162,408 results
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Plasmid#184463PurposepENN.AAV.TBG.PI.N-FLAG-mSTAT5bCA.WPRE.bGH express N-flag tag STAT5bCA carrying a point mutation at base 1963 (A to C), which changes Asn642 to His, conferring constitutive activity (CA) to STAT5b.DepositorInsertN-Flag-mSTAT5bCA (Stat5b Mouse)
UseAAVTagsFLAG TagExpressionMammalianMutationPoint mutation at base 1963 (A to C), which chang…PromoterAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBRPB CAG-mCherry-IP
Plasmid#106333PurposePiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.DepositorInsertmCherry-IRES-Puro
UsePiggybac transposonTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-Bag6ΔUBL
Plasmid#61837Purposeexpress N-terminally FLAG tagged human Bag6 without UBL domainDepositorInsertBag6 (BAG6 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_H2B_HaloTag7
Plasmid#169329PurposeExpression of H2B-HaloTag7 in mammalian cellsDepositorInsertH2B-HaloTag7 (H2BC21 Human)
UseTagsHaloTag7ExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Puro
Plasmid#227256PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5M-ADAM10
Plasmid#31717DepositorInsertADAM10 (ADAM10 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Tubb3-GFP KI
Plasmid#131497PurposeEndogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2547
Plasmid#144039PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCEBPD (CEBPD Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NR2B
Plasmid#17925DepositorInsertN-methyl D-aspartate receptor 2B (Grin2b Rat)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGF-GAS4X
Plasmid#225751PurposeThis plasmid was designed to selectively monitor the activation of the STAT1 signaling pathway in NK cells, a pathway known to play a crucial role in regulating NK cells' activation.DepositorInsertinterferon gamma activating sequence (GAS) (STAT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab27A
Plasmid#89237PurposeExpression of GFP-tagged human Rab27A in mammalian cellsDepositorInserthuman Rab27A (RAB7A Human)
UseTagseGFPExpressionMammalianMutationwild typePromoterCMVAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-NCAM1-Fc(DAPA)-AviTag-6xHis
Plasmid#156948PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertNCAM1 (NCAM1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE3g-mNeon-WPRE-pA
Plasmid#192064PurposemNeonGreen expression with TRE3g promoterDepositorInsertmNeonGreen
UseAAVTagsExpressionMammalianMutationPromoterTRE3gAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFCIV-CEACAM6
Plasmid#149303Purposelentiviral packaging vector for CEACAM6 expression.DepositorInsertCEACAM6 (CEACAM6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterubiquitin CAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab11a-7
Plasmid#56444PurposeLocalization: Ras GTPase, Excitation: 488, Emission: 507DepositorInsertRab11a (RAB11A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK1009 (pSL9)
Plasmid#191004PurposeExpresses E2 Crimson on a broad host range pBBR1 origin plasmidDepositorInsertE2-Crimson
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterCP25Available sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
MTS-mCherry-GFP1-10-Hyg-N1
Plasmid#91957PurposeExpresses mCherry-GFP1-10 in mammalian cell mitochondriaDepositorInsertMTS-mCherry-GFP1-10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_WT
Plasmid#83482PurposeBacterial expression of WT L. buccalis C2c2 CRISPR effector.DepositorInsertLbu_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF0208
Plasmid#142556PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXP2 (FOXP2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT3T4
Plasmid#50592PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT3, gRNA scaffold, U6-1tplus, U6-26p, T4
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-tomato SOX2-sgRNA
Plasmid#196190PurposeEditing human SOX2 locusDepositorInsertSOX2 (SOX2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-mRunx1c
Plasmid#64894PurposeLentiviral expression of Flag-tagged murine transcription factor Runx1cDepositorInsertRunx1c (Runx1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Tmem192-mRFP-3xHA
Plasmid#134631Purposebait for affinity-isolation of lysosomesDepositorInsertTMEM192 (TMEM192 Human)
UseLentiviralTags3xHA, TEV, and mRFPExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CAG∞RFP
Plasmid#154016PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposaseDepositorInsertmRFP1
UseTagsExpressionMammalianMutationH210PPromoterCAGAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAMD1-2
Plasmid#61564PurposeStandardized mini-Tn5 delivery plasmid, kanamycin resistantDepositorTypeEmpty backboneUseSynthetic Biology; Mini-tn5 vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
p70a-T7RNAP
Plasmid#105683PurposeExpresses T7 polymerase in cell-free TXTL.DepositorInsertT7 polymerase
UseTagsExpressionBacterialMutationPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
piRFP-N3-Tyrosinase
Plasmid#80152PurposeMelanosome markerDepositorInsertTyrosinase (TYR Human)
UseTagsiRFP682ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-mhYFP
Plasmid#186532PurposeMonomeric hyperfolder YFP fluorescent protein: bacterial expression plasmidDepositorInsertMonomeric hyperfolder YFP
UseTags6xHisExpressionBacterialMutationhfYFP-S147P/L195M/V206KPromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
ssFv_lenti_mutATMSPARK2
Plasmid#195250Purposeexpress Thr to Ala mutant ATMSPARK2 in a lenti vectorDepositorInsertThr to Ala mutant ATMSPARK2
UseLentiviralTagsExpressionMammalianMutationPromoterssfvAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-FURIN-G418
Plasmid#158462PurposeConstitutive lentiviral expression of human FURIN.DepositorInsertFURIN (FURIN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCX-OKS-2A
Plasmid#19771PurposeNon-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2DepositorUseTagsExpressionMammalianMutationThree genes are connected with the foot-and-mouth…PromoterAvailable sinceJan. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
UseTags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
14UAS PSD95:GFP 5UAS DSRedExpress
Plasmid#74315PurposeEncodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafishDepositorInsertszebrafish PSD95
DsRedExpress
UseTagsEGFPExpressionMutationY599H and I793S (please see depositor comment bel…PromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-bARGSer-AxeTxe
Plasmid#192473PurposeSecond-generation bacterial acoustic reporter gene derived from SerratiaDepositorInsertsbARGSer
AxeTxe
UseTagsExpressionBacterialMutationthe gene Ser39006_001280 was deleted from the clu…PromoterNative promoter from Enterococcus faecium and pBADAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL1-FLAG
Plasmid#15818DepositorInsertCullin1 (CUL1 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N452PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pE-35S-miniSdd7-nCas9-PBE-GmPPO2
Plasmid#204855PurposeFor cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)DepositorInsertminiSdd7-nSpCas9(D10A)-UGI-mScarlet
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9Promoter35S, AtU6-26pAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
IDP456
Plasmid#87002Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertbarcode landing site-mCherry-4xboxB
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH-MBP-CbAgo
Plasmid#127707PurposepET His6 MBP TEV CbAgoDepositorInsertCbAgo
UseTagsMPBExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPTN-EGFP
Plasmid#27052DepositorInsertoptineurin (OPTN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PD-L1
Plasmid#121492PurposeExpression of human PD-L1 WT in mammalian cellsDepositorInsertPD-L1 (CD274 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-IRES eGFP
Plasmid#128059PurposeLentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
gCH132 (crCD55-4_crB2M-1_crKIT-2_crKIT-3_crCD81-1)
Plasmid#217341PurposecrRNA array targeting CD81, B2M, KIT, CD55DepositorInsertsUseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tag5Amyc-GSK3b WT
Plasmid#16260DepositorInsertGSK3 beta (GSK3B Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (TBE 1/2 - wt)
Plasmid#16595DepositorInsertc-myc promoter (TBE 1/2 wt) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
gCH29 (crCD81-1)
Plasmid#217344PurposecrRNA targeting CD81DepositorInsertcrCD81-1 (CD81 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-B
Plasmid#104448PurposeExpression of human VAP-B fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein B (VAPB Human)
UseTagsEGFP and HAExpressionMammalianMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 mCherry
Plasmid#128073Purposepuro exchanged to mCherryDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationnoPromoterAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rluc8-NbV5
Plasmid#201479PurposeBRET detection of V5-tagged proteinDepositorInsertNbV5-Rluc8
UseTagsRluc8ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag
Plasmid#209665PurposeExpression of iATPSnFR2, low affinityDepositorInsertiATPSnFR2.A95A.A119L.HaloTag
UseAAVTagsExpressionMutationA95A.A119LPromoterhSynAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only