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177,170 results

Showing: 8201 - 8250 of 177170 results

  9. Dead-Laconic/pcDNA3.1(-)

    Plasmid
    #118627
    Purpose
    To express in mammalian cells a lactate FRET sensor negative control.
    Depositor
    Luis Felipe Barros
    Publication
    Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dead-Laconic
    Expression
    Mammalian
    Mutation
    Change Histidine 151 to Aspartic Acid of Escheric…
    Promoter
    CMV Promoter
    Available since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    118627-plasmid-map-sequence-id-231761

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  12. pC1-SypHer3s-IMS

    Plasmid
    #108120
    Purpose
    Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.
    Depositor
    Vsevolod Belousov
    Publication
    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SypHer3s-IMS
    Tags
    ratiometricYFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108120-plasmid-map-sequence-id-262557

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  13. AICSDP-42:AAVS1-mTagRFPT-CAAX

    Plasmid
    #107580
    Purpose
    Homology arms, promoter and linker-mTagRFPT-CAAX sequence for internal insertion of CAGGS-driven mTagRFPT-CAAX at the AAVS1 safe harbor location in human cells
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    May 9, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    5 Articles
    107580-plasmid-map-sequence-id-208159

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  15. pcDNA-NES-KIRIN1

    Plasmid
    #107416
    Purpose
    Mammalian cell expression plasmid for potassium ion ratiometric indicator KIRIN1 with nuclear exclusion sequence
    Depositor
    Robert Campbell
    Publication
    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIRIN1
    Tags
    Nuclear exclusion sequence (NES) (LALKLAGLDIGS)
    Expression
    Mammalian
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107416-plasmid-map-sequence-id-207270

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  16. pRPF215

    Plasmid
    #106377
    Purpose
    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon
    Depositor
    Robert Fagan, Neil Fairweather
    Publication
    Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1
    Insert
    Ptet-himar1-Ter(slpA)-transposon
    Use
    E. coli - c. difficile shuttle vector
    Mutation
    Codon optimised for C. difficile
    Promoter
    Ptet
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    106377-plasmid-map-sequence-id-205135

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  22. pBR322-TIMER

    Plasmid
    #103056
    Purpose
    medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)
    Depositor
    Dirk Bumann
    Publication
    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1
    Insert
    TIMER-bac
    Expression
    Bacterial
    Mutation
    mutations: S4T and S179T
    Promoter
    constitutive ybaJp promoter
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    103056-plasmid-map-sequence-id-199055

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  28. pJYS1Peftu

    Plasmid
    #85546
    Purpose
    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter
    Depositor
    Sheng Yang
    Publication
    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.
    Inserts
    Cpf1
    recT
    Use
    CRISPR; Shuttle vector corynebacterium glutamicum…
    Expression
    Bacterial
    Promoter
    etfu and unknown
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    85546-plasmid-map-sequence-id-176968

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  29. pL97

    Plasmid
    #78576
    Purpose
    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter
    Depositor
    Xuming Mao
    Publication
    Sun et al Annals of Microbiology Dec 2012, Volume
    Type
    Empty backbone
    Use
    Streptomyces expression
    Expression
    Bacterial
    Promoter
    ermEp* promoter from S. erythraea
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    78576-plasmid-map-sequence-id-421926

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  30. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    86612-plasmid-map-sequence-id-168849

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  34. mCyRFP1-C1

    Plasmid
    #84355
    Purpose
    Expresses mCyRFP1 fluorescent protein in an empty backbone
    Depositor
    Ryohei Yasuda
    Publication
    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCyRFP1
    Expression
    Mammalian
    Mutation
    A161K and N41A, reletive to CyRFP1
    Promoter
    CMV
    Available since
    Nov. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    84355-plasmid-map-sequence-id-160941

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  36. c3GIC9

    Plasmid
    #62191
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Publication
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. Pyogenes Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Promoter
    TRE3G
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    62191-plasmid-map-sequence-id-156341

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  37. pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

    Plasmid
    #74309
    Purpose
    Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells
    Depositor
    Ali Guler
    Publication
    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Human, Rat)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    74309-plasmid-map-sequence-id-153122

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  41. pEGFP-centrin-1

    Plasmid
    #72641
    Purpose
    The plasmid encodes centrin-1 with an N-terminal EGFP tag
    Depositor
    Michel Bornens
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    centrin-1 (CETN1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    72641-plasmid-map-sequence-id-132790

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  44. pCITF-KCC2

    Plasmid
    #61404
    Purpose
    Mammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequence
    Depositor
    Franck Polleux
    Publication
    Bortone et al Neuron. 2009 Apr 16;62(1):53-71. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KCC2 (SLC12A5 Human)
    Tags
    IRES-tdTomato-Flag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    61404-plasmid-map-sequence-id-122754

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  46. GFP-BRDT

    Plasmid
    #65381
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRDT (BRDT Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    65381-plasmid-map-sequence-id-245402

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  47. BiP-mCherry

    Plasmid
    #62233
    Purpose
    Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.
    Depositor
    Erik Snapp
    Publication
    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hamster BiP-mCherry-KDEL
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    62233-plasmid-map-sequence-id-314153

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  50. mCherry-MAPTau-C-10

    Plasmid
    #55077
    Purpose
    Localization: Microtubules, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPTau (MAPT Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55077-plasmid-map-sequence-id-353040

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Showing: 8201 - 8250 of 177170 results