162,536 results
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Plasmid#84683PurposeCan be used for the transformation of rickettsiaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only
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EGFP-PIK3CD
Plasmid#174190PurposeExpresses PIK3CD in mammalian cellsDepositorInsertPIK3CD (PIK3CD Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti SYN-dCas9-KRAB-MeCP2
Plasmid#155365PurposeExpresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoterDepositorInsertdCas9-KRAB-MeCP2
UseCRISPR and LentiviralTags3x FLAG and 7x HisExpressionMammalianMutationPromoterSYNAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CLASP2 512-650 9xS/A
Plasmid#24410DepositorInsertCLASP2 (512-650) 9xS/A (CLASP2 Human)
UseTagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant that c…PromoterAvailable sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF2242
Plasmid#141986PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGATA1 (GATA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA-PHLPP1 full length
Plasmid#37100PurposeExpresses HA tagged, full-length human PHLPP1 in mammalian cellsDepositorInsertPHLPP1 (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B
Plasmid#103002Purposenon-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV IGF1-FLAG
Plasmid#175170PurposeLentiviral expression of human Igf1-FLAGDepositorInsertIgf1 (IGF1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-PIPK1 gamma 90
Plasmid#22299DepositorInserthuman phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKJ0463
Plasmid#228715PurposeBacterial expression of epT7 RNAP; for protein production and purificationDepositorArticleInsertSumo_epT7
UseTagsExpressionBacterialMutationT3M/G47A/E643GPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
LPC-flag-REST-FS
Plasmid#41904DepositorInsertRE1-silencing transcription factor (REST Human)
UseRetroviralTagsFLAGExpressionMammalianMutationFS mutantPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)
Plasmid#169186PurposeTo express His-SUMO-nsp7 (SARS-CoV-2) in E. coliDepositorInsert14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
UseTags14His-SUMO (Ulp1-cleavable)ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETduet+p300HAT
Plasmid#157793PurposeExpression of p300 HAT domainDepositorInsertp300HAT (EP300 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_AMBRA1#2
Plasmid#174153PurposeLentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 geneDepositorInsertAMBRA (AMBRA1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-LCMV-Arm53b
Plasmid#15796DepositorInsertLCMVArm53b envelope glycoprotein
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDGE335
Plasmid#153244PurposeM3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Lifeact-EGFP
Plasmid#67303PurposeFluorescent indicator for F-actinDepositorInsertLifeact-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ACVR1
Plasmid#23822DepositorInsertACVR1 (ACVR1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
Lact-C2-GFP-p416
Plasmid#22853DepositorInsertLactadherin-C2
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
FUW-hSNCA-GFP
Plasmid#215489PurposeLentiviral expression and easy visualization of alpha-synuclein in mammalian cellsDepositorInsertalpha-synuclein (SNCA Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterUbcAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-mUbp43
Plasmid#12454DepositorInsertUBP43 (Usp18 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAIDFA-EF1a-NmScarlet-mAID
Plasmid#140615PurposeExpression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoterDepositorInsertOsTIR1 mutated F74A
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1P-iCAG.copGFP
Plasmid#66577PurposeAAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-AAVS1 TALENsDepositorInsertcopGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA Ef1alpha Puro-T2A-GFP
Plasmid#111596PurposesgRNA Ef1alpha Puro-T2A-GFPDepositorInsertmU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-IPP1-His
Plasmid#118978PurposeBacterial expression plasmid for Inorganic pyrophosphataseDepositorInsertinorganic diphosphatase IPP1 (IPP1 Budding Yeast)
UseTags6x-HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHR-dSV40-CTLA-4-mGFP
Plasmid#223594PurposeLentiviral expression of human CTLA4 with mGFP tag in mammalian cellsDepositorInsertCTLA4 (CTLA4 Human)
UseLentiviral; mammalian expressionTagsmGFPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TIGHT-CYCLINF-Tet-OFF
Plasmid#32977DepositorInsertCYCLIN F (CCNF Human)
UseLentiviralTagsFlag and mCherryExpressionMammalianMutationPromoterP-TIGHTAvailable sinceFeb. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-GFP-NMHC II-A
Plasmid#11347DepositorInsertNMHC II-A (MYH9 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCI NL4-3 Nef-HA-WT
Plasmid#24162DepositorInsertNef (nef HIV)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pS448∙CsR
Plasmid#122096PurposeCRISPR/Cas9 counterselection in Gram-negative bacteriaDepositorInsertsCas9
sgRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPem7 and PmAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
C3AR1-DuET
Plasmid#213191PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertC3AR1 (C3AR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-FXR1-isoform b
Plasmid#225449PurposeExpress EGFP-fusion protein of FXR1 isoform bDepositorInsertFXR1 isoform b (FXR1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-Met15
Plasmid#69198Purposecomplementation of yeast auxotrophic marker MET15DepositorInsertMET15
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_zeo_sgTP53
Plasmid#183180PurposepLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53DepositorInsertsgTP53 (TP53 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50454PurposeDouble floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoterDepositorUseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pXG260
Plasmid#141282PurposeCMV:EGFP (as control for pXG237)DepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE337
Plasmid#153246PurposeM4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
piRFP
Plasmid#31857Purposea phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imagingDepositorHas ServiceCloning Grade DNAInsertiRFP
UseTagsExpressionMammalianMutationT2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…PromoterCMVAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TET1-dCas9
Plasmid#235593PurposeTet1CD fused to N-terminus of dCas9DepositorInsertTET1-dCas9 (TET1 Human, SpCas9 is from Streptococcus pyogenes)
UseCRISPRTags3xNLS, TET1 CD, and tagBFPExpressionMammalianMutationPromoterEF1aAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE19
Plasmid#90361PurposeWnt - TCF/LEF gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterWntAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
alpha7nACHR_Nacho_GCAMP7s
Plasmid#182940Purposealpha7 nACHR expression in mammalian cell lines with calcium reporterDepositorInsertNACH7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mito-CanlonicSF/pCMV-myc-mito
Plasmid#224465PurposeTo explore mitochondrial lactate dynamicsDepositorInsertCanlonicSF
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC18-mini-Tn7T
Plasmid#64954Purposemini-Tn7 base vector with transcriptional terminatorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRK5 c-Fyn
Plasmid#16032DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_5SA-PolyA
Plasmid#112285PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)DepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with 5 Serines (61, 109, 127, 1…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-Gal4-Vp16-Crys-CFP
Plasmid#97144PurposeFor Gal4/UAS expression systemDepositorInsertGal4-Vp16
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-GFP-Fishell-1
Plasmid#83900PurposeGFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer elementDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserteGFP
UseAAVTagsExpressionMutationPromotermDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-NanoLuc
Plasmid#189975PurposePart 3-4-5 plasmid to introduce 3xFLAG-NanoLuc into circRNAsDepositorInsertNanoLuc
UseCloningTags3xFLAGExpressionMutationPromoterN/AAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only