8,789 results

1. pMOS023: Peredox NADH/NAD+ sensor (cytosolic)

   Plasmid

   #163060

   Purpose

   Peredox NADH/NAD+ sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   Peredox
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p414-TEF1p-Cas9-CYC1t

   Plasmid

   #43802

   Purpose

   A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   Human Optimized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available Since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti CMV Puro DEST (w118-1)

   Plasmid

   #17452

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCherry.90 alpha

   Plasmid

   #108222

   Purpose

   Mammalian expression vector for mCherry fusion to human Hsp90 alpha

   Depositor

   Didier Picard

   Article

   Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

   Insert

   mCherry-Hsp90a (HSP90AA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLJM5-WT PHGDH

   Plasmid

   #83901

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Flag-HA-USP18

   Plasmid

   #22572

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP18 (USP18 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC57-mitoLbNOX

   Plasmid

   #74448

   Purpose

   This plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

   Insert

   mitoLbNOX
   

   Use

   Tags

   Flag tag with linker and mitochondrial targeting …

   Expression

   Mutation

   Human codon optimized

   Promoter

   Available Since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-GFP

   Plasmid

   #160697

   Purpose

   Expresses GFP in mammalian cells

   Depositor

   Christine Mayr

   Article

   Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. dCas13d-dsRBD

   Plasmid

   #154938

   Purpose

   BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

   Depositor

   Alice Ting

   Article

   Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

   Insert

   BPNLS-dRfxCas13d-dsRBD-BPNLS-HA
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MXS_BidirectionalCAG

    Plasmid

    #78175

    Purpose

    Bidirectional CAG promoter (compatible with the MXS Chaining Kit)

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    Bidirectional CAG Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDEST17-Cdc34a

    Plasmid

    #18674

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Cdc34A (CDC34 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3 Flag beta-2-adrenergic-receptor

    Plasmid

    #14697

    Depositor

    Robert Lefkowitz

    Article

    Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    B2AR (ADRB2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Mfn2-Myc

    Plasmid

    #23213

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Mfn2 (Mfn2 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. circRNA-synIRES-R25-NanoLuc

    Plasmid

    #188116

    Purpose

    Template plasmid to transcribe circular RNA encoding NanoLuc

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Tet-O-FUW-EGFP

    Plasmid

    #30130

    Depositor

    Marius Wernig

    Article

    Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

    Insert

    Enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. IFN-Beta_pGL3

    Plasmid

    #102597

    Purpose

    IFN-beta promoter driving luciferase. Use in IFN reporter assay.

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    Human IFN-Beta promoter (IFNB1 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNIC28-Bsa4

    Plasmid

    #26103

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CMV-mCherry-(F30-2xPepper)10

    Plasmid

    #129401

    Purpose

    mCherry mRNA reporter tagged with (F30-2xPepper)10

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    mCherry-(F30-2xPepper)10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MEK1-GFP

    Plasmid

    #14746

    Depositor

    Rony Seger

    Article

    Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. anti-FLAG M2 heavy chain

    Plasmid

    #175359

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 heavy chain
    

    Use

    Tags

    His8 tag and N-terminal secretion signal peptide …

    Expression

    Mammalian

    Mutation

    L51I (see paper)

    Promoter

    CMV

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. htrpC6-YFP

    Plasmid

    #21084

    Depositor

    Craig Montell

    Article

    Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

    Insert

    TrpC6 (TRPC6 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUAS-NanoLuc

    Plasmid

    #87696

    Purpose

    Gal4VP16 driven expression of NanoLuc

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NanoLuc
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEF5-FRT-TagRFP-T-IFT88

    Plasmid

    #61684

    Purpose

    Mammalian expression of IFT88 fused with N terminal TagRFP.T tag

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    IFT88 (Ift88 Mouse)
    

    Use

    Tags

    TagRFP.T

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLEX_305

    Plasmid

    #41390

    Purpose

    Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag

    Depositor

    David Root

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pORI28

    Plasmid

    #71595

    Purpose

    EC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.

    Depositor

    Jan Kok, Todd Klaenhammer

    Article

    Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

    Type

    Empty backbone

    Use

    Integration vector for bacteria

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-GFP

    Plasmid

    #11150

    Purpose

    Mammalian expression vector for expression of GFP (CAG promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    EGFP from Aequorea victoria
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. anti-FLAG M2 light chain

    Plasmid

    #175358

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 light chain

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 light chain
    

    Use

    Tags

    N-terminal secretion signal peptide (MEAPAQLLFLLL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HA-HIF1alpha-wt-pBabe-puro

    Plasmid

    #19365

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt human cDNA

    Promoter

    Available Since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. dCas9-KRAB-MeCP2

    Plasmid

    #110821

    Purpose

    Improved dCas9 repressor-dCas9-KRAB-MeCP2

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mCherry-Calreticulin-N-16

    Plasmid

    #55006

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Calreticulin (CALR Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid

    #118154

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    EF1a core and U6

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLKO.1 hygro

    Plasmid

    #24150

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.

    Depositor

    Bob Weinberg

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA

    Available Since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-YFP

    Plasmid

    #13033

    Purpose

    Mammalian expression vector for cloning your gene fused to YFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Yellow fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a-sfGFP

    Plasmid

    #85492

    Purpose

    pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA-HA-ER WT

    Plasmid

    #49498

    Purpose

    Expresses HA-tagged ER wild-type in mammalian cells

    Depositor

    Sarat Chandarlapaty

    Article

    Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLX301

    Plasmid

    #25895

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAG-Brainbow3.0

    Plasmid

    #45176

    Depositor

    Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Insert

    pCAG-Brainbow3.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRK5-HA-Ubiquitin-WT

    Plasmid

    #17608

    Purpose

    Mammalian expression of HA tagged ubiquitin

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAV pCAG-FLEX-EGFP-WPRE

    Plasmid

    #51502

    Purpose

    Can be used to generate AAV virus that will express EGFP in the presence of Cre

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSIH1-puro-STAT3 shRNA

    Plasmid

    #26596

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Insert

    STAT3 shRNA (STAT3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge