159,352 results

1. pQE60-EFTs-His

   Plasmid

   #124128

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Elongation factor Ts (tsf Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS2FA-H2B-mScarlet3

   Plasmid

   #224436

   Purpose

   Construct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   H2B-mScarlet3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV & SP6

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNF579

   Plasmid

   #26616

   Depositor

   Nancy Freitag

   Article

   Drevets et al Infect Immun. 2001 Mar . 69(3):1344-50.

   Insert

   gfpuv
   

   Use

   Tags

   gram-positive ribosome binding site

   Expression

   Mutation

   Promoter

   Available since

   Feb. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pg-HIF-1alpha-EGFP

   Plasmid

   #87204

   Purpose

   C-terminally EGFP-tagged human HIF-1a

   Depositor

   Violaine Sée

   Article

   Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

   Insert

   HIF-1a (HIF1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L40F, M88V, Q814R

   Promoter

   CMV

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS410

   Plasmid

   #11258

   Depositor

   Fred Cross

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMRX-IP-mGFP-TAX1BP1

   Plasmid

   #192803

   Purpose

   Stable expression of mGFP-TAX1BP1 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

   Insert

   NCOA4 (NCOA4 Human)
   

   Use

   Tags

   monomeric EGFP (A206K)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-15F11-HA-mCh

   Plasmid

   #129591

   Purpose

   The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ZIF-eGFP-HoTag6

   Plasmid

   #208745

   Purpose

   part B of SparkDrop

   Depositor

   Xiaokun Shu

   Article

   Chung et al ACS Cent Sci. 2023 Jul 7;9(7):1466-1479.

   Insert

   ZIF-eGFP-HoTag6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pATZ-Myc

   Plasmid

   #177718

   Purpose

   ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   alpha-1-antitrypsin (SERPINA1 Human)
   

   Use

   Tags

   Myc

   Expression

   Bacterial and Mammalian

   Mutation

   E342K (ATZ mutant)

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTALYM3B15

    Plasmid

    #47878

    Purpose

    TALE-mClover against mouse major satellite sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mClover
    

    Use

    TALEN

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

    Plasmid

    #61592

    Purpose

    A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Insert

    hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-hSyn-miniDq-P2A-mCherry

    Plasmid

    #204357

    Purpose

    AAV vector for miniDq expression under the control of human synapsin promoter

    Depositor

    Masao Doi

    Article

    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

    Insert

    miniDq-P2A-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    The third intracellular loop (ICL3) of hM3Dq was …

    Promoter

    hSyn

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEF6a-CD28-PafA

    Plasmid

    #113400

    Purpose

    Expresses CD28-PafA in mammalian cell

    Depositor

    Min Zhuang

    Article

    Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

    Insert

    CD28-PafA
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_SMAD5_WT

    Plasmid

    #82217

    Purpose

    Gateway Donor vector containing SMAD5 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SMAD5 (SMAD5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mT-Sapphire-N1

    Plasmid

    #54569

    Purpose

    Localization: N1 Cloning Vector, Excitation: 399, Emission: 511

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al Nat Methods. 2008 May . 5(5):401-3.

    Type

    Empty backbone

    Use

    Tags

    mT

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pSynSRE-T-Luc

    Plasmid

    #60444

    Purpose

    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

    Depositor

    Timothy Osborne

    Article

    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

    Insert

    HMG-CoA synthase promoter (-324/-225)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFORF1884

    Plasmid

    #143984

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZIC2 (ZIC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSC101-Donor

    Plasmid

    #140630

    Purpose

    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-MTS-jAspSnFR3-mRuby3

    Plasmid

    #203492

    Purpose

    Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    MTS-jAspSnFR3-mRuby3
    

    Use

    Lentiviral

    Tags

    His-tag, Mitochondrial localization signal, and m…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. U6-AsCas12f-full-length-sgRNA

    Plasmid

    #204638

    Purpose

    Expression of full-length AsCas12f sgRNA in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    full-length AsCas12f sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-hATG7wt

    Plasmid

    #87867

    Purpose

    Express a wild type human Atg7/Apg7 in mammalian cells

    Depositor

    Eiki Kominami, Isei Tanida

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    ATG7 (ATG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-FLAG-DDB1

    Plasmid

    #19918

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    DDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFB1_hMSH6

    Plasmid

    #129424

    Purpose

    Expression human MSH6 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH6 (MSH6 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. OpenCRISPR-1 sgRNA-16nt stem

    Plasmid

    #221566

    Purpose

    Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al bioRxiv 2024.04.22.590591

    Insert

    OpenCRISPR-1 sgRNA 16nt stem loop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. PRDX6A-c001

    Plasmid

    #175474

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    PRDX6 (PRDX6 Human)
    

    Use

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Mutation

    See Depositor Comments

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV_BiCHATe27_dTomato_nlsdTomato

    Plasmid

    #213829

    Purpose

    AAV construct expressing dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_dTomato_nlsdTomato

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB

    Insert

    dTomato_P2A_nls.dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHDM-Hgpm2

    Plasmid

    #164441

    Purpose

    Encodes codon-optimized HIV gagpol for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    Codon Optimized HIV-1 GagPol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TagBFP2-PIP4K2A

    Plasmid

    #202726

    Purpose

    expresses fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    mTagBFP2:PIP4K2A (PIP4K2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pscALPSpuro-MjACE2 (Pangolin)

    Plasmid

    #158084

    Purpose

    Expresses pangolin ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Pangolin ACE2 (ACE2 )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

    Plasmid

    #100834

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CamKII promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CamKII

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Flag-HA-OTUD4

    Plasmid

    #22594

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    OTUD4 (OTUD4 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET21b FAST-PETase

    Plasmid

    #225760

    Purpose

    Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    FAST-PETase
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET15b-His-RF2

    Plasmid

    #124130

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Release factor 2 (prfB Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PB-CMV-MCS-Puro-EGFP

    Plasmid

    #219795

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDV10_pCMV-ADAR1-p150

    Plasmid

    #200405

    Purpose

    Expresses ADAR1 (p150 isoform) constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    ADAR-p150
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCherry.90 beta

    Plasmid

    #108223

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    mCherry-Hsp90b (HSP90AB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Lyso-B

    Plasmid

    #209873

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGWB550

    Plasmid

    #74880

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-Flag-Tet1 CD Mut

    Plasmid

    #72218

    Purpose

    Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 1 (Tet1 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET1 catalytic domain (cata…

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

40. pLPO2

    Plasmid

    #209976

    Purpose

    Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    replication origin (SH71_rep)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyA

    Plasmid

    #172911

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Ace2N-4AA-mNeon A122D
    

    Use

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCAG-Lyn11-FRB

    Plasmid

    #155228

    Purpose

    membrane anchor for FKBP-FRB system

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Membrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pHAGE-RSV-tdTomato-2A-GCaMP6s

    Plasmid

    #80316

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Darrell Kotton

    Article

    Liberti WA et al Nat Neurosci. 2016 Oct 10. doi: 10.1038/

    Inserts

    tdTomato
    GCaMP6s
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    RSV

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pQE60-RRF-His

    Plasmid

    #124132

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Ribosome recycling factor (frr Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pWZL Hygro-NMYC TRF2 deltaB deltaM

    Plasmid

    #16355

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF2 (TERF2 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

46. pCMV-PV-NES-GFP

    Plasmid

    #17301

    Depositor

    Anton Bennett

    Article

    Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

    Insert

    PV-NES-GFP (Pvalb Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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47. pMSCV-IRES-CFP II (pMIC II)

    Plasmid

    #52109

    Purpose

    a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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48. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

    Plasmid

    #67980

    Purpose

    Cas9 activity reporter with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. SGEP

    Plasmid

    #111170

    Purpose

    miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    SFFV

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pYAL_HLA-A2_MART1

    Plasmid

    #171177

    Purpose

    Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

    Depositor

    Michael Birnbaum

    Article

    Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

    Insert

    MART1_HLA-A2*01 single-chain trimer fusion
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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