175,587 results
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pAAV-TRE-DIO-FLPo
Plasmid#118027PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-DIO-FLPo
UseAAVPromoterTREAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_NEUROD1_P2A_Hygro_Barcode
Plasmid#120466PurposeBarcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Bxb1o_nlsFlag
Plasmid#119901PurposeExpresses Bxb1 integrase in mammalian cellsDepositorInsertMammalian codon optimized Bxb1 integrase
ExpressionMammalianPromoterCAGAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-CENP-A-mCherry
Plasmid#89767PurposeLentivirus destination vector for stable expression of mCherry-tagged kinetochore marker CENP-ADepositorAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLBH545_Tet-Cas14a1_Locus
Plasmid#112501PurposeExpresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14a1
UseCRISPRExpressionBacterialPromoterTetAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Raptor GFP
Plasmid#112744Purposeexpress Raptor-GFPDepositorAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mScarletI-Rab7
Plasmid#112955PurposeIn vivo visualization of Rab7 (can be used for colocalization studies)DepositorInsertRab7
TagsmScarlet-IExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-36: AAVS1-mEGFP (PGK)
Plasmid#114404PurposeHomology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFPDepositorInsertAAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
UseCRISPR; Donor templateExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV6-AC EJ7-GFP
Plasmid#113617PurposeReporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFPDepositorInsertEJ7-GFP variant of EGFP
ExpressionMammalianAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1_MeCP2(WT)
Plasmid#110186PurposeExpression of mouse MeCP2 (WT) in mammalian cellsDepositorInsertMeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)
TagsEGFPExpressionMammalianMutationwild typePromoterCMVAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZLCv2-3xFLAG-dCas9-HA-2xNLS
Plasmid#106357PurposeEmpty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitationDepositorTypeEmpty backboneUseCRISPR and LentiviralTags3XFLAG and HAExpressionMammalianPromoterU6Available SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA
Plasmid#105665Purposeexpresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialPromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30b-7+-SrtA
Plasmid#105602Purposeexpressed 7+ sortase A variant in E.coliDepositorInsertS.aureus Sortase A, 7+ mutant
TagsHis tagExpressionBacterialPromoterT7Available SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
DNMT3A-dCas9
Plasmid#100090PurposeDNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertDNMT3A (DNMT3A Human)
UseCRISPRTags3XFLag-NLS-DNMT3A-dCas9-NLSExpressionMammalianMutationincludes aa 602-912PromoterCMVAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAD-LyseR
Plasmid#99244PurposeFor autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorAvailable SinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CamKIIa_GFP
Plasmid#96941PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertGreen fluorescent protein
UseLentiviralPromoterCaMKIIaAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSAM_Cam
Plasmid#91570PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9-MQ1(Q147L)-EGFP
Plasmid#89793PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPR and LentiviralTags3*FLAG and T2A-EGFPExpressionMammalianMutationQ147L, CAA-->CTAPromoterhUbCAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1X-Nup93
Plasmid#87336PurposeTo express human Nup93 in mammalian cellsDepositorInsertNUP93 (NUP93 Human)
TagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_DLL1_WT
Plasmid#82108PurposeGateway Donor vector containing DLL1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-U6-gRNA-UbC-eGFP-P2A-Bsr
Plasmid#83925PurposeLentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastRDepositorInsertseGFP
Bsr
UseCRISPR and LentiviralTagsP2APromoterhUbCAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH Yap S127A
Plasmid#86497PurposeMammalian expression of FLAG-tagged Yap containing S127A mutationDepositorInsertYes associated protein 1 (YAP1 Human)
TagsFLAGExpressionMammalianMutationS127APromoterEF1aAvailable SinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB454
Plasmid#74836PurposeGateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N
Plasmid#80930PurposeExpresses Cas9N in mammalian cells.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFUSE2ss-CLIg-mK_M18
Plasmid#82358PurposeExpression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertTagsnoneExpressionMammalianPromoterhEF1-HTLV promAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
LeGO-lnc
Plasmid#80624PurposeLentiviral lncRNA OverexpressionDepositorInsertempty control
UseLentiviralPromoterSFFVAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
RHCglo
Plasmid#80169PurposeMinigene reporter for identification and analysis of cis elements and trans factors affecting pre-mRNA splicingDepositorInsertsegments of chicken cardiac troponin T (Tnnt2) introns 4 and 5 flanking an artifical exon
ExpressionMammalianAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001149198)
Plasmid#80248Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJJR82
Plasmid#75027PurposeEGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertEGFP^SEC^3xFlag
UseCRISPRTags3xFLAG and C. elegans codon optimized EGFPExpressionWormAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOS003-lenti-CMV-GCaMPer
Plasmid#65227PurposeER calcium sensor for lentivirus productionDepositorInsertGCaMPer
UseLentiviralTagscircularly permuted GFPExpressionMammalianMutationGCaMP3 (D324G, D360G, D397G, D435G)PromoterCMVAvailable SinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-hDDX5/17
Plasmid#71307PurposeInducible shRNA knockdown of both human DDX5 and DDX17 genesDepositorAvailable SinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-3xFLAG
Plasmid#69837PurposeThis plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorAvailable SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1 actin-3XNLS P2A mCherry
Plasmid#58475PurposeExpresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoterDepositorInsertsTagsmCherryExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE-60 Grx1-roGFP2-His
Plasmid#64799PurposeBacterial expression of His-tagged Grx1-roGFP2DepositorAvailable SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSiM24-eGFP
Plasmid#67883PurposePlant Gene expression Vector pSIM24 containing eGFP reporterDepositorInserteGFP
ExpressionPlantPromoterM24Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-NfiB
Plasmid#64900PurposeInducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.DepositorAvailable SinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
BPK2139
Plasmid#65776PurposeHuman expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAGDepositorInsertmammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
UseCRISPRTagsNLS-3xFLAGExpressionMammalianPromoterCAGAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mito-ABKAR
Plasmid#61509PurposeExpression of AMPK biosensor targeted to the mitochondriaDepositorInsertAMPK Biosensor
TagsMitochondrial targeting signal (DAKAP1-30)ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
M_ST1n_VPR
Plasmid#63799PurposeST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vectorDepositorInsertST1-dCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionMammalianPromoterCMVAvailable SinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZDonor-Nm-Cas9-gRNA-hU6
Plasmid#61366Purposeempty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNADepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U6Available SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgM/κ
Plasmid#61881PurposeExpresses HER2/neu receptor specific humanized IgM/κ antibody isotype (Trastuzumab-IgM/κ)DepositorInsertsHER2/neu receptor specific humanized Mu heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
ExpressionMammalianPromotermEF1 Prom and rEF1 PromAvailable SinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR4-Kras
Plasmid#61621PurposeNon-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activityDepositorInsertAKAR4-Kras
TagsC-terminal targeting sequence from Kras, Cerulean…ExpressionMammalianPromoterCMVAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
DR274-eGFP sgRNA
Plasmid#61051Purposefor generation of a eGFP specific sgRNA from a T7 promoterDepositorInsertEGFP sgRNA
UseCRISPRPromoterT7Available SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE-NLS-mCherry
Plasmid#60364PurposePlasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertNLS-mCherry
TagsNLS from SV40 T antigen and mCherryPromoterCMV-tetAvailable SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-ER-5a
Plasmid#56455PurposeLocalization: Endoplasmic Reticulum, Excitation: 488, Emission: 507DepositorAvailable SinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmCTIP2-N174
Plasmid#60858PurposeExpresses CTIP2 under the EF1a promoterDepositorAvailable SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP NLS Beta-Actin G13R
Plasmid#60615PurposeEncodes YFP and NLS tagged beta-actin with the G13R depolymerization mutationDepositorAvailable SinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-human OCT4 PE-SV40-Luc
Plasmid#52415PurposeLuciferase reporter plasmid for PE OCT4 enhancer activityDepositorAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
Opto-mFGFR1_204
Plasmid#58745PurposeExpresses Opto-mFGFR1 (light-activated receptor tyrosine kinase)DepositorInsertOpto-mFGFR1
ExpressionMammalianAvailable SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only
