19,210 results
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pQLinkH
Plasmid#13667DepositorInsertmulti cloning site
UseCo-expressionTagsHis, TEV, and attB1ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pAV5S-F30-2xdBroccoli
Plasmid#66845PurposeExpresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cellsDepositorInsertTwo dimeric Broccoli aptamers in the F30 scaffold
UseTags5S rRNAExpressionMammalianMutationPromoter5SAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY
Plasmid#64915PurposeEncodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertsPolyspecific aminoacyl-tRNA Synthetase
Amber Suppression tRNA
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6/H1Available sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFD116
Plasmid#124769PurposepFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorInsertsPtet
dcas9
PpflB sgRNA BsaI
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-Y705F-STAT3
Plasmid#71445PurposeRetroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationY705FPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET17_K560_GFP_His
Plasmid#15219DepositorInsertHuman neuronal kinesin heavy chain (KIF5B Human)
UseTagsGFP and HisExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAW212
Plasmid#172196PurposeP1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution with different restriction sitesDepositorInsertp10B2-AT110
UseTagsExpressionYeastMutationPromoter10B2 (P1 specific promoter)Available sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-KIRIN1
Plasmid#107414PurposeBacterial Expression plasmid for potassium ion raitometric indicator KIRIN1DepositorInsertKIRIN1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-p18-HA
Plasmid#42338DepositorInsertp18 (LAMTOR1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKIKOlacZCm
Plasmid#46764PurposeE.coli genomic integration at lacZDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHD-DsRed-attP-w+
Plasmid#80898PurposeVector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.DepositorInsertsattP
LoxP-3xP3-DsRed-LoxP
GMR-white
UseHigh copy, amp resistanceTagsExpressionMutationPromoter3xP3 and GMR::hsp70Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
hWIP1 FLAG
Plasmid#28105DepositorInsertWIP1 (PPM1D Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-hTRAF3
Plasmid#44032DepositorInsertTNF receptor-associated factor 3 (TRAF3 Human)
UseTags1xHAExpressionMammalianMutationS393GPromoterAvailable sinceApril 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
426Gal-FUS-YFP
Plasmid#29592DepositorInsertFUS (FUS Human)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGreenPuro-shRNA-Stx3S-C4
Plasmid#99742Purpose3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.DepositorInsertshRNA against Stx3S
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3s-Sniper-Cas9
Plasmid#113912PurposeExpresses Sniper-Cas9 with CMV promoterDepositorInsertSniper-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pGL4.0-TERT G250A
Plasmid#84925Purposeluciferase reporter for mutant TERT promoterDepositorInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationG250APromoterTERTAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYLsgRNA-OsU6c
Plasmid#66197Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterOsU6cAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-mSncg-EGFP
Plasmid#153163PurposeMouse gamma-synuclein (mSncg) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromotermSncgAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pGEM-HE-h_bPAC_cmyc
Plasmid#28134Purposeoptogenetic tool for light modulation of cellular cAMPDepositorInserthumanized photoactivated adenylyl cyclase
UseIn vitro transcriptionTagsMycExpressionMutationPromoterAvailable sinceJune 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-EGFP-F
Plasmid#73014PurposeRetroviral expression of EGFP-FDepositorInsertEGFP-F
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-hChR2(H134R)-EYFP
Plasmid#27054PurposeAAV expression of humanized ChR2(H134R) fused to EYFP, for optogenetic excitationDepositorInserthChR2(H134R)
UseAAVTagsEYFPExpressionMammalianMutationH134RPromoterGFAPAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pBabe-puro largeTcDNA
Plasmid#14088DepositorInsertLarge T antigen (SV40gp6 simian virus)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-GFP-PALB2
Plasmid#71113Purposemammalian expession of GFP-PALB2DepositorInsertPALB2 (PALB2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMV/TOAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBJ5 hBRG1
Plasmid#17873DepositorInsertBrg (SMARCA4 Human)
UseTagsExpressionMammalianMutationR1608QPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAGmCherry-gRNA
Plasmid#87110PurposegRNA cloning vector with CAGmCherryDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DEST53 PINK1 N-GFP
Plasmid#13315DepositorInsertPINK1 (PINK1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-E2F4
Plasmid#10914DepositorInsertE2F4 (E2F4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HaloTag7-SnoopLigase
Plasmid#105627PurposePlasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7DepositorInsertHaloTag7-SnoopLigase
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEDA5_GFPmut3_Y66H
Plasmid#80085PurposePositive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.DepositorInsertGFPmut3_Y66H
UseTags6x-His tagExpressionBacterialMutationchanged Tyrosine 66 to HistidinePromoterproBAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 CMV sTurboID (C)-Arrestin-GFP
Plasmid#153010PurposeExpresses split-TurboID C-terminal fragment fused to arrestinDepositorInsertsTurboID (C)(G74)-Arrestin
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
FU-tet-o-hKLF4
Plasmid#19777DepositorInsertKlf4 (KLF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Vinculin-N-21
Plasmid#55160PurposeLocalization: Focal Adhesions, Excitation: 587, Emission: 610DepositorInsertVinculin (VCL Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSEM238
Plasmid#161515PurposeNeuronal expression of histamine-sensitive chloride channel for negative selectionDepositorInsertpEXP[Psnt-1 | histamine | rpl-3 UTR]
UseTagsExpressionWormMutationPromoterPsnt-1Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV-IFP2.0 T2A HO1
Plasmid#59427PurposeExpress infrared fluorescent protein IFP2.0 plus HO1 enzymeDepositorInsertIFP2.0T2AHO1 IRES EGFP
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp3 C163A-myc
Plasmid#11814DepositorInsertCaspase 3 catalytic mutant (CASP3 Human)
UseTagsMycExpressionMammalianMutationC163APromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
mApple-VE-Cadherin-N-10
Plasmid#54959PurposeLocalization: Tight Junctions, Excitation: 568, Emission: 592DepositorInsertVE-Cadherin (CDH5 Human)
UseTagsmAppleExpressionMammalianMutationI503TPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNO286-3
Plasmid#107746PurposeKey genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)DepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationDeleted AA 223 and 512PromoterJ23106 and J23108Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMP71-hCD155
Plasmid#118630Purposehuman CD155 coding sequence subcloned into pMP71 retroviral plasmidDepositorInsertCD155 (PVR Human)
UseTagsExpressionMammalianMutationPromoterLTRAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL11017 (pICH47732::NOSp-BAR-NOST)
Plasmid#51145PurposeConfers Basta resistance in plantaDepositorInsertNOSp-BAR-NOST
UseTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmCherry1
Plasmid#89986PurposeLong stokes-shift variant of red fluorescent protein mCherryDepositorInsertLSSmCherry1
UseTags6xHis-T7-XpressExpressionBacterialMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX311 MCL1
Plasmid#117729PurposeOpen reading frame vector encoding MCL1DepositorInsertMCL1L (MCL1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP7c variant 1513
Plasmid#105320PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7c variant 1513
UseTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+mScarlet-C Cloning Vector
Plasmid#128147PurposeTo express a protein of interest fused to the C-terminus of mScarletDepositorTypeEmpty backboneUseTagsmScarlet fusionExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
MCP-M-MLV-RT
Plasmid#181799PurposeMCP-M-MLV-RT (MS2-N55K-MMLVRT)DepositorInsertMCP-M-MLV-RT
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only
