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167,150 results

Showing: 481 - 520 of 167150 results

  8. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107943-plasmid-map-sequence-id-207614

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  17. pEF-BFP

    Plasmid
    #138272
    Purpose
    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555
    Insert
    BFP H66 PAM
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H66, PAM motif
    Promoter
    Ef1a
    Available Since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    138272-plasmid-map-sequence-id-273617

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  21. MSCV-IRES-GFP

    Plasmid
    #20672
    Purpose
    Depositor
    Tannishtha Reya
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES EGFP
    Expression
    Mammalian
    Available Since
    Aug. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20672-plasmid-map-sequence-id-203241

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  28. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid
    #104567
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator
    Depositor
    Keith Joung
    Article
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Insert
    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    Use
    CRISPR
    Tags
    NLS-3xHA-VPR
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available Since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104567-plasmid-map-sequence-id-201429

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  32. mEmerald-FilaminA-N-9

    Plasmid
    #54098
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    FilaminA (FLNA Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    D2626H
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54098-plasmid-map-sequence-id-491758

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  34. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid
    #73440
    Purpose
    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC-P3A2-vioD
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73440-plasmid-map-sequence-id-135339

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  37. pcDNA3-TfR-OVA

    Plasmid
    #64600
    Purpose
    Expression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentation
    Depositor
    Sandra Diebold, Martin Zenke
    Article
    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.
    Insert
    Ovalbumin
    Tags
    Transmembrane domain of Transferrin Receptor (TfR…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64600-plasmid-map-sequence-id-117969

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  39. pLenti CMV rtTA3 Hygro (w785-1)

    Plasmid
    #26730
    Purpose
    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 
    Depositor
    Eric Campeau
    Insert
    Tetracycline repressor A3 mutant
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    26730-plasmid-map-sequence-id-334693

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Showing: 481 - 520 of 167150 results