178,651 results
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Plasmid#96918Purposefor CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121DepositorInsertdCas9
UseLentiviralTagsKRABExpressionMammalianMutationdCas9PromoterEF1aAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT3TS-nCas9n
Plasmid#46757Purposeexpression of an optimized Cas9 for genome editing in zebrafishDepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionBacterialPromoterT3Available sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
7TGC
Plasmid#24304DepositorPublicationInsert7xTcf-eGFP // SV40-mCherry
UseLentiviralMutationEGFP is GFP with S65T and F64L mutationsAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSIRV-NFAT-eGFP
Plasmid#118031PurposeFluorescent reporter for NFAT activationDepositorInserteGFP
UseRetroviralPromoterminimal promoter with NFAT binding siteAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCHAC-mt-mKeima
Plasmid#72342Purposeretrovirus construct for stable expressing mt-mKeimaDepositorInsertmt-mKeima
UseRetroviralAvailable sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRGEB32
Plasmid#63142PurposeExpress sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.DepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterRice snoRNA U3 promoter for gRNA/PTG expression a…Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD5
Plasmid#73914Purposemultiplex gRNA plasmidDepositorInsertdU6-3:tRNA:gRNA:tRNA:gRNA
UseCRISPRExpressionInsectPromoterdU6:3Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-CMV-NES-jRCaMP1a
Plasmid#61562PurposeRed fluorescent calcium sensor proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNES-jRCaMP1a
Tagsnuclear export signalExpressionMammalianPromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC1-HyPer-Red
Plasmid#48249PurposeGenetically encoded hydrogen peroxide indicator with red fluorescence.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHYPER-RED
ExpressionMammalianPromoterCMVAvailable sinceOct. 31, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VinculinTS
Plasmid#26019DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVinculin tension sensor
ExpressionMammalianAvailable sinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR
Plasmid#60229PurposeExpresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Cre recombinase
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HAExpressionMammalianPromoterCBh and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-myc-CUL3
Plasmid#19893DepositorPublicationAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TagRFP-T-EEA1
Plasmid#42635DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEEA1 (EEA1 Human)
TagsTagRFP-TExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLifeAct-mTurquoise2
Plasmid#36201PurposeIn vivo visualization of actin cytoskeleton (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeAct
ExpressionMammalianPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUW-tetO-hMYC
Plasmid#20723DepositorAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI flag PCAF
Plasmid#8941DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCAF (KAT2B Human)
TagsflagExpressionMammalianAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPRv2 puro
Plasmid#98290PurposeVariant of lentiCRISPRv2 that confers puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-puro-cMyc
Plasmid#46970Purposelentiviral expression of c-MycDepositorAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET His6 Sumo TEV LIC cloning vector (1S)
Plasmid#29659DepositorTypeEmpty backboneTags6xHis, SUMO, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EF.hICN1.CMV.GFP
Plasmid#17623PurposeDepositorPublicationAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3.1(+)-CMV-bArrestin2-TEV
Plasmid#107245PurposeVector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertARRB2
TagsTEV(NIA), tobacco etch virus NIAExpressionMammalianPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti sgRNA(MS2)_puro optimized backbone
Plasmid#73797Purposeoptimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKDsgRNA-p15
Plasmid#62656PurposeArabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmidDepositorInsertexo, beta, gam, sgRNA
ExpressionBacterialAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-tdTomato (codon diversified)
Plasmid#59462PurposeAAV-mediated expression of tdTomato (codon diversified) under the CAG promoterDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.S, AAV PHP.eB, and AAV RetrogradeInserttdTomato
UseAAVExpressionMammalianMutationcodon diversifiedPromoterCAGAvailable sinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3-U6-sgRNA-PGK-puromycin
Plasmid#51133Purposeexpresses sgRNA from the U6 promoter.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-FLAG-LKB1
Plasmid#8590DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLKB1 (STK11 Human)
TagsFLAGExpressionMammalianAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-EEA1 wt
Plasmid#42307DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEEA1 (EEA1 Human)
TagsGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJFRC19-13XLexAop2-IVS-myr::GFP
Plasmid#26224DepositorInsertmyr::GFP
ExpressionInsectAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVET-tTR-KRAB
Plasmid#11644PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthEF-1alpha, GFP, tTR-KRAB, Tet-on
UseLentiviralExpressionMammalianAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSicoR
Plasmid#11579PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiExpressionMammalianAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by