468

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  4. pCAG-EGxxFP

    Plasmid
    #50716
    Purpose
    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.
    Depositor
    Masahito Ikawa
    Article
    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103
    Insert
    split EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    50716-plasmid-map-sequence-id-447281

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  12. pJ3 SHP1 C453S

    Plasmid
    #8573
    Depositor
    Ben Neel
    Insert
    SHP1 (PTPN6 Human)
    Expression
    Mammalian
    Mutation
    C453S (catalytically inactive)
    Available Since
    June 17, 2005
    Availability
    Academic Institutions and Nonprofits only

  14. mCherry-Lysosomes-20

    Plasmid
    #55073
    Purpose
    Localization: Lysosome Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    LAMP1
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55073-plasmid-map-sequence-id-215601

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  16. pTC-ApoE-Tet

    Plasmid
    #85578
    Purpose
    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liver
    Depositor
    Ursula Ehmer
    Article
    Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg
    Insert
    CreER
    Use
    Cre/Lox
    Expression
    Mammalian
    Promoter
    ApoE.HCR.hAAT
    Available Since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    85578-plasmid-map-sequence-id-169842

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  20. FLX1.8NFATGFPpd2HS4

    Plasmid
    #169096
    Purpose
    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation
    Depositor
    Geoffrey Owens
    Inserts
    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    Use
    Lentiviral
    Promoter
    NFAT enhancer + synthetic TATA box
    Available Since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169096-plasmid-map-sequence-id-338033

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  29. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191796
    Purpose
    vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized dCas13X
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    R84A, H89A, R739A, R740A, H745A, H746A, H747A
    Promoter
    CBh, SV40, hU6
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191796-plasmid-map-sequence-id-375578

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  34. pR26

    Plasmid
    #127372
    Purpose
    Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Article
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Promoter
    tight TRE promoter
    Available Since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127372-plasmid-map-sequence-id-247988

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  36. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-485302

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  41. Go1-CASE

    Plasmid
    #168123
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at L91/G92 within GNAO1
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168123-plasmid-map-sequence-id-332865

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  46. pSNAP-CaaX Control Plasmid

    Plasmid
    #101133
    Purpose
    Expression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Insert
    CaaX Box
    Tags
    SNAP-tag (SNAP26m)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101133-plasmid-map-sequence-id-194147

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Showing: 6101 - 6150 of 19771 results