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167,150 results

Showing: 8451 - 8500 of 167150 results

  1. LEGO-AP1x3-GM55

    Plasmid
    #217414
    Purpose
    Luciferase expression vector with 3 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x3
    Depositor
    Peter Cockerill
    Insert
    Luciferase, GFP
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    3 AP-1 sites, Human -55 to +28 minimal CSF2 promo…
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217414-plasmid-map-sequence-id-434967

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  5. pCDF-mcherry1

    Plasmid
    #87144
    Purpose
    Constitutive E. coli mcherry Expression
    Depositor
    Michael Lynch
    Insert
    mcherry
    Expression
    Bacterial
    Promoter
    Constitutive proD
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87144-plasmid-map-sequence-id-176832

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  10. pSynSRE-Mut-T-Luc

    Plasmid
    #60490
    Purpose
    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988
    Depositor
    Timothy Osborne
    Article
    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.
    Insert
    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    Use
    Luciferase
    Tags
    Luciferase reporter
    Expression
    Mammalian
    Mutation
    Four point mutations (G->C or A->C) in the …
    Promoter
    Partial HMG-CoA synthase promoter (-324/-225) fus…
    Available Since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60490-plasmid-map-sequence-id-332535

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  13. pAAV.hSyn.iGluSnFR3.v857.SGZ

    Plasmid
    #178330
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn iGluSnFR3 v857.SGZ
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…
    Expression
    Mammalian
    Available Since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    178330-plasmid-map-sequence-id-353668

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  19. ePop

    Plasmid
    #50953
    Purpose
    Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations
    Depositor
    Lingchong You
    Article
    Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:
    Inserts
    E lysis protein
    LuxI
    LuxR
    Mutation
    Early stop codon introduced in mutated luxR resul…
    Available Since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50953-plasmid-map-sequence-id-454119

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  20. pHIS-GB1-BP1

    Plasmid
    #133427
    Purpose
    Human BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    SREBF1 (SREBF1 Human)
    Expression
    Bacterial
    Mutation
    SREBP1a amino acids 17-42 fused to GB1 solubilit…
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133427-plasmid-map-sequence-id-267726

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  29. pcDNA3-mRuby2

    Plasmid
    #40260
    Purpose
    RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Article
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has Service
    Cloning Grade DNA
    Insert
    mRuby2
    Tags
    His6 and Xpress
    Expression
    Mammalian
    Promoter
    CMV IE
    Available Since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    40260-plasmid-map-sequence-id-243381

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  30. TFORF3358

    Plasmid
    #144834
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    STAT3 (STAT3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144834-plasmid-map-sequence-id-404405

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  31. HCTR1-DuET

    Plasmid
    #213310
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HCTR1 (HCRTR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213310-plasmid-map-sequence-id-430848

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  34. pCR4-CTE

    Plasmid
    #36868
    Depositor
    Mario Chin
    Insert
    CTE
    Available Since
    Aug. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    36868-plasmid-map-sequence-id-46085

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  35. pcDNA5/FRT/TO-GAlpha15-RLuc8

    Plasmid
    #140984
    Purpose
    Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha15-RLuc8 (GNA15 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140984-plasmid-map-sequence-id-300775

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  41. p53-GFP

    Plasmid
    #11770
    Depositor
    Geoff Wahl
    Insert
    p53 (TP53 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    72R variant of p53
    Available Since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only
    11770-plasmid-map-sequence-id-163545

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  44. pLenti6.2_mCherry_mito Grx1-roGFP2

    Plasmid
    #155044
    Purpose
    Fluorescent redox reporter
    Depositor
    Vanessa LaPointe
    Insert
    Glutaredoxin-1 (GLRX Human)
    Use
    Lentiviral
    Tags
    mitochondrial targeting sequence and roGFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    155044-plasmid-map-sequence-id-311573

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  46. pUBC-SV40NLS-Pfv-Sapphire-PURO

    Plasmid
    #203654
    Purpose
    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    Lentiviral
    Tags
    Sapphire
    Available Since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203654-plasmid-map-sequence-id-402486

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Showing: 8451 - 8500 of 167150 results