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159,284 results

Showing: 8301 - 8350 of 159284 results

  4. TFORF2652

    Plasmid
    #142969
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ARNTL2 (BMAL2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142969-plasmid-map-sequence-id-397337

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  7. PTGER2-Tango

    Plasmid
    #66484
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    PTGER2 (PTGER2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66484-plasmid-map-sequence-id-276276

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  9. pCMV-PE6f

    Plasmid
    #207856
    Purpose
    Mammalian expression of PE6f prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6f
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207856-plasmid-map-sequence-id-408168

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  10. CCR8-DuET

    Plasmid
    #213204
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR8 (CCR8 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213204-plasmid-map-sequence-id-430767

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  13. TFORF3398

    Plasmid
    #144874
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HLF (HLF Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144874-plasmid-map-sequence-id-404438

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  16. TFORF0210

    Plasmid
    #142557
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXP2 (FOXP2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142557-plasmid-map-sequence-id-395974

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  17. pSBtet-GP

    Plasmid
    #60495
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60495-plasmid-map-sequence-id-237935

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  18. h-Menkes

    Plasmid
    #196953
    Purpose
    Plasmid expressing an antigen targeting the Menke's disease copper ATPase
    Depositor
    James Trimmer
    Insert
    Copper ATPase 1 (ATP7A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    196953-plasmid-map-sequence-id-391766

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  19. GANLS-DEVD-BNES

    Plasmid
    #50835
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP A and ddRFP B
    Use
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50835-plasmid-map-sequence-id-76278

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  23. BiP-mGFP

    Plasmid
    #62231
    Purpose
    Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.
    Depositor
    Erik Snapp
    Article
    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2
    Insert
    hamster BiP-mGFP-KDEL
    Use
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    62231-plasmid-map-sequence-id-314151

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  26. pHLmMBP-10

    Plasmid
    #72348
    Purpose
    Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72348-plasmid-map-sequence-id-133757

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  29. pUC18-mini-Tn7T-Gm-LacZ10

    Plasmid
    #65026
    Purpose
    GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 1. See Depositor Comments below for information regarding the lacZ sequence.
    Depositor
    Herbert Schweizer
    Article
    Choi et al Nat Protoc. 2006;1(1):153-61.
    Type
    Empty backbone
    Use
    Tags
    lacZ
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    65026-plasmid-map-sequence-id-109990

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  32. PTGIR-Tango

    Plasmid
    #66488
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    PTGIR (PTGIR Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  34. pCAGGS-mTagBFP2-T2A-FLPo

    Plasmid
    #124834
    Purpose
    Monomeric TagBFP2 and FLP recombinase
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-FLPo
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    124834-plasmid-map-sequence-id-247146

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  36. pDONR221-SLC25A10_STOP

    Plasmid
    #161180
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC25A10 (SLC25A10 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161180-plasmid-map-sequence-id-334675

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  38. pDONR221-SLC25A42_STOP

    Plasmid
    #161184
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC25A42 (SLC25A42 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161184-plasmid-map-sequence-id-352200

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  40. pAC-BETAipi

    Plasmid
    #53277
    Purpose
    Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.
    Insert
    idi
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoter
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53277-plasmid-map-sequence-id-83121

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  47. TFORF1457

    Plasmid
    #143501
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TFEB (TFEB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143501-plasmid-map-sequence-id-391611

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  49. TFORF3104

    Plasmid
    #144580
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NKX2-5 (NKX2-5 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144580-plasmid-map-sequence-id-404280

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Showing: 8301 - 8350 of 159284 results